Matenda zida za Estradiol(kuyesa kwa fluorescence immunochromatographic)
Kugwiritsa ntchito poyezetsa matenda m'thupi kokha
Chonde werengani bwino phukusili musanagwiritse ntchito ndipo tsatirani malangizo mosamala. Kudalirika kwa zotsatira za mayeso sikungatsimikizidwe ngati pali kusiyana kulikonse kuchokera ku malangizo omwe ali mu phukusili.

KUGWIRITSA NTCHITO KOFUNIKA
Chida Chodziwira Matenda cha Estradiol (kuyesa kwa immunochromatographic kwa kuwala) ndi njira yoyesera ya fluorescence immunochromatographic yodziwira kuchuluka kwa Estradiol (E2) mu seramu ya anthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka poyesa kuchuluka kwa Estradiol. Ndi reagent yothandizira kuzindikira matenda. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Mayesowa ndi a akatswiri azaumoyo okha.

CHIDULE
Estradiol (E2) ndi homoni yofunika kwambiri komanso yogwira ntchito kwambiri mu estrogen. Kulemera kwake kwa molekyulu ndi 272.3 D. Kawirikawiri, kwa amayi omwe si apakati, E2 imatulutsidwa makamaka ndi maselo a m'chimake ndi granular ndi maselo a luteal panthawi ya kukula kwa follicular. Pa nthawi ya pakati, E2 imatulutsidwa kwambiri ndi placenta, pomwe amuna amapangidwa kwambiri ndi machende. E2 ikalowa m'magazi, 1% mpaka 3% siimalumikizana ndi mapuloteni, 40% imalumikizana ndi hormone binding globulin (SHBG), ndipo ena amalumikizana ndi albumin, amasungunuka kuchokera ku chiwindi kukhala ma sulfates kapena gluconaldehyde esters, ndikutulutsidwa mu mkodzo. E2 ndi chizindikiro chofunikira cha homoni poyesa ntchito ya ovary.

MFUNDO YA NJIRAYI
Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi Estradiol pagawo loyesera ndi antibody ya mbuzi ya IgG yotsutsana ndi kalulu pagawo lolamulira. Mapepala olembera amakutidwa ndi antibody ya fluorescence yotsutsana ndi E2 ndi antibody ya kalulu IgG pasadakhale. Poyesa chitsanzo, E2 mu chitsanzo imaphatikizana ndi antibody ya fluorescence yodziwika ndi antibody ya E2, ndikupanga chisakanizo cha chitetezo chamthupi. Pansi pa ntchito ya immunochromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zadutsa gawo loyesera, chizindikiro cha fluorescent chaulere chidzaphatikizidwa ndi Estradiol pa nembanemba. Kuchuluka kwa Estradiol ndi mgwirizano woipa wa chizindikiro cha fluorescence, ndipo kuchuluka kwa Estradiol mu chitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.

ZOPANGIRA NDI ZIPANGIZO ZOPEREKEDWA

Zigawo za phukusi la 25T：
Khadi loyesera lokhala ndi foil payokha lokhala ndi desiccant 25T
Yankho la .A 25T
Yankho la .B 1
. Phukusi lolowera 1

Zipangizo zofunika koma siziperekedwa
Chidebe chosonkhanitsira zitsanzo, nthawi

Zitsanzo Zosonkhanitsira ndi Kusunga
1. Zitsanzo zomwe zayesedwa zitha kukhala seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2. Kutengera ndi njira zodziwika bwino, sonkhanitsani chitsanzo. Seramu kapena plasma chitsanzo chikhoza kusungidwa mufiriji pa 2-8℃ kwa masiku 7 ndikusungidwa pansi pa -15°C kwa miyezi 6.
Zitsanzo zonse kupewa kuzizira ndi kusungunula madzi.

NJIRA YOYESERA
Njira yoyesera chipangizochi onani buku la immunoanalyzer. Njira yoyesera reagent ndi iyi:
1. Ikani pambali zinthu zonse zoyeretsera ndi zitsanzo kutentha kwa chipinda.
2. Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi olowera mu akaunti malinga ndi njira yogwirira ntchito ya chidacho, ndikulowetsa mawonekedwe ozindikira.
3.Scan code yodziwitsa kuti mutsimikizire chinthu choyesera.
3. Tulutsani khadi loyesera m'thumba la foil.
4. Ikani khadi loyesera mu malo oikamo khadi, sikani QR code, ndikuzindikira chinthu choyesera.
5. Onjezani 30μL seramu kapena chitsanzo cha plasma mu yankho la A, ndipo sakanizani bwino.
6. Onjezani yankho la 20μL B ku chisakanizo chomwe chili pamwambapa, ndipo sakanizani bwino.
Siyani chisakanizocho kuti20mphindi.
Onjezani 80μL yosakaniza kuti muyesere chitsime cha khadi.
Dinani batani la "standard test", patatha mphindi 10, chipangizocho chidzazindikira khadi loyesera lokha, chimatha kuwerenga zotsatira kuchokera pazenera la chipangizocho, ndikulemba/kusindikiza zotsatira za mayeso.
Onani malangizo a Portable Immune Analyzer (WIZ-A101).

