Ngwa Nchọpụta maka Alpha-fetoprotein(nnwale immunochromatographic fluorescence)
Maka ojiji nchọpụta n'ime vitro naanị
Biko gụọ ihe e tinyere na ngwugwu a nke ọma tupu e jiri ya, ma gbasoo ntuziaka ndị a nke ọma. A gaghị ekwe nkwa na nsonaazụ nnwale ga-adabere na ntụkwasị obi ma ọ bụrụ na e nwere ihe ọ bụla na-adịghị mma na ntuziaka dị na ngwugwu a.

Ebumnobi Ojiji

Ngwa Nchọpụta maka Alpha-fetoprotein (nnwale fluorescence immunochromatographic) bụ nnwale fluorescence immunochromatographic maka nchọpụta ọnụọgụ nke Alpha-fetoprotein (AFP) na serum ma ọ bụ plasma mmadụ, nke a na-ejikarị eme nchọpụta enyemaka, mmetụta ọgwụgwọ na amụma nke ọrịa kansa hepatocellular mbụ. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma. Emebere ule a maka ojiji ndị ọkachamara ahụike naanị.

Nchịkọta

Alpha-fetoprotein (AFP) bụ otu n'ime ihe nrịbama etuto a na-ejikarị eme ihe. Ọ bụ glycoprotein nwere oke molekul nke 70,000 na shuga nke 4%. Ọ bụ imeju nwa ebu n'afọ na-emepụta ya, wee soro ya na akpa nkochi ime nwa. Nwa ebu n'afọ malitere imepụta ihe ruo izu isii, na-eru oke kachasị elu nke izu iri na abụọ ruo iri na ise, njupụta serum nke 1 ruo 3 g/L, na ọbara eriri afọ mgbe a mụrụ ya nke 10 ruo 100 mg/L; afọ 1 ruo 2 mgbe a mụsịrị ya ruo ọkwa okenye; Afọ ime nkịtị nwere ike iru 90 ruo 500 ng/mL n'etiti; Ọnụọgụ AFP nkịtị n'ime serum mmadụ dị n'etiti 2 na 8 ng/mL, mana ọtụtụ ọrịa, ọkachasị hepatitis, na-emetụta uru AFP.

ỤKPỤRỤ NKE ỤZỌ A

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi AFP na mpaghara nnwale ahụ na mgbochi ewu IgG antibody na mpaghara njikwa. A na-ekpuchi paadị label ahụ site na iji fluorescence nke ejiri akara anti AFP na oke bekee IgG kpuchie tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, antigen AFP dị na ihe nlele ahụ na-ejikọta ya na mgbochi AFP antibody nke ejiri akara fluorescence mee, ma mepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri, mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na mgbochi AFP mkpuchi antibody, na-emepụta mgbagwoju anya ọhụrụ. Ọkwa AFP na-ejikọta nke ọma na mgbaàmà fluorescence, a pụkwara ịchọpụta ntinye nke AFP na ihe nlele ahụ site na nnwale immunoassay fluorescence.

Ihe ndị na-eme ka ihe dị nro na ihe ndị e ji mee ihe dị iche iche

Ihe mejupụtara ngwugwu 25T:

. Kaadị ule n'otu n'otu nke ejiri ihe nhicha mmiri 25T kpuchie akpa ahụ
.Ihe ndị na-agbaze ihe nlele 25T
.Ngwakọta ihe ntinye 1

A CHỌRỌ IHE E JI MEE YA MA E NWEGHỊ YA
Ihe nlele nchịkọta akpa, ihe ngụ oge
Nchịkọta na Nchekwa ihe nlele
1. Ihe nlele ndị a nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2. Dịka usoro ọkọlọtọ si dị, a pụrụ idobe ihe nlele ọbara ma ọ bụ plasma n'ime friji na okpomọkụ 2-8℃ ruo ụbọchị asaa, ebe a na-echekwa ihe nlele ahụ n'okpuru -15°C ruo ọnwa isii.
3. Ihe nlele niile zere usoro oyi kpọnwụrụ akpọnwụ.

Usoro Nnwale
Biko gụọ akwụkwọ ntuziaka ọrụ ngwa ahụ na ihe e tinyere na ngwugwu tupu ị nwaa ya.
1. Debe ihe niile e ji eme ihe na ihe nlele n'akụkụ okpomọkụ ụlọ.
2. Mepee Portable Immune Analyzer (WIZ-A101), tinye paswọọdụ akaụntụ dịka usoro ọrụ nke ngwa ahụ si dị, wee tinye interface nchọpụta.
3. Lelee koodu dentification iji kwado ihe nnwale ahụ.
4. Wepụ kaadị ule ahụ n'ime akpa foil ahụ.
5. Tinye kaadị ule ahụ n'ime oghere kaadị ahụ, lelee koodu QR ahụ, wee chọpụta ihe nnwale ahụ.
6. Tinye ihe nlele serum ma ọ bụ plasma 20μL na ihe nlele mmiri, wee gwakọta ya nke ọma.
7. Tinye ihe ngwọta ihe nlele 80μL iji nwalee nke ọma nke kaadị ahụ.
8. Pịa bọtịnụ "ule ọkọlọtọ", mgbe nkeji iri na ise gachara, ngwaọrụ ahụ ga-achọpụta kaadị nnwale ahụ na akpaghị aka, ọ nwere ike ịgụ nsonaazụ sitere na ihuenyo ngosipụta nke ngwaọrụ ahụ, ma dekọọ/bipụta nsonaazụ nnwale ahụ.
9. Lee ntuziaka nke Portable Immune Analyzer (WIZ-A101).

