Ikhithi yokuxilonga yeCardiac Troponin I(uvavanyo lwe-fluorescence immunochromatographic)
Ukusetyenziswa kokuxilongwa kwi-vitro kuphela
Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO
Ikhithi yokuxilonga yeCardiac Troponin I (uvavanyo lwe-fluorescence immunochromatographic) luvavanyo lwe-fluorescence immunochromatographic lokubona ubungakanani beCardiac Troponin I (cTnI) kwi-serum okanye kwi-plasma yomntu, isetyenziselwa ukuxilongwa kwe-AMI (Acute Myocardial Infarction). Zonke iisampulu ezilungileyo maziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zonyango kuphela.

ISISHWANKATHELO
Amanqanaba e-cTnI anyuke emva kweeyure ezimbalwa emva kokuba kwenzeke i-Myocardial infarction, afikelela kwinqanaba eliphezulu kwiiyure ezili-12-16, aza ahlala ephakamile emva kweentsuku ezi-4-9 emva kokuba kwenzeke i-Myocardial infarction. Inkcazo yehlabathi ye-myocardial infarction yesithathu ngo-2012: I-biomarker ekhethwayo-cTn(I okanye T), inobungcali obuphezulu bezicubu ze-myocardial kunye novakalelo oluphezulu lweklinikhi. Utshintsho kuxinzelelo lwe-cTn lubalulekile ekuchongweni kwe-AMI.

UMGAQO WENKQUBO
I-membrane yesixhobo sovavanyo igqunywe nge-anti-cTnI antibody kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula. I-Lable pad igqunywe nge-anti-cTnI antibody ebhalwe nge-fluorescence kunye ne-anti-cTnI antibody yomvundla kwangaphambili. Xa kuvavanywa isampuli echanekileyo, i-antigen ye-cTnI kwisampuli idibana ne-anti-cTnI antibody ebhalwe nge-fluorescence, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlula kwindawo yovavanyo, idibene ne-anti-cTnI coating antibody, yenza i-complex entsha. Inqanaba le-cTnI lihambelana kakuhle nesignali ye-fluorescence, kwaye uxinzelelo lwe-cTnI kwisampuli lunokufunyanwa ngovavanyo lwe-fluorescence immunoassay.

IZINTO EZINIKEZELWAYO NEZIXHOBO

Izixhobo zephakheji ze-25T：
Ikhadi lovavanyo lifakwe ifoyile nganye nganye ene-desiccant engama-25T
Iisampulu zokuxuba i-25T
Iphakheji efakiweyo 1

IZIXHOBO EZIFUNEKAYO KODWA AZINIKELWANGA
Isikhongozeli sokuqokelela isampuli, isibali-xesha

IMFUNDO YOKUQOKELELA NOKUGCINA IMIZEKELO
1.Iisampulu ezivavanyiweyo zinokuba yi-serum, i-heparin anticoagulant plasma okanye i-EDTA anticoagulant plasma.
2. Ngokweendlela eziqhelekileyo zokuqokelela isampuli. Isampuli ye-serum okanye yeplasma ingagcinwa efrijini kwi-2-8℃ iintsuku ezisi-7 kwaye igcinwe ngaphantsi kwe-15°C iinyanga ezi-6.
3.Zonke iisampuli ziphephe imijikelo yokuqandisa nokunyibilikisa.

INKQUBO YOVAVANYO
Nceda ufunde incwadi yemiyalelo yokusebenza kwesixhobo kunye nesihlomelo sephakheji ngaphambi kokuvavanya.
1. Beka ecaleni zonke ii-reagents kunye neesampuli ukuya kubushushu begumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokugqitha le-akhawunti ngokwendlela yokusebenza kwesixhobo, uze ufake i-interface yokubona.
3.Skena ikhowudi yokuchonga ukuze uqinisekise into yovavanyo.
4.Khupha ikhadi lovavanyo kwingxowa yefoyile.
5.Faka ikhadi lovavanyo kwindawo yokubeka ikhadi, skena ikhowudi ye-QR, uze umisele into yovavanyo.
6. Yongeza isampuli yeserum okanye iplasma engama-40μL kwisampuli ye-diluent, uze udibanise kakuhle.
7. Yongeza isisombululo sesampulu se-80μL kwisampulu yomthombo wekhadi.
8.Cofa iqhosha elithi “uvavanyo oluqhelekileyo”, emva kwemizuzu eli-15, isixhobo siza kuzibona ngokuzenzekelayo ikhadi lovavanyo, singafunda iziphumo kwisikrini sokubonisa isixhobo, size sirekhode/siprinte iziphumo zovavanyo.
9. Jonga imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

IINQOBO EZILINDELEKILEYO
i-cTnI <0.3ng/mL
Kucetyiswa ukuba ilabhoratri nganye imisele uluhlu lwayo oluqhelekileyo olumele inani lezigulana zayo.

