Agakoresho ko gusuzuma indwara ka Alpha-fetoprotein(isuzuma rya fluorescence immunochromatographic assay)
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE

Ikizamini cyo gusuzuma Alpha-fetoprotein (ikizamini cya fluorescence immunochromatographic assay) ni ikizamini cya fluorescence immunochromatographic assay cyo gupima Alpha-fetoprotein (AFP) mu maraso y'umuntu cyangwa mu maraso, gikoreshwa cyane cyane mu gusuzuma, kuvura no gupima kanseri y'umwijima. Ikizamini cyose cyagaragaye kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE

Poroteyine ya Alpha-feto (AFP) ni imwe mu zikoreshwa cyane mu kugaragaza kanseri. Ni glycoprotein ifite uburemere bwa molekile 70.000 n'isukari ya 4%. Ikorwa ahanini n'umwijima w'umwana uri mu nda, hagakurikiraho umuhondo w'umuhondo. Umwana uri mu nda yatangiye gukora mu gihe cy'ibyumweru 6, agera ku rwego rwo hejuru rw'ibyumweru 12 kugeza kuri 15, igipimo cya serum kiri hagati ya 1 na 3 g/L, n'amaraso y'inkondo y'umura avuka ari hagati ya 10 na 100 mg/L; imyaka 1 kugeza kuri 2 nyuma yo kuvuka kugeza ku rugero rw'umuntu mukuru; Gutwita bisanzwe bishobora kugera kuri 90 kugeza kuri 500 ng/mL hagati; Igipimo gisanzwe cya AFP mu maraso y'umuntu kiri hagati ya 2 na 8 ng/mL, ariko indwara nyinshi, cyane cyane umwijima, zigira ingaruka ku gipimo cya AFP.

IHAME RY'UBURYO BWAYO

Agace k'igikoresho cyo gupima gatwikiriwe na anti-AFP antibody ku gace k'igeragezwa naho anti-IgG antibody y'ihene anti-rabbit ku gace k'igenzura. Udupapuro two gupima dutwikiriwe na anti-AFP antibody twanditseho fluorescence na IgG y'urukwavu mbere y'igihe. Mu gusuzuma icyitegererezo cyagaragaje ko ari cyiza, anti-AFP antigen iri mu cyitegererezo ihuzwa na anti-AFP antibody yanditseho fluorescence, maze igakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'imikorere ya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo complex inyuze mu gace k'igeragezwa, ihujwe na anti-AFP coating antibody, ikora complex nshya. Urwego rwa AFP rufitanye isano ryiza n'ikimenyetso cya fluorescence, kandi ingano ya AFP mu cyitegererezo ishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw'umubiri rya fluorescence.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:

Ikarita yo gupima ku giti cyayo ipfunyitse mu gapfunyika gafite agakoresho ko gusukura 25T
.Ingero z'ibinyabutabire 25T
.Ipaki ishyirwamo 1

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe
ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
1. Ingero zapimwe zishobora kuba serum, heparin anticoagulant plasma cyangwa EDTA anticoagulant plasma.
2. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6.
3. Ingero zose zirinda gukonjesha no gushonga.

UBURYO BWO GUKORA IKIGANIRO
Mbere yo gupima, soma igitabo cy'amabwiriza agenga imikorere y'igikoresho n'agasanduku k'ibikoresho.
1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
4. Kura ikarita yo gupima mu gafuka k'ifishi.
5. Shyiramo ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
6. Ongeramo 20μL ya serum cyangwa plasma mu gipimo cy'icyitegererezo, hanyuma uvange neza.
7. Ongeraho umuti w'icyitegererezo wa 80μL ku iriba ry'ikarita.
8. Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 15, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
9. Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

INDANGAGACIRO ZITEGANYIJWE

AFP: <10ng/mL
Birasabwa ko buri laboratwari ishyiraho urwego rwayo rusanzwe rugaragaza umubare w’abarwayi bayo.

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO
.Amakuru yavuzwe haruguru ni ibyavuye mu isuzuma rya AFP reagent, kandi birasabwa ko buri laboratwari igomba gushyiraho ubwoko butandukanye bw'agaciro ko gupima AFP gakwiriye abaturage bo muri aka karere. Ibisubizo byavuzwe haruguru ni ibyo kwifashisha gusa.
Ibisubizo by'ubu buryo bireba gusa ingero z'ingero zashyizweho muri ubu buryo, kandi nta kugereranya kutaziguye n'ubundi buryo.
Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE
1. Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ikozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
2. Ntugafungure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
3. Icyitegererezo cy'umuti uvangwa n'amazi gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
.Isanduku igomba kuba ifunze kandi irinzwe ubushuhe.
Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
Ibipimo byose bigomba gufatwa nk'ibintu bishobora kwanduza.
NTUKORESHE reagent yarengeje igihe.
NTIBIRAHINDURANYE ibyuma bihinduranya ibikoresho hagati y'ibikoresho bifite ubwoko butandukanye No.
NTUGAKORESHE gukoresha amakarita yo gupima n'ibindi bikoresho byo gukoresha mu gihe cyo kuyakoresha.
.Gukoresha nabi, icyitegererezo gikabije cyangwa gito bishobora gutuma ibisubizo bihinduka.

LKWIGANA
Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwaba buhagaze, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima amaraso mu maraso no mu maraso. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.

IBIRANGA IMIKORERE

RIBIREBANA
1.Hansen JH, et al.HAMA Interference hamwe n'ibizamini by'imyuka bishingiye ku murine monoclonal antibody [J].J bya Clin Immunoassay, 1993,16:294-299.
2.Levinson SS. Imiterere y'uturemangingo tw'umubiri duto duto n'uruhare mu gutera ikibazo cy'ubudahangarwa bw'umubiri [J].J ya Clin Immunoassay, 1992,15:108-114.

Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha

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