Zotsatira za Mayeso ndi Kutanthauzira

Deta yomwe ili pamwambapa ndi nthawi yowunikira yomwe idakhazikitsidwa kuti ipeze deta ya zida izi, ndipo akulangizidwa kuti labotale iliyonse ikhazikitse nthawi yowunikira kufunika kwachipatala kwa anthu m'derali.
Kuchuluka kwa Estradiol ndi kwakukulu kuposa kuchuluka kwa mankhwala, ndipo kusintha kwa thupi kapena kuyankha kwa kupsinjika kuyenera kuchotsedwa. Zoonadi, si zachilendo, ziyenera kuphatikiza kuzindikira zizindikiro zachipatala.
Zotsatira za njira iyi zimagwira ntchito kokha pa mndandanda wa zofotokozera zomwe zakhazikitsidwa ndi njira iyi, ndipo zotsatira zake sizingafanane mwachindunji ndi njira zina.
Zinthu zina zingayambitsenso zolakwika pakupeza zotsatira, kuphatikizapo zifukwa zaukadaulo, zolakwika pakugwira ntchito ndi zinthu zina zachitsanzo.

KUSUNGA NDI KUKHALA BWINO
1. Chidachi chimakhala ndi moyo wa miyezi 18 kuyambira tsiku lopangidwa. Sungani zida zomwe sizinagwiritsidwe ntchito pa kutentha kwa 2-30°C. MUSAZIYIKE MU MAFURI. Musagwiritse ntchito kupitirira tsiku lotha ntchito.
2. Musatsegule thumba lotsekedwa mpaka mutakonzeka kuchita mayeso, ndipo mayeso ogwiritsidwa ntchito kamodzi akulangizidwa kuti agwiritsidwe ntchito pamalo ofunikira (kutentha 2-35℃, chinyezi 40-90%) mkati mwa mphindi 60 mwachangu momwe mungathere.
3. Chitsanzo cha diluent chimagwiritsidwa ntchito nthawi yomweyo chikatsegulidwa.

MACHENJEZO NDI ZOCHENJEZERA
.Zida ziyenera kutsekedwa ndi kutetezedwa ku chinyezi.
Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina.
Zitsanzo zonse ziyenera kuonedwa ngati zowononga chilengedwe.
MUSAGWIRITSE NTCHITO reagent yotha ntchito.
MUSASINTHIRE ma reagent pakati pa zida zomwe zili ndi nambala yosiyana.
MUSAGWIRITSE NTCHITO makhadi oyesera ndi zinthu zina zilizonse zogwiritsidwa ntchito ngati zinyalala.
Kugwiritsa ntchito molakwika, kuchuluka kwa zitsanzo kapena zochepa kungayambitse kusintha kwa zotsatira.

LKUTSANZIRA
Monga momwe zimakhalira ndi mayeso aliwonse omwe amagwiritsa ntchito ma antibodies a mbewa, pali kuthekera kwa kusokonezedwa ndi ma antibodies a anti-mbewa a anthu (HAMA) mu chitsanzocho. Zitsanzo kuchokera kwa odwala omwe adalandira ma antibodies a monoclonal kuti apeze matenda kapena chithandizo zitha kukhala ndi HAMA. Zitsanzo zotere zingayambitse zotsatira zabodza kapena zabodza.
Zotsatira za mayesowa ndi za chithandizo chamankhwala chokha, siziyenera kukhala maziko okhawo odziwira matenda ndi chithandizo chamankhwala, chisamaliro cha odwala chiyenera kuganiziridwa mokwanira pamodzi ndi zizindikiro zake,
.mbiri ya zachipatala, kuyezetsa kwina kwa labotale, momwe chithandizo chimayankhidwira, matenda a epidemiology ndi zina zambiri.
Chogwiritsiridwa ntchitochi chimagwiritsidwa ntchito poyesa seramu ndi plasma yokha. Sichingapeze zotsatira zolondola chikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina zotero.
MAKHALIDWE A NTCHITO

RMFUNDO ZOFUNIKA
1.Hansen JH, ndi ena. Kusokoneza kwa HAMA ndi Murine Monoclonal Antibody-Based Immunoassays [J].J wa Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Mtundu wa Ma Antibodies Osatha ndi Udindo mu Kusokoneza kwa Immunoassay [J].J wa Clin Immunoassay,1992,15:108-114.

Chinsinsi cha zizindikiro zomwe zagwiritsidwa ntchito:

	Chipangizo Chachipatala Chodziwitsira Matenda mu Vitro
	Wopanga
	Sungani pamalo otentha 2-30℃
	Tsiku lothera ntchito
	Musagwiritsenso ntchito
	CHENJEZO
	Onani Malangizo Ogwiritsira Ntchito

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