MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi Gulu A ndi antigen ya adenovirus pagawo loyesa ndi anti-Rabbit IgG antibody pachigawo chowongolera.Lable pad amakutidwa ndi fluorescence olembedwa anti Gulu A ndi adenovirus ndi kalulu IgG pasadakhale.Mukayesa zitsanzo zabwino za Gulu A ndi adenovirus, Gulu A ndi adenovirus mu zitsanzo zimaphatikizana ndi fluorescence yolembedwa kuti anti Rotavirus Gulu A ndi adenovirus, ndikupanga kusakanikirana kwa chitetezo.Pansi pa zochita za immunochromatography, zovuta kuyenda mu malangizo a absorbent pepala.Pamene zovuta zidadutsa gawo loyesa, zimaphatikizana ndi anti-Rotavirus Gulu A ndi anti-anti-antivirus ya adenovirus, zimapanga zovuta zatsopano.Ngati zili zoipa, palibe Rotavirus Gulu A ndi antigen ya adenovirus mu chitsanzo, kotero kuti zovuta za chitetezo cha mthupi zisapangidwe, sipadzakhala mzere wofiira pamalo ozindikira (T).Mosasamala kanthu kuti gulu A rotavirus ndi adenovirus zilipo mu chitsanzo, mbewa yolembedwa ndi latex IgG imajambulidwa kumalo olamulira bwino (C) ndikugwidwa ndi anti-mbewa IgG antibody.Mzere wofiira udzawonekera mu malo olamulira khalidwe (C).Mzere wofiyira ndi muyezo womwe umawonekera m'dera lowongolera (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yabwinobwino.Amagwiritsidwanso ntchito ngati muyezo wowongolera mkati mwa ma reagents.

Njira Yoyesera:

1. Tsegulani chipewa cha chubu chosonkhanitsira chitsanzo.Musatayire yankho mu botolo.
2. Chotsani ndodo, ndikuyika mu ndowe (kapena gwiritsani ntchito ndodo kuti mutenge pafupifupi 50mg ya ndowe), kenaka bwezerani ndodoyo, pukuta mwamphamvu ndikugwedezani bwino, bwerezani zomwezo katatu.Tengani gawo losiyana la ndowe nthawi iliyonse.Mukatha kuyesa, ikani ndodoyo mu chubu chotolera ndowe chomwe chili ndi chotsitsa chachitsanzocho, ndipo pukutani mwamphamvu chotsitsacho.Ngati ndowe za wodwala matenda otsekula m'mimba ndi zoonda, udzu wa pulasitiki wotayidwa ungagwiritsidwe ntchito potengera zitsanzo.Pogwiritsa ntchito zitsanzo za pipette zotayidwa tengani ndowe zoonda kwambiri za wodwala matenda otsekula m'mimba, kenaka onjezerani madontho atatu (pafupifupi 100uL) ku chubu chotengera ndowe.
3. Gwirani bwino chitsanzocho ndikuchotsani kapu pa nsonga yotsitsa ndikuyika pambali.
4. Mukasungidwa kutentha pang'ono, zidazo ziyenera kubwezeretsedwanso kutentha kusanagwiritse ntchito.Tulutsani khadi loyesera kuchokera mu thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikuyika chizindikiro.
5. Chotsani kapu ku chubu chitsanzo ndi kutaya madontho awiri oyambirira kuchepetsedwa chitsanzo, kuwonjezera 3 madontho (pafupifupi 100uL) palibe thovu kuchepetsedwa chitsanzo vertically ndi pang'onopang'ono mu chitsanzo chitsime cha khadi ndi dispette anapereka, kuyamba nthawi.
6. Zotsatira zake ziyenera kuwerengedwa mkati mwa mphindi 10-15, ndipo ndizosavomerezeka pakatha mphindi 15.