KA'IDA

An shafa membrane na na'urar gwajin da maganin antigen na Rukunin A da adenovirus a yankin gwaji da kuma maganin antibody na IgG na akuya a yankin sarrafawa. Ana shafa murfin label da alamar fluorescence mai suna anti Group A da adenovirus da kuma maganin IgG na zomo a gaba. Lokacin gwada samfurin tabbatacce ga Rukunin A da adenovirus, Rukunin A da adenovirus a cikin samfurin suna haɗuwa da maganin fluorescence mai suna anti Rotavirus Group A da adenovirus, kuma suna samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, hadadden kwararar ruwa zuwa ga takardar sha. Lokacin da hadadden ya wuce yankin gwaji, ya haɗu da maganin anti-Rotavirus Group A da maganin antibody mai rufi na adenovirus, yana samar da sabon hadadden tsari. Idan ya kasance mara kyau, babu maganin Rotavirus Group A da maganin adenovirus a cikin samfurin, don haka ba za a iya samar da hadadden garkuwar jiki ba, ba za a sami layi ja a yankin ganowa ba (T). Ko akwai rotavirus na rukuni A da adenovirus a cikin samfurin, ana nuna IgG na linzamin kwamfuta mai lakabin latex zuwa yankin sarrafa inganci (C) kuma an kama shi da maganin rigakafi na IgG na akuya. Layin ja zai bayyana a yankin sarrafa inganci (C). Layin ja shine ma'aunin da ke bayyana a yankin sarrafa inganci (C) don tantance ko akwai isassun samfura da kuma ko tsarin chromatography na al'ada ne. Haka kuma ana amfani da shi azaman ma'aunin sarrafawa na ciki don reagents.

Tsarin Gwaji:

1. Buɗe murfin bututun tattara samfurin. Kada a zuba maganin a cikin kwalbar.
2. A cire sandar samfurin, a saka a cikin samfurin najasa (ko a yi amfani da sandar samfurin don ɗaukar kusan 50mg na najasa), sannan a mayar da sandar samfurin, a matse sosai sannan a girgiza sosai, a maimaita aikin sau 3. A ɗauki wani ɓangare daban na samfurin najasa a kowane lokaci. Bayan an ɗauki samfurin, a sanya sandar samfurin a cikin bututun tattara najasa wanda ke ɗauke da maganin ruwan, sannan a matse digon sosai. Idan najasar majiyyaci mai gudawa ta yi sirara, ana iya amfani da bambaro na filastik da za a iya zubarwa don ɗaukar samfurin. Ta amfani da samfurin pipette da za a iya zubarwa, a ɗauki samfurin najasa mai sirara daga mai gudawa, sannan a ƙara digo 3 (kimanin 100uL) a cikin bututun samfurin najasa.
3. A girgiza samfurin sosai sannan a cire murfin da ke kan gefen digo sannan a ajiye a gefe.
4. Idan aka adana a ƙaramin zafin jiki, ya kamata a mayar da kayan aikin zuwa zafin ɗaki kafin amfani. Cire katin gwaji daga jakar foil ɗin, a saka shi a kan teburin da aka daidaita sannan a yi masa alama.
5. Cire murfin daga bututun samfurin kuma jefar da digo biyu na farko na samfurin da aka narkar, ƙara digo 3 (kimanin 100uL) ba tare da kumfa ba a tsaye da hankali a cikin rijiyar samfurin na katin tare da dispette da aka bayar, fara lokacin.
6. Ya kamata a karanta sakamakon cikin mintuna 10-15, kuma ba shi da inganci bayan mintuna 15.