Diagnostic Kit kwa Estradiol(fluorescence immunochromatographic assay)
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo. Kudalirika kwa zotsatira zoyeserera sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.

ZOFUNIKA KUGWIRITSA NTCHITO
Diagnostic Kit for Estradiol (fluorescence immunochromatographic assay) ndi fluorescence immunochromatographic assay pofuna kudziwa kuchuluka kwa Estradiol (E2) mu seramu yaumunthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka poyesa milingo ya Estradiol. Kuyezetsaku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.

CHIDULE
Estradiol (E2) ndi hormone yofunika kwambiri komanso yogwira ntchito kwambiri mu estrogen.Kulemera kwake kwa maselo ndi 272.3 D. Mwachizoloŵezi, kwa amayi omwe sali oyembekezera, E2 imatulutsidwa makamaka ndi sheath ndi maselo a granular ndi maselo a luteal panthawi ya chitukuko cha follicular. Amamanga ku mapuloteni, 40% amamanga ku mahomoni ogonana omwe amamanga globulin(SHBG),ndipo ena amamanga ku albumin, amatsuka kuchokera kuchiwindi kukhala ma sulfates osungunuka m'madzi kapena gluconaldehyde esters, ndi kuchotsedwa mumkodzo.

MFUNDO YA NTCHITO
Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi Estradiol pagawo loyesa ndi anti-Rabbit IgG antibody pachigawo chowongolera. Marker pad amakutidwa ndi fluorescence mark anti E2 antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo, E2 mu zitsanzo amaphatikiza ndi fluorescence yodziwika ndi anti E2 antibody, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, zovuta kuyenda mu njira ya pepala absorbent, pamene zovuta anadutsa mayesero dera, The free fulorosenti chikhomo adzakhala pamodzi ndi Estradiol pa nembanemba.

REAGENTS NDI Zipangizo ZOPEREKA

Zigawo za phukusi la 25T:
.Khadi loyesera payokha zojambulazo zopakidwa ndi desiccant 25T
.A yankho 25T
.B yankho 1
.Phukusi loyika 1

ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer

KUSONKHA ZITSANZO NDI KUSINTHA
1.Zitsanzo zoyesedwa zingakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2.Malinga ndi njira zoyenera sonkhanitsani zitsanzo. Seramu kapena madzi a m'magazi amatha kusungidwa mufiriji pa 2-8 ℃ kwa 7days ndi cryopreservation pansi -15 ° C kwa miyezi 6.
.Zitsanzo zonse zimapewa kuzizira kozizira.

NJIRA YOYENERA
Njira yoyesera ya chida onani buku la immunoanalyzer. Njira yoyeserera ya reagent ili motere
1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
3.Tulutsani khadi loyesera muthumba la zojambulazo.
4.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
5.Onjezani 30μL seramu kapena chitsanzo cha plasma ku A yankho, ndikusakaniza bwino.
6.Onjezani yankho la 20μL B kusakaniza pamwamba, ndikusakaniza bwino.
Siyani kusakaniza kwa20mphindi.
Onjezani kusakaniza kwa 80μL kuti muyese bwino khadi.
Dinani batani la "standard test", pakatha mphindi 10, chidacho chimangozindikira khadi yoyeserera, imatha kuwerenga zotsatira kuchokera pachiwonetsero cha chidacho, ndikulemba / kusindikiza zotsatira zoyeserera.
Onani malangizo a Portable Immune Analyzer(WIZ-A101).

ZOTSATIRA ZA MAYESE NDI KUMASULIRA

.Deta yomwe ili pamwambayi ndi nthawi yolozera yomwe yakhazikitsidwa kuti ipeze deta ya zidazi, ndipo akulangizidwa kuti labotale iliyonse iyenera kukhazikitsa nthawi yowonetsera kufunikira kwachipatala kwa anthu m'derali.
.Kuchulukira kwa Estradiol ndikwapamwamba kuposa momwe amafotokozera, ndipo kusintha kwa thupi kapena kupsinjika maganizo kuyenera kuchotsedwa.
.Zotsatira za njirayi zimangogwiritsidwa ntchito pazidziwitso zomwe zakhazikitsidwa ndi njirayi, ndipo zotsatira zake sizingafanane mwachindunji ndi njira zina.
.Zinthu zina zingayambitsenso zolakwika pazotsatira zodziwika, kuphatikizapo zifukwa zamakono, zolakwika zogwirira ntchito ndi zitsanzo zina.

KUSINTHA NDI KUKHALA
1. The zida ndi 18 miyezi alumali moyo kuyambira tsiku kupanga. Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C. OSATI MASIMA. Musagwiritse ntchito kupitirira tsiku lotha ntchito.
2.Musatsegule thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo kuyesa kamodzi kokha kumalangizidwa kuti agwiritsidwe ntchito pansi pa malo ofunikira (kutentha kwa 2-35 ℃, chinyezi 40-90%) mkati mwa 60 mins mwamsanga.
3.Sample diluent imagwiritsidwa ntchito mwamsanga mutangotsegulidwa.

CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga.
.MUSAMAGWIRITSE NTCHITO zomwe zidatha.
.OSATI kusinthanitsa ma reagents pakati pa zida zomwe zili ndi manambala osiyanasiyana.
.MUSAMAGWIRITSE NTCHITO makhadi oyesera ndi zina zilizonse zomwe zingathe kutayidwa.
.Misoperation, mochulukira kapena pang'ono zitsanzo kungayambitse zotsatira zopotoka.

LKUTSANZITSA
.Monga ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kuti kusokonezedwe ndi ma antibodies a anti-mbewa (HAMA) pachitsanzo. Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA. Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
.Zotsatira za mayesowa ndizongofotokozera zachipatala, siziyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, kasamalidwe ka odwala ayenera kuganiziridwa mozama kuphatikiza ndi zizindikiro zake,
.mbiri zachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, matenda a miliri ndi zina zambiri.
.Reagent iyi imagwiritsidwa ntchito poyezetsa seramu ndi plasma. Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.
ZINTHU ZOCHITIKA

RMALANGIZO
1.Hansen JH, ndi al.HAMA Kusokoneza ndi Murine Monoclonal Antibody-Based Immunoassays [J].J ya Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Chilengedwe cha Heterophilic Antibodies ndi Udindo mu Immunoassay Interference[J].J wa Clin Immunoassay,1992,15:108-114.

Chinsinsi cha zizindikiro zogwiritsidwa ntchito:

	In Vitro Diagnostic Medical Chipangizo
	Wopanga
	Sungani pa 2-30 ℃
	Tsiku lothera ntchito
	Osagwiritsanso Ntchito
	CHENJEZO
	Funsani Malangizo Ogwiritsa Ntchito

Xiamen Wiz Biotech CO., LTD
Address:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel: + 86-592-6808278
Fax: + 86-592-6808279