IZIPHUMO ZOVAVANYO NOKUTOLIKWA
.Idatha engentla yimiphumo yovavanyo lwe-cTnI reagent, kwaye kucetyiswa ukuba ilabhoratri nganye imisele uluhlu lwamaxabiso okufumanisa i-cTnI afanelekileyo kuluntu olukulo mmandla. Iziphumo ezingentla zezokujonga kuphela.
Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi olusekwe kule ndlela, kwaye akukho kuthelekiswa ngokuthe ngqo nezinye iindlela.
Ezinye izinto zinokubangela iimpazamo kwiziphumo zokufumanisa, kuquka izizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOKUZINZISA
1. Le khithi ihlala iinyanga ezili-18 ukususela kumhla wokwenziwa kwayo. Gcina ezi khithi zingasetyenziswanga kwi-2-30°C. SUKUZIQANDA. Musa ukuzisebenzisa emva komhla wokuphelelwa.
2. Musa ukuvula isikhwama esivaliweyo ude ulungele ukwenza uvavanyo, kwaye uvavanyo olusetyenziswa kanye lucetyiswa ukuba lusetyenziswe phantsi kweemeko ezifunekayo (ubushushu obuyi-2-35℃, umswakama oyi-40-90%) kwimizuzu engama-60 ngokukhawuleza okukhulu.
3. Isampulu yomxube isetyenziswa kwangoko emva kokuba ivuliwe.

IZILUMKISO NEZILUMKISO
.Ikhithi kufuneka ivalwe kwaye ikhuselwe kumswakama.
Zonke iisampulu ezilungileyo ziya kuqinisekiswa ngezinye iindlela.
Zonke iisampulu maziphathwe njengezingcolisi ezinokubakho.
.MUSA ukusebenzisa i-reagent ephelelwe lixesha.
.MUSA ukutshintshanisa ii-reagents phakathi kweekiti ezinenani elahlukileyo.
.MUSA ukusebenzisa kwakhona amakhadi ovavanyo kunye nazo naziphi na izixhobo ezilahlwayo.
.Ukusetyenziswa gwenxa, isampuli egqithisileyo okanye encinci kunokubangela ukuphambuka kweziphumo.

LUKULINGANISA
.Njengakweyiphi na i-assay esebenzisa ii-antibodies zegundane, kunokwenzeka ukuba kubekho ukuphazamiseka kwee-antibodies ze-anti-mouse (HAMA) kwi-sampuli. Iisampuli ezivela kwizigulana ezifumene amalungiselelo e-monoclonal antibodies ukuze zixilongwe okanye zonyango zinokuba ne-HAMA. Ezi sampuli zinokubangela iziphumo ezilungileyo okanye ezimbi.
.Esi siphumo sovavanyo sesokubhekisela kwiklinikhi kuphela, akufuneki sisebenze njengesiseko sodwa sokuxilongwa kweklinikhi kunye nonyango, ulawulo lweklinikhi lwezigulane kufuneka luqwalaselwe ngokupheleleyo kunye neempawu zalo, imbali yezonyango, olunye uvavanyo lwelabhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi.
.Le reagent isetyenziswa kuphela kuvavanyo lwe-serum kunye ne-plasma. Isenokungafumani ziphumo zichanekileyo xa isetyenziswa kwezinye iisampuli ezifana namathe nomchamo njl.

IIMPAWU ZOKUSEBENZA

RIINGXELO

1.Hansen JH, et al. Ukuphazamiseka kwe-HAMA kwi-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Uhlobo lwee-Antibodies ze-Heterophilic kunye nendima ekuphazamisekeni kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Isitshixo seempawu ezisetyenzisiweyo:

	Isixhobo Sonyango Sokuxilonga se-In Vitro
	Umenzi
	Gcina kwi-2-30℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKISO
	Jonga Imiyalelo Yokusetyenziswa

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