IHAME

Agace k'igikoresho cyo gupima gatwikiriwe n'itsinda rya A na antigen ya adenovirus ku gace k'igeragezwa, naho antibody ya IgG ya antigen y'ihene irwanya inkwavu ku gace k'igenzura. Agapapuro k'isuzuma gatwikiriwe n'itsinda rya A na adenovirus na IgG y'inkwavu mbere y'igihe. Mu gihe cyo gupima icyitegererezo cyagaragaye ko ari Itsinda rya A na adenovirus, Itsinda rya A na adenovirus mu gipimo bihurira hamwe na antibody ya Rotavirus Itsinda rya A na adenovirus, maze bigakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'imikorere ya immunochromatography, urusobe rw'imitsi runyura mu cyerekezo cy'impapuro zifata. Iyo urusobe rwanyuze mu gace k'igerageza, rwahujwe na antibody ya Rotavirus Itsinda rya A na antibody ya adenovirus, rukora urusobe rushya. Iyo rudafite negative, nta antigen ya Rotavirus Itsinda rya A na adenovirus mu gipimo, bityo imiterere y'ubudahangarwa bw'umubiri idashobora kubaho, nta murongo utukura uzaba uhari mu gace k'isuzuma (T). Nubwo muri iyo ngero haba harimo agatsiko ka rotavirus na adenovirus byo mu itsinda A, IgG y'imbeba ifite latex ishyirwa kuri chromatographe mu gace kagenzura ubuziranenge (C) hanyuma igafatwa na antibody ya IgG yo mu mbeba irwanya imbeba. Umurongo utukura uzagaragara mu gace kagenzura ubuziranenge (C). Umurongo utukura ni wo ugaragara mu gace kagenzura ubuziranenge (C) kugira ngo harebwe niba hari ingero zihagije kandi niba inzira ya chromatography ari isanzwe. Ikoreshwa kandi nk'igipimo ngenderwaho cy'igenzura ry'imbere ku binyabutabire.

Uburyo bwo gupima:

1. Fungura umupfundikizo w'agacupa ko gukusanya ingero. Ntugasuke umuti mu icupa.
2. Kuramo agati k'ingero, kashyizwe mu gipimo cy'umwanda (cyangwa koresha agati k'ingero kugira ngo ufate hafi 50mg y'umwanda), hanyuma usubize agati k'ingero inyuma, ukaze neza hanyuma uzunguze neza, ongeramo icyo gikorwa inshuro 3. Fata igice gitandukanye cy'igipimo cy'umwanda buri gihe. Nyuma yo gufata ingero, shyira agati k'ingero mu muyoboro wo gukusanya umwanda urimo umuti w'ingero, hanyuma ukaze neza agati k'ingero. Niba umwanda w'umurwayi ufite impiswi woroshye, ushobora gukoresha agati ka pulasitiki gakoreshwa mu gufata ingero. Ukoresheje ingero z'imiyoboro ikoreshwa mu gufata ingero z'umwanda woroshye ku murwayi w'impiswi, hanyuma wongereho ibitonyanga 3 (hafi 100uL) mu muyoboro w'umwanda.
3. Kungura neza icyitegererezo hanyuma ukureho agapfundikizo ku mutwe w'agatonyanga hanyuma ushyire ku ruhande.
4. Iyo bibitswe ku bushyuhe buke, agakoresho kagomba kugasubizwa ku bushyuhe bw'icyumba mbere yo kubikoresha. Kura ikarita y'ikizamini mu gafuka k'ifi, uyishyire ku meza iringaniye hanyuma uyishyireho ikimenyetso.
5. Kuraho umupfundikizo mu muyoboro w'icyitegererezo hanyuma ujugunye ibitonyanga bibiri bya mbere byakuwemo amazi, ongeramo ibitonyanga 3 (hafi 100uL) nta bitonyanga byakuwemo amazi bihagaze buhoro buhoro mu mwobo w'ikarita ufite dispette yatanzwe, tangira igihe.
6. Ibisubizo bigomba gusomwa mu minota 10-15, kandi nyuma y'iminota 15 nta gaciro bifite.