Uru A Na-atụ Anya Ya

AFP＜＜10ng/mL
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya nke na-anọchite anya ọnụọgụ ndị ọrịa ya.

Nsonaazụ na Nkọwa nke Ule
.Ihe ndị a dị n'elu bụ nsonaazụ nke ule AFP reagent, a na-atụkwa aro ka ụlọ nyocha ọ bụla guzobe ọtụtụ uru nchọpụta AFP dabara adaba maka ndị bi na mpaghara a. Nsonaazụ ndị a dị n'elu bụ maka ntụaka naanị.
. Nsonaazụ nke usoro a metụtara naanị oke ntụaka e guzobere n'ụzọ a, ọ dịghịkwa ntụnyere kpọmkwem na ụzọ ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ịkpata mmejọ na nsonaazụ nchọpụta, gụnyere ihe ndị metụtara teknụzụ, mmejọ arụmọrụ na ihe atụ ndị ọzọ.

Nchekwa na nkwụsi ike
1. Ngwa a ga-adịru ọnwa iri na asatọ site na ụbọchị e mepụtara ya. Debe ngwa ndị a na-ejighị na okpomọkụ 2-30°C. EKPERELA FRỊA. Ejila ya karịa ụbọchị njedebe.
2. Emeghela akpa ahụ e mechiri emechi ruo mgbe ị dị njikere ime nnwale, a na-atụkwa aro ka e jiri ule ahụ otu ugboro mee ihe n'okpuru ebe achọrọ (okpomọkụ 2-35℃, iru mmiri 40-90%) n'ime nkeji iri isii ozugbo enwere ike.
3. A na-eji ihe nlele sample ozugbo emepechara ya.

ỊDỌ AKA NA NTỊ NA ỊKPACHICHE
.A ga-emechi ngwa ahụ ma chebe ya pụọ ​​na mmiri mmiri.
. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma.
A ga-ewere ihe nlele niile dị ka ihe nwere ike imetọ ihe.
.EJILA ihe na-eme ka ihe ghara ịdị irè.
. EJIGHỊ agbanwe ihe ndị na-emegharị ihe n'etiti ngwa ndị ahụ na ọtụtụ ihe dị iche iche.
.EJILA kaadị ule na ihe ndị ọzọ a na-atụfu atụfu eme ihe ọzọ.
.Ịrụ ọrụ na-ezighi ezi, oke ma ọ bụ obere ihe nlele nwere ike ibute mgbanwe nsonaazụ.

LNṄỤRỤ
Dịka ọ dị na nnwale ọ bụla ejiri ọgwụ mgbochi òké mee, o nwere ike inwe nsogbu site na ọgwụ mgbochi òké mmadụ (HAMA) na ihe nlele ahụ. Ihe nlele sitere n'aka ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchọpụta ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ihe nlele ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ nsonaazụ na-adịghị mma.
.Nsonaazụ ule a bụ naanị maka ntụaka ahụike, ekwesịghị ịbụ naanị ntọala maka nchọpụta na ọgwụgwọ ahụike, njikwa ahụike nke ndị ọrịa kwesịrị ịtụle nke ọma yana mgbaàmà ya, akụkọ ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ.
A na-eji ihe a eme ihe naanị maka ule serum na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dịka ọnụ mmiri na mmamịrị na ihe ndị ọzọ.

ÀGWÀ ÀGWÀ Arụmọrụ

RNtụaka
1. Hansen JH, na ndị ọzọ. Mgbochi HAMA na Mbelata Mgbochi Mgbukpọ nke Dabere na Mgbukpọ Monoclonal [J].J nke Clin Immunoassay, 1993,16:294-299.
2. Levinson SS. Ọdịdị nke Ọgwụ Mgbochi Heterophilic na Ọrụ dị na Mgbochi Immunoassay[J].J nke Clin Immunoassay, 1992,15:108-114.

Isi ihe nnọchianya ejiri mee ihe:

	Ngwaọrụ Ahụike Nchọpụta In Vitro
	Onye nrụpụta
	Chekwaa na okpomọkụ 2-30℃
	Ụbọchị mmebi
	Ejila ya ọzọ
	ỊDỌ AKA NA NTỊ
	Lelee Ntuziaka Maka Ojiji

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