Kete Tātaritanga mō te Alpha-fetoprotein(whakamātautau ārai-ira-whakaahua-whakaahua)
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA

Ko te Kete Tātaritanga mō te Alpha-fetoprotein (whakamātautau immunochromatographic fluorescence) he whakamātautau immunochromatographic fluorescence mō te kimi i te nui o te Alpha-fetoprotein (AFP) i roto i te toto tangata, i te toto toto rānei, e whakamahia ana hei āwhina i te tātaritanga, hei whakaora i te mate pukupuku hepatocellular tuatahi. Me whakaū te tauira pai katoa mā ētahi atu tikanga. Ko tēnei whakamātautau he mea hanga mō te whakamahinga hauora anake.

WHAKARĀPOPOTONGA

Ko te Alpha-fetoprotein (AFP) tētahi o ngā tohu pukupuku e whakamahia whānuitia ana. He glycoprotein tēnei, he 70,000 te taumaha ngota, ā, he 4% te huka. Ko te ate o te kōpū te nuinga o te wā e hangaia ana, ā, muri iho ko te pūkohu toua. I tīmata te kōpū ki te hanga mō ngā wiki e 6, ka eke ki te tihi o te 12 ki te 15 wiki, ko te kukū toto o te 1 ki te 3 g/L, ā, ko te toto pito i te whānautanga he 10 ki te 100 mg/L; 1 ki te 2 tau i muri i te whānautanga ki te taumata pakeke; Ka taea e te haputanga noa te eke ki te 90 ki te 500 ng/mL i waenganui; Ko te ihirangi AFP toto tangata noa kei waenga i te 2 me te 8 ng/mL, engari he maha ngā mate, inā koa ko te hepatitis, e pā ana ki te uara AFP.

TE MĀTĀPONO O TE TIKANGA

Kua pania te kiriuhi o te taputapu whakamātautau ki te paturopi anti AFP i te rohe whakamātautau, me te paturopi anti rāpeti koati IgG i te rohe whakahaere. Kua pania te papa tapanga ki te paturopi anti AFP me te IgG rāpeti kua tohua ki te fluorescence i mua i te whakamātautau. I te whakamātautau i te tauira pai, ka honoa te antigen AFP i roto i te tauira ki te paturopi anti AFP kua tohua ki te fluorescence, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina paahitia e te matatini te rohe whakamātautau, ka honoa ki te paturopi paninga anti AFP, ka hanga he matatini hou. He hononga pai te taumata AFP ki te tohu fluorescence, ā, ka taea te kite i te kukū o te AFP i roto i te tauira mā te whakamātautau immunoassay fluorescence.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA

Ngā wāhanga mōkihi 25T：

Kāri whakamātautau takitahi he pepa konutea kua kapi ki te whakamaroke 25T
Ngā waiwhakarewa tauira 25T
.Whakaurunga mōkihi 1

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU
Ipu kohinga tauira, taima
TE KOHINGA TAUIRA ME TE ROKIROKI
1. Ko ngā tauira i whakamatautauria he toto toto, he toto toto heparin anticoagulant, he toto toto EDTA anticoagulant rānei.
2. E ai ki ngā tikanga paerewa, kohia he tauira. Ka taea te pupuri i te tauira toto, i te tauira plasma rānei ki roto i te pouaka whakamātao i te 2-8℃ mō ngā rā e 7, ā, ka taea te pupuri i te tauira i raro i te -15°C mō ngā marama e 6.
3. Kaua ngā tauira katoa e huri i te huka me te rewa.

TE TIKANGA WHAKAMĀTAUTAU
Pānuihia te pukapuka whakahaere taputapu me te tāpiringa ki te kete i mua i te whakamātautau.
1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2. Whakatuwheratia te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101), tāuruhia te kupuhipa takiuru pūkete e ai ki te tikanga whakahaere o te taputapu, ka tomo ki te atanga kimi.
3. Matawaihia te waehere whakaū niho hei whakaū i te tūemi whakamātautau.
4. Tangohia te kāri whakamātautau i te putea pepa konutea.
5. Whakauruhia te kāri whakamātautau ki roto i te mokamoka kāri, matawaihia te waehere QR, ka kimihia te tūemi whakamātautau.
6. Tāpirihia he 20μL o te tauira toto, te tauira plasma rānei ki te wai whakawera tauira, ā, whakaranua kia pai.
7. Tāpirihia he 80μL o te otinga tauira ki te puna tauira o te kāri.
8. Pāwhiritia te pātene "whakamātautau paerewa", i muri i te 15 meneti, ka kitea aunoa e te taputapu te kāri whakamātautau, ka taea te pānui i ngā hua mai i te mata whakaatu o te taputapu, ā, ka tuhia/tāia ngā hua whakamātautau.
9. Tirohia ngā tohutohu mō te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101).

