Ikhithi yokuxilonga ye-Estradiol(uvavanyo lwe-fluorescence immunochromatographic)
Ukusetyenziswa kokuxilongwa kwi-vitro kuphela
Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO
Isixhobo sokuxilonga se-Estradiol (uvavanyo lwe-fluorescence immunochromatographic) luvavanyo lwe-fluorescence immunochromatographic lokufumanisa ubungakanani be-Estradiol (E2) kwi-serum okanye kwi-plasma yomntu, esetyenziswa kakhulu ukuvavanya amanqanaba e-Estradiol. Sisixhobo sokuncedisa ukuxilongwa. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zonyango kuphela.

ISISHWANKATHELO
I-Estradiol (E2) yeyona hormone ibalulekileyo nesebenzayo kwi-estrogen. Ubunzima bayo bemolekyuli yi-272.3 D. Ngokubanzi, kubafazi abangakhulelwanga, i-E2 ikhutshwa kakhulu ziiseli zesheath kunye neegranular kunye neeseli zeluteal ngexesha lophuhliso lwe-follicular. Ngexesha lokukhulelwa, i-E2 ikhutshwa kakhulu yi-placenta, ngelixa amadoda eveliswa kakhulu zii-testicles. Emva kokuba i-E2 ingene egazini, i-1% ukuya kwi-3% ayibopheleleki kwiiproteni, i-40% ibopheleleka kwi-sex hormone binding globulin (SHBG), kwaye ezinye zibopheleleka kwi-albumin, zinyibilike ukusuka esibindini ziye kwii-sulfates ezinyibilikayo emanzini okanye ii-esters ze-gluconaldehyde, kwaye zikhutshwe kumchamo. I-E2 sisalathisi esibalulekileyo se-hormone sokuvavanya ukusebenza kwe-ovarian.

UMGAQO WENKQUBO
I-membrane yesixhobo sovavanyo igqunywe nge-conjugate ye-BSA kunye ne-Estradiol kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yokulawula. I-Marker pad igqunywe nge-antibody ye-E2 uphawu lwe-fluorescence kunye ne-IgG yerabbit yomvundla kwangaphambili. Xa kuvavanywa isampuli, i-E2 kwisampuli idityaniswa ne-antibody ye-E2 uphawu lwe-fluorescence, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlule kwindawo yovavanyo, uphawu lwe-fluorescent olukhululekileyo luya kudityaniswa ne-Estradiol kwi-membrane. Uxinzelelo lwe-Estradiol luhambelana kakubi nomqondiso we-fluorescence, kwaye uxinzelelo lwe-Estradiol kwisampuli lunokubonwa ngovavanyo lwe-immunoassay lwe-fluorescence.

IZINTO EZINIKEZELWAYO NEZIXHOBO

Izixhobo zephakheji ze-25T：
Ikhadi lovavanyo ngalinye lifakwe ifoyile ngesixhobo sokucoca i-desiccant esingu-25T
Isisombululo se-.A 25T
Isisombululo se-.B 1
.Iphakheji yokufaka 1

IZIXHOBO EZIFUNEKAYO KODWA AZINIKELWANGA
Isikhongozeli sokuqokelela isampuli, isibali-xesha

IMFUNDO YOKUQOKELELA NOKUGCINA IMIZEKELO
1.Iisampulu ezivavanyiweyo zinokuba yi-serum, i-heparin anticoagulant plasma okanye i-EDTA anticoagulant plasma.
2. Ngokweendlela eziqhelekileyo zokuqokelela isampuli. Isampuli ye-serum okanye yeplasma ingagcinwa efrijini kwi-2-8℃ iintsuku ezisi-7 kwaye igcinwe ngaphantsi kwe-15°C iinyanga ezi-6.
Zonke iisampuli ziphepha imijikelo yokuqandisa nokunyibilikisa.

INKQUBO YOVAVANYO
Inkqubo yovavanyo lwesixhobo jonga incwadi yemiyalelo ye-immunoanalyzer. Inkqubo yovavanyo lwe-reagent yile ilandelayo
1. Beka ecaleni zonke ii-reagents kunye neesampuli ukuya kubushushu begumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokugqitha le-akhawunti ngokwendlela yokusebenza kwesixhobo, uze ufake i-interface yokubona.
3.Skena ikhowudi yokuchonga ukuze uqinisekise into yovavanyo.
3.Khupha ikhadi lovavanyo kwingxowa yefoyile.
4.Faka ikhadi lovavanyo kwindawo yokubeka ikhadi, skena ikhowudi ye-QR, uze umisele into yovavanyo.
5. Yongeza isampuli yeserum okanye iplasma engama-30μL kwisisombululo se-A, uze udibanise kakuhle.
6. Yongeza isisombululo se-20μL B kumxube ongentla, uze udibanise kakuhle.
Shiya umxube ukuze20imizuzu.
Yongeza umxube we-80μL ukuze ufumane isampuli yequla lekhadi.
Cofa iqhosha elithi “uvavanyo oluqhelekileyo”, emva kwemizuzu eli-10, isixhobo siza kuzibona ngokuzenzekelayo ikhadi lovavanyo, sifunde iziphumo kwisikrini sokubonisa isixhobo, size sirekhode/siprinte iziphumo zovavanyo.
Jonga imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

IZIPHUMO ZOVAVANYO NOKUTOLIKWA

.Idatha engentla lixesha elimiselweyo lokufumana idatha yale khithi, kwaye kucetyiswa ukuba ilabhoratri nganye imisele ixesha elimiselweyo lokubaluleka kweklinikhi okufanelekileyo kubemi abakulo mmandla.
Uxinzelelo lwe-Estradiol luphezulu kunoluhlu olufunekayo, kwaye utshintsho lwe-physiological okanye impendulo yoxinzelelo kufuneka lungabandakanywa. Enyanisweni, okungaqhelekanga, kufuneka kudibaniswe ukuxilongwa kweempawu zeklinikhi.
Iziphumo zale ndlela zisebenza kuphela kuluhlu lwesalathiso olumiselwe yile ndlela, kwaye iziphumo azifani ngokuthe ngqo nezinye iindlela.
Ezinye izinto zinokubangela iimpazamo kwiziphumo zokufumanisa, kuquka izizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOKUZINZISA
1. Le khithi ihlala iinyanga ezili-18 ukususela kumhla wokwenziwa kwayo. Gcina ezi khithi zingasetyenziswanga kwi-2-30°C. SUKUZIQANDA. Musa ukuzisebenzisa emva komhla wokuphelelwa.
2. Musa ukuvula isikhwama esivaliweyo ude