NHEYO

Kamudzira kemudziyo wekuyedza kakaputirwa neboka A ne adenovirus antigen panzvimbo yekuyedza uye mbudzi anti rabbit IgG antibody panzvimbo yekudzora. Mapepa eLable pad akaputirwa ne fluorescence yakanyorwa anti anti Group A ne adenovirus ne rabbit IgG pachine nguva. Pakuyedza positive sampuro yeboka A ne adenovirus, boka A ne adenovirus mumuenzaniso zvinosangana ne fluorescence yakanyorwa anti anti Rotavirus Group A ne adenovirus, uye zvinoumba immune mixture. Pasi pekushanda kwe immunochromatography, complex inoyerera ichienda kune bepa rinonyudza. Kana complex yapfuura nzvimbo yekuyedza, yakasanganiswa ne anti-Rotavirus Group A ne adenovirus coating antibody, inoumba complex itsva. Kana iri negative, hapana Rotavirus Group A ne adenovirus antigen mumuenzaniso, saka immune complexes hadzigone kuumbwa, hapazovi ne red line munzvimbo yekuonekwa (T). Pasinei nekuti boka re rotavirus ne adenovirus zviri muboka A here, mbeva inonzi latex-labeled IgG inotorwa kubva ku chromatograph kuenda kunzvimbo yekudzora hunhu (C) uye inobatwa ne mbudzi anti-mouse IgG antibody. Mutsetse mutsvuku uchaonekwa munzvimbo yekudzora hunhu (C). Mutsetse mutsvuku ndiwo mwero unoonekwa munzvimbo yekudzora hunhu (C) wekuona kana paine sampuli dzakakwana uye kana maitiro e chromatography ari akajairwa. Inoshandiswawo semwero wekudzora mukati mema reagents.

Maitiro Ekuyedza:

1. Vhura chivharo chechubhu yekuunganidzira sampuro. Usadururire mvura mubhodhoro.
2. Bvisa chimuti chekuyedza, chakaiswa mumuenzaniso wetsvina (kana kushandisa chimuti chekuyedza kuti utore inenge 50mg yetsvina), wobva wadzosera chimuti chekuyedza, wochisunga wozunza zvakanaka, wodzokorora chiito katatu. Tora chikamu chakasiyana chemuenzaniso wetsvina nguva imwe neimwe. Mushure mekuyedza, isa chimuti chekuyedza muchubhu yekuunganidzira tsvina ine musanganiswa wemuenzaniso, wobva wasunga chidhonzero zvakasimba. Kana tsvina yemurwere ane manyoka yakatetepa, jira repurasitiki rinoraswa rinogona kushandiswa kuyera. Uchishandisa kuyera pipette inoraswa, tora sampuli yetsvina yakatetepa kubva kumurwere ane manyoka, wobva wawedzera madonhwe matatu (anenge 100uL) muchubhu yekuyedza tsvina.
3. Zunzai zvakanaka muenzaniso mobvisa chivharo chiri pamucheto wedonhedzo wobva waisa parutivi.
4. Kana yachengetwa patembiricha yakaderera, kit inofanira kudzorerwa patembiricha yemukamuri usati washandisa. Bvisa kadhi rekuyedza kubva mubhegi refoil, riise patafura yakaenzana worimaka.
5. Bvisa chivharo kubva pachubhu yemuenzaniso worasa madonhwe maviri ekutanga akasanganiswa, wedzera madonhwe matatu (anenge 100uL) pasina bubble yakasanganiswa yakatwasuka uye zvishoma nezvishoma mutsime remuenzaniso wekadhi rine dispette yakapihwa, tanga nguva.
6. Mhedzisiro yacho inofanira kuverengwa mukati memaminitsi gumi kusvika gumi nemashanu, uye haichashandi mushure memaminitsi gumi nemashanu.