NGĀ UARA E TŪMANAKOHIA ANA

AFP：<10ng/mL
E taunakihia ana kia whakatūhia e ia taiwhanga tōna ake awhe noa e tohu ana i te taupori tūroro.

NGĀ HUA WHAKAMĀTAUTAU ME TE WHAKAMĀRAMATANGA
Ko ngā raraunga o runga ake nei he hua o te whakamātautau matū AFP, ā, e kīia ana me whakatū e ia taiwhanga he whānuitanga o ngā uara kitenga AFP e tika ana mō te taupori o tēnei rohe. Hei tohutoro noa iho ngā hua o runga ake nei.
Ko ngā hua o tēnei tikanga ka pā anake ki ngā awhe tohutoro kua whakatūria i roto i tēnei tikanga, ā, kāore he whakataurite tika ki ētahi atu tikanga.
Ka taea hoki e ētahi atu āhuatanga te whakaputa hapa i ngā hua kimi, tae atu ki ngā take hangarau, ngā hapa whakahaere me ētahi atu āhuatanga tauira.

TE ROKIROKI ME TE PŪMAU
1. E 18 marama te roa o te oranga o te kete mai i te rā i hangaia ai. Penapenahia ngā kete kāore anō kia whakamahia ki te 2-30°C. KAUA E WHAKATIO. Kaua e whakamahia i tua atu i te rā paunga.
2. Kaua e huakina te putea hiri kia rite rā anō koe ki te mahi i tētahi whakamātautau, ā, e taunakihia ana kia whakamahia te whakamātautau kotahi-whakamahinga i raro i te taiao e hiahiatia ana (pāmahana 2-35℃, haumākū 40-90%) i roto i te 60 meneti i te wā tere rawa atu.
3. Ka whakamahia te wai whakamātao tauira i muri tonu i te whakatuwheratanga.

NGĀ WHAKATŪPATO ME NGĀ WHAKATŪPATO
Me hiri te kete, ā, me tiaki hoki i te makuku.
Me whakamana ngā tauira pai katoa mā ētahi atu tikanga.
Me whakaaro ngā tauira katoa he poke pea.
KAUA e whakamahia te matū kua pau te wā.
KAUA e whakawhiti i ngā tauhohenga i waenga i ngā kete me te nama rota rerekē.
KAUA e whakamahi anō i ngā kāri whakamātautau me ngā taputapu whakakorekore.
.Mēnā he te whakamahinga, he nui rawa, he iti rānei te tauira, ka rerekē pea ngā hua.

LWHAKATAURANGA
Pērā i ngā whakamātautau katoa e whakamahi ana i ngā paturopi kiore, tera pea ka pāngia te tauira e ngā paturopi ārai-kiore tangata (HAMA). Tērā pea kei roto i ngā tauira mai i ngā tūroro kua whiwhi i ngā whakaritenga o ngā paturopi monoclonal mō te tātaritanga, te rongoā rānei, te HAMA. Tērā pea ka puta he hua hē, he hua hē rānei.
Hei tohutoro haumanu anake tēnei hua whakamātautau, kaua e waiho hei pūtake anake mō te tātaritanga haumanu me te maimoatanga, me whai whakaaro whānui te whakahaere haumanu o ngā tūroro me ōna tohu, hītori hauora, ētahi atu whakamātautau taiwhanga, urupare maimoatanga, mate urutā me ētahi atu mōhiohio.
Ka whakamahia anake tēnei matū hei whakamātautau i te toto me te toto toto. Tērā pea kāore e tika te hua ina whakamahia mō ētahi atu tauira pēnei i te huware me te mimi me ētahi atu.

NGĀ ĀHUATANGA MAHI

RNGĀ WHAKAMĀRAMA
1.Hansen JH, et al. Te Whakararuraru HAMA ki ngā Immunoassays Murine Monoclonal Antibody-Based Immunoassays[J].J o Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Te Āhua o ngā Paturopi Heterophilic me te Tūranga i roto i te Whakararuraru Immunoassay[J].J o Clin Immunoassay,1992,15:108-114.

Ngā tohu matua i whakamahia:

	Pūrere Hauora Tātaritanga In Vitro
	Kaihanga
	Penapena ki te 2-30℃
	Rā Paunga
	Kaua e Whakamahi Anō
	WHAKATŪPATO
	Tirohia ngā Tohutohu Whakamahinga

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