MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi gulu A ndi adenovirus antigen pamalo oyesera ndi mbuzi antibody ya IgG antibody yomwe ili pamalo olamulira. Mapepala olembera amakutidwa ndi fluorescence yolembedwa kuti anti Gulu A ndi adenovirus ndi kalulu IgG pasadakhale. Poyesa chitsanzo chabwino cha gulu A ndi adenovirus, gulu A ndi adenovirus yomwe ili mu chitsanzocho zimaphatikizana ndi fluorescence yolembedwa kuti anti Rotavirus Gulu A ndi adenovirus, ndikupanga chisakanizo cha chitetezo chamthupi. Pansi pa ntchito ya immunochromatography, zovuta zimapita molunjika ku pepala loyamwa. Pamene zovutazo zadutsa malo oyesera, zimaphatikizana ndi anti-Rotavirus Gulu A ndi adenovirus coating antibody, zimapanga chida chatsopano. Ngati zili zoipa, palibe Rotavirus Gulu A ndi adenovirus antigen yomwe ili mu chitsanzocho, kotero kuti chitetezo chamthupi sichingapangidwe, sipadzakhala mzere wofiira pamalo ozindikira (T). Kaya pali gulu la rotavirus ndi adenovirus mu sampuli, IgG ya mbewa yolembedwa latex imayikidwa mu chromatograph ku malo owongolera khalidwe (C) ndikugwidwa ndi antibody ya mbewa ya IgG. Mzere wofiira udzawonekera mu malo owongolera khalidwe (C). Mzere wofiira ndiye muyezo womwe umawonekera mu malo owongolera khalidwe (C) kuti udziwe ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yachibadwa. Imagwiritsidwanso ntchito ngati muyezo wamkati wa ma reagents.

Njira Yoyesera:

1. Tsegulani chivundikiro cha chubu chosonkhanitsira zitsanzo. Musatayire yankho m'botolo.
2. Tulutsani ndodo yoyesera, yomwe yaikidwa mu chitsanzo cha ndowe (kapena gwiritsani ntchito ndodo yoyesera kuti mutenge ndowe pafupifupi 50mg), kenako ikani ndodo yoyesera kumbuyo, pindani mwamphamvu ndikugwedeza bwino, bwerezani zomwezo katatu. Tengani gawo lina la chitsanzo cha ndowe nthawi iliyonse. Mukatha kutenga zitsanzo, ikani ndodo yoyesera mu chubu chosonkhanitsira ndowe chomwe chili ndi chosungunula cha chitsanzo, ndikukulunga chotsitsacho mwamphamvu. Ngati ndowe za wodwala wotsegula m'mimba ndi zopyapyala, udzu wapulasitiki wotayidwa ungagwiritsidwe ntchito kutenga zitsanzo. Pogwiritsa ntchito njira yoyesera ya pipette, tengani chitsanzo cha ndowe zopyapyala kuchokera kwa wodwala wotsegula m'mimba, kenako onjezerani madontho atatu (pafupifupi 100uL) mu chubu choyesera ndowe.
3. Gwedezani bwino chitsanzocho ndikuchotsa chivundikiro cha nsonga ya chotsitsa madzi kenako chiyikeni pambali.
4. Mukasunga kutentha kochepa, zida ziyenera kubwezeretsedwa kutentha kwa chipinda musanagwiritse ntchito. Tulutsani khadi loyesera kuchokera mu thumba la zojambulazo, liyikeni patebulo lofanana ndi tebulo ndikulilemba.
5. Chotsani chivundikirocho mu chubu cha chitsanzo ndikutaya madontho awiri oyamba a chitsanzo chochepetsedwa, onjezerani madontho atatu (pafupifupi 100uL) opanda thovu lochepetsedwa molunjika komanso pang'onopang'ono mu chitsime cha chitsanzo cha khadi ndi dispette yoperekedwa, yambani nthawi.
6. Zotsatira zake ziyenera kuwerengedwa mkati mwa mphindi 10-15, ndipo sizigwira ntchito patatha mphindi 15.