Ikhithi Yokuxilonga ye-Alpha-fetoprotein(ukuhlolwa kwe-fluorescence immunochromatographic)
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi Yokuxilonga ye-Alpha-fetoprotein (i-fluorescence immunochromatographic assay) iyi-fluorescence immunochromatographic assay yokuthola inani le-Alpha-fetoprotein (AFP) ku-serum noma ku-plasma yomuntu, esetshenziswa kakhulu ekuxilongeni okusizayo, umphumela wokwelapha kanye nokubikezela kwe-carcinoma ye-hepatocellular eyinhloko. Zonke izibonelo ezinhle kumele ziqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

ISIFINYEZO

I-Alpha-fetoprotein (AFP) ingenye yezimpawu zomdlavuza ezisetshenziswa kakhulu. Iyi-glycoprotein enesisindo sama-molecule esingu-70,000 kanye noshukela ongu-4%. Ikhiqizwa kakhulu yisibindi sengane, ilandelwe yi-yolk sac. Ingane yaqala ukwenziwa amasonto ayi-6, ifinyelela inani eliphakeme lamasonto ayi-12 kuya kwangu-15, ukuhlushwa kwe-serum okungu-1 kuya ku-3 g/L, kanye negazi le-umbilical cord lapho izalwa elingu-10 kuya ku-100 mg/L; iminyaka engu-1 kuya kwemibili ngemva kokuzalwa kuze kube sezingeni lomuntu omdala; Ukukhulelwa okuvamile kungafinyelela ku-90 kuya ku-500 ng/mL phakathi; Okuqukethwe kwe-AFP kwe-serum yomuntu okuvamile kuphakathi kuka-2 no-8 ng/mL, kodwa izifo eziningi, ikakhulukazi i-hepatitis, zithinta inani le-AFP.

ISIMISO SENQUBO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-anti-AFP antibody endaweni yokuhlola kanye ne-anti-IgG antibody yembuzi endaweni yokulawula. Iphedi yelebula imbozwe nge-anti-AFP antibody ebhalwe nge-fluorescence kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-antigen ye-AFP kusampula ihlangana ne-anti-AFP antibody ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ohlangothini lwephepha elimuncayo, lapho i-complex idlula endaweni yokuhlola, ihlanganiswe ne-anti-AFP coating antibody, yakha i-complex entsha. Izinga le-AFP lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukuhlushwa kwe-AFP kusampula kungatholakala nge-fluorescence immunoassay assay.

IZINTO EZINGENAYO NEZINTO EZINIKEZWE

Izingxenye zephakheji ezingama-25T：

Ikhadi lokuhlola ngalinye lifakwe ifoyili ngesikhwama se-desiccant esingu-25T
.Izibonelo zokuxuba 25T
.Iphakheji yokufaka 1

KUDINGEKA IZINTO EZISETSHENZISWAYO KODWA AZINIKEZWA
Isitsha sokuqoqa isampula, isibali sikhathi
IQOQO LEZIBONELO NOKUGCINA
1. Amasampula ahlolwe angaba yi-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2. Ngokwezindlela ezijwayelekile zokuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku eziyi-7 kanye nokugcinwa ngaphansi kuka-15°C izinyanga eziyi-6.
3. Zonke izibonelo zigwema imijikelezo yokuqandisa nokuncibilikisa.

INQUBO YOKUHLOLA
Sicela ufunde incwadi yemiyalelo yokusebenza kwethuluzi kanye nephakheji ngaphambi kokuhlola.
1. Beka eceleni wonke ama-reagent namasampula ukuze izinga lokushisa legumbi lifinyelele ezingeni lokushisa legumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3.Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
6. Faka isampula ye-serum noma ye-plasma engu-20μL kusampula yokuxuba, bese uxuba kahle.
7. Engeza isisombululo sesampula esingu-80μL emthonjeni wesampula wekhadi.
8.Chofoza inkinobho ethi “ukuhlolwa okujwayelekile”, ngemva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
9.Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

AMANANI ALINDELEKILE

I-AFP: ＜10ng/mL
Kunconywa ukuthi ilabhorethri ngayinye isungule ububanzi bayo obujwayelekile obumele iziguli zayo.

IMIPHUMELA YOKUHLOLA NOKUHLUKANISWA
.Idatha engenhla ingumphumela wokuhlolwa kwe-AFP reagent, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule uhla lwamanani okuthola i-AFP afanele abantu bakulesi sifunda. Imiphumela engenhla iwukubhekisela kuphela.
.Imiphumela yale ndlela isebenza kuphela kumabanga okubhekisela asungulwe kule ndlela, futhi akukho ukuqhathaniswa okuqondile nezinye izindlela.
Ezinye izici zingadala amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza kanye nezinye izici zesampula.

ISITOKELO NOKUZINZIMA
1. Ikhithi ihlala izinyanga ezingu-18 kusukela ngosuku lokwenziwa kwayo. Gcina amakhithi angasetshenziswanga ku-2-30°C. UNGAWAQANDISI. Ungayisebenzisi ngemva kosuku lokuphelelwa yisikhathi.
2. Ungavuli isikhwama esivaliwe uze ulungele ukwenza isivivinyo, futhi isivivinyo sokusetshenziswa kanye sinconywa ukuthi sisetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa 2-35℃, umswakama 40-90%) zingakapheli imizuzu engama-60 ngokushesha okukhulu.
3. Isampula yokuxuba isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQAPHELO ZOKWENZA
.Ikhithi kufanele ivalwe futhi ivikelwe emswakama.
Zonke izibonelo ezinhle kufanele ziqinisekiswe ngezinye izindlela.
Zonke izibonelo kufanele ziphathwe njengezingcolisi ezingaba khona.
UNGASEBENZISI i-reagent ephelelwe yisikhathi.
AKUFANELE ukushintshaniswe ama-reagent phakathi kwamakhithi anenombolo ehlukile.
UNGASEBENZISI kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Ukusebenza kabi, isampula eningi kakhulu noma encane kungaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukuhlolwa okusebenzisa ama-antibodies egundane, kungenzeka ukuthi kukhona ukuphazamiseka kwama-antibodies e-anti-mouse (HAMA) esampula. Amasampula avela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal ukuze kutholakale isifo noma ukwelashwa angase abe ne-HAMA. Amasampula anjalo angabangela imiphumela emibi noma emibi engamanga.
.Lo mphumela wokuhlolwa ungowokubhekisela emtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kwezokwelapha kuphela, ukuphathwa kwezokwelapha kweziguli kufanele kucatshangelwe ngokuphelele kuhlanganiswe nezimpawu zaso, umlando wezokwelapha, okunye ukuhlolwa kwelabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi.
.Le reagent isetshenziswa kuphela ekuhlolweni kwe-serum kanye ne-plasma. Ingase ingatholi umphumela onembile uma isetshenziswa kwamanye amasampula afana namathe nomchamo njll.

IZIMALI ZOKUSEBENZA

RIZINKOMBA
1.Hansen JH, nabanye.Ukuphazamiseka kwe-HAMA nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Uhlobo Lwama-Antibodies Angama-Heterophilic kanye Nendima Ekuphazamisekeni Kwe-Immunoassay[J].J we-Clin Immunoassay,1992,15:108-114.

Isihluthulelo sezimpawu ezisetshenzisiwe:

	Idivayisi Yezokwelapha Yokuxilonga E-In Vitro
	Umkhiqizi
	Gcina ku-2-30℃
	Usuku lokuphelelwa isikhathi
	Ungasebenzisi Kabusha
	ISEXWAYISO
	Bheka Imiyalelo Yokusebenzisa

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