Matenda Kit cha Alpha-fetoprotein(kuyesa kwa fluorescence immunochromatographic)
Kugwiritsa ntchito poyezetsa matenda m'thupi kokha
Chonde werengani bwino phukusili musanagwiritse ntchito ndipo tsatirani malangizo mosamala. Kudalirika kwa zotsatira za mayeso sikungatsimikizidwe ngati pali kusiyana kulikonse kuchokera ku malangizo omwe ali mu phukusili.

KUGWIRITSA NTCHITO KOFUNIKA

Chida Chodziwira Matenda cha Alpha-fetoprotein (kuyesa kwa immunochromatographic kwa kuwala) ndi njira yoyesera ya fluorescence immunochromatographic yodziwira kuchuluka kwa Alpha-fetoprotein (AFP) mu seramu yamagazi ya anthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka pozindikira matenda, kuchiritsa komanso kutsimikizira kwa khansa ya chiwindi. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Mayesowa ndi a akatswiri azaumoyo okha.

CHIDULE

Alpha-fetoprotein (AFP) ndi imodzi mwa zizindikiro zodziwika bwino za chotupa. Ndi glycoprotein yokhala ndi kulemera kwa mamolekyulu 70,000 ndi shuga wa 4%. Imapangidwa makamaka ndi chiwindi cha fetal, kutsatiridwa ndi yolk sac. Mwana wosabadwayo anayamba kupanga kwa milungu 6, kufika pachimake pa masabata 12 mpaka 15, kuchuluka kwa seramu ya 1 mpaka 3 g/L, ndi magazi a umbilical cord pakubadwa kwa 10 mpaka 100 mg/L; chaka chimodzi mpaka ziwiri kuchokera pamene kubadwa kwa mwana mpaka kufika pa msinkhu wa munthu wamkulu; Mimba yachibadwa imatha kufika 90 mpaka 500 ng/mL pakati; Kuchuluka kwa AFP m'magazi a munthu kumakhala pakati pa 2 ndi 8 ng/mL, koma matenda ambiri, makamaka chiwindi, amakhudza kuchuluka kwa AFP.

MFUNDO YA NJIRAYI

Chipolopolo cha chipangizo choyesera chimakutidwa ndi anti-AFP antibody pamalo oyesera ndipo anti-IgG antibody ya mbuzi pamalo olamulira. Mapepala olembera amakutidwa ndi anti-AFP antibody yolembedwa kuti fluorescence ndi anti-IgG ya kalulu pasadakhale. Poyesa chitsanzo chabwino, anti-AFP mu chitsanzocho imaphatikizana ndi anti-AFP antibody yolembedwa kuti fluorescence, ndikupanga chisakanizo cha chitetezo chamthupi. Pogwiritsa ntchito immunochromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zidadutsa pamalo oyesera, zimaphatikizana ndi anti-AFP coating antibody, zimapanga zovuta zatsopano. Mlingo wa AFP umagwirizana bwino ndi chizindikiro cha fluorescence, ndipo kuchuluka kwa AFP mu chitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.

ZOPANGIRA NDI ZIPANGIZO ZOPEREKEDWA

Zigawo za phukusi la 25T：

Khadi loyesera lokhala ndi foil payokha lokhala ndi desiccant 25T
Zitsanzo za zosungunulira 25T
. Phukusi lolowera 1

Zipangizo zofunika koma siziperekedwa
Chidebe chosonkhanitsira zitsanzo, nthawi
Zitsanzo Zosonkhanitsira ndi Kusunga
1. Zitsanzo zomwe zayesedwa zitha kukhala seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2. Kutengera ndi njira zodziwika bwino, sonkhanitsani chitsanzo. Seramu kapena plasma chitsanzo chikhoza kusungidwa mufiriji pa 2-8℃ kwa masiku 7 ndikusungidwa pansi pa -15°C kwa miyezi 6.
3. Zitsanzo zonse kupewa kuzizira ndi kusungunula madzi.

NJIRA YOYESERA
Chonde werengani buku la malangizo ogwiritsira ntchito chipangizocho ndi phukusi la phukusi musanayese.
1. Ikani pambali zinthu zonse zoyeretsera ndi zitsanzo kutentha kwa chipinda.
2. Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi olowera mu akaunti malinga ndi njira yogwirira ntchito ya chidacho, ndikulowetsa mawonekedwe ozindikira.
3.Scan code yodziwitsa kuti mutsimikizire chinthu choyesera.
4. Tulutsani khadi loyesera m'thumba la foil.
5. Ikani khadi loyesera mu malo oikamo khadi, sikani QR code, ndikuzindikira chinthu choyesera.
6. Onjezani 20μL seramu kapena chitsanzo cha plasma ku chitsanzo cha diluent, ndipo sakanizani bwino.
7. Onjezani yankho la chitsanzo cha 80μL ku chitsime cha chitsanzo cha khadi.
8. Dinani batani la "standard test", patatha mphindi 15, chidacho chidzazindikira khadi loyesera lokha, chimatha kuwerenga zotsatira kuchokera pazenera lowonetsera chidacho, ndikulemba/kusindikiza zotsatira za mayeso.
9. Onani malangizo a Portable Immune Analyzer (WIZ-A101).

MFUNDO ZOYEMBEKEZEDWA

AFP: ＜10ng/mL
Ndikofunikira kuti labotale iliyonse ikhazikitse kuchuluka kwake komwe kumayimira odwala ake.

Zotsatira za Mayeso ndi Kutanthauzira
Deta yomwe ili pamwambapa ndi zotsatira za mayeso a AFP reagent, ndipo akulangizidwa kuti labotale iliyonse ikhazikitse mitundu yosiyanasiyana ya AFP yowunikira yoyenera anthu m'derali. Zotsatira zomwe zili pamwambapa ndi zongogwiritsidwa ntchito pongogwiritsa ntchito.
Zotsatira za njira iyi zimagwira ntchito kokha pa ma reference ranges omwe akhazikitsidwa mu njira iyi, ndipo palibe kufananizidwa mwachindunji ndi njira zina.
Zinthu zina zingayambitsenso zolakwika pakupeza zotsatira, kuphatikizapo zifukwa zaukadaulo, zolakwika pakugwira ntchito ndi zinthu zina zachitsanzo.

KUSUNGA NDI KUKHALA BWINO
1. Chidachi chimakhala ndi moyo wa miyezi 18 kuyambira tsiku lopangidwa. Sungani zida zomwe sizinagwiritsidwe ntchito pa kutentha kwa 2-30°C. MUSAZIYIKE MU MAFURI. Musagwiritse ntchito kupitirira tsiku lotha ntchito.
2. Musatsegule thumba lotsekedwa mpaka mutakonzeka kuchita mayeso, ndipo mayeso ogwiritsidwa ntchito kamodzi akulangizidwa kuti agwiritsidwe ntchito pamalo ofunikira (kutentha 2-35℃, chinyezi 40-90%) mkati mwa mphindi 60 mwachangu momwe mungathere.
3. Chitsanzo cha diluent chimagwiritsidwa ntchito nthawi yomweyo chikatsegulidwa.

MACHENJEZO NDI ZOCHENJEZERA
.Zida ziyenera kutsekedwa ndi kutetezedwa ku chinyezi.
Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina.
Zitsanzo zonse ziyenera kuonedwa ngati zowononga chilengedwe.
MUSAGWIRITSE NTCHITO reagent yotha ntchito.
. OSATSANITSANA ma reagent pakati pa zida zomwe zili ndi nambala yosiyana.
MUSAGWIRITSE NTCHITO makhadi oyesera ndi zinthu zina zilizonse zogwiritsidwa ntchito ngati zinyalala.
Kugwiritsa ntchito molakwika, kuchuluka kwa zitsanzo kapena zochepa kungayambitse kusintha kwa zotsatira.

LKUTSANZIRA
Monga momwe zimakhalira ndi mayeso aliwonse omwe amagwiritsa ntchito ma antibodies a mbewa, pali kuthekera kwa kusokonezedwa ndi ma antibodies a anti-mbewa a anthu (HAMA) mu chitsanzocho. Zitsanzo kuchokera kwa odwala omwe adalandira ma antibodies a monoclonal kuti apeze matenda kapena chithandizo zitha kukhala ndi HAMA. Zitsanzo zotere zingayambitse zotsatira zabodza kapena zabodza.
Zotsatira za mayesowa ndi za chithandizo chamankhwala chokha, siziyenera kukhala maziko okhawo odziwira matenda ndi chithandizo chamankhwala, kuyang'anira odwala kuyenera kuganiziridwa mokwanira pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwina kwa labotale, yankho la chithandizo, matenda a epidemiology ndi zina zambiri.
Chogwiritsiridwa ntchitochi chimagwiritsidwa ntchito poyesa seramu ndi plasma yokha. Sichingapeze zotsatira zolondola chikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina zotero.

MAKHALIDWE A NTCHITO

RMFUNDO ZOFUNIKA
1.Hansen JH, ndi ena. Kusokoneza kwa HAMA ndi Murine Monoclonal Antibody-Based Immunoassays [J].J wa Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Mtundu wa Ma Antibodies Osatha ndi Udindo mu Kusokoneza kwa Immunoassay [J].J wa Clin Immunoassay,1992,15:108-114.

Chinsinsi cha zizindikiro zomwe zagwiritsidwa ntchito:

	Chipangizo Chachipatala Chodziwitsira Matenda mu Vitro
	Wopanga
	Sungani pamalo otentha 2-30℃
	Tsiku lothera ntchito
	Musagwiritsenso ntchito
	CHENJEZO
	Onani Malangizo Ogwiritsira Ntchito

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