ỤKPỤRỤ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na antigen otu A na adenovirus na mpaghara nnwale ahụ na antibody IgG antibodies na mpaghara njikwa. A na-ekpuchi paadị label site na fluorescence nke akpọrọ anti Group A na adenovirus na oke bekee IgG tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma maka otu A na adenovirus, otu A na adenovirus dị na ihe nlele ahụ na-ejikọta ya na fluorescence nke akpọrọ anti Rotavirus Group A na adenovirus, ma na-emepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri. Mgbe ihe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na anti-Rotavirus Group A na antibody mkpuchi adenovirus, na-emepụta mgbagwoju ọhụrụ. Ọ bụrụ na ọ bụ ihe na-adịghị mma, enweghị Rotavirus Group A na antigen adenovirus na ihe nlele ahụ, nke mere na enweghị ike ịmepụta mgbagwoju anya mgbochi, agaghị enwe ahịrị uhie na mpaghara nchọpụta (T). N'agbanyeghị ma rotavirus na adenovirus dị na ihe nlele ahụ, a na-eji chromatograph òké a na-akpọ latex IgG mee ihe na mpaghara njikwa mma (C) ma jide ya site na mgbochi òké ewu IgG. Ahịrị uhie ga-apụta na mpaghara njikwa mma (C). Ahịrị uhie bụ ọkọlọtọ pụtara na mpaghara njikwa mma (C) iji chọpụta ma enwere ihe nlele zuru oke na ma usoro chromatography ọ bụ ihe nkịtị. A na-ejikwa ya dị ka ọkọlọtọ njikwa dị n'ime maka ihe ndị na-eme ihe.

Usoro Ule:

1. Mepee okpu nke ọkpọkọ nnakọta ihe nlele. Awụfula ihe ngwọta ahụ n'ime karama ahụ.
2. Wepụta mkpanaka nlele ahụ, tinye ya n'ime ihe nlele nsị ahụ (ma ọ bụ jiri mkpanaka nlele wepụta ihe dị ka 50mg nke nsị), wee tinyeghachi mkpanaka nlele ahụ, kpụchie ya nke ọma ma maa jijiji nke ọma, megharịa ihe ahụ ugboro atọ. Were akụkụ dị iche nke ihe nlele nsị ahụ oge ọ bụla. Mgbe ị sachara, tinye mkpanaka nlele ahụ n'ime ọkpọkọ nchịkọta nsị nke nwere ihe mgbakwunye ahụ, ma kpụchie ihe nlele ahụ nke ọma. Ọ bụrụ na nsị nke onye ọrịa nwere afọ ọsịsa dị gịrịgịrị, enwere ike iji ahịhịa plastik a na-atụfu atụfu mee ihe nlele. Site na iji ihe nlele pipette a na-atụfu atụfu, were ihe nlele nsị dị gịrịgịrị sitere n'aka onye ọrịa afọ ọsịsa, wee tinye ntapụ atọ (ihe dị ka 100uL) na ọkpọkọ nlele nsị.
3. Ma jijiji ihe nlele ahụ nke ọma ma wepụ mkpuchi dị n'ọnụ mmiri ahụ wee debe ya n'akụkụ.
4. Mgbe echekwara ya na obere okpomọkụ, a ga-eweghachi ngwa ahụ n'ọnọdụ okpomọkụ ụlọ tupu ejiri ya. Wepụ kaadị nnwale ahụ na akpa foil ahụ, tinye ya na tebụl dị larịị wee tinye akara na ya.
5. Wepụ mkpuchi ahụ n'ime ọkpọkọ ihe nlele ahụ ma tụfuo ntapụ abụọ mbụ nke ihe nlele ahụ a gwakọtara agwakọta, tinye ntapụ atọ (ihe dị ka 100uL) nke ihe nlele ahụ a gwakọtara agwakọta nke ọma ma jiri nwayọọ nwayọọ banye n'ime olulu ihe nlele nke kaadị ahụ yana ihe nlele enyere, malite oge.
6. A ga-agụ ihe a chọpụtara n'ime nkeji iri ruo iri na ise, ọ gaghịkwa adị irè mgbe nkeji iri na ise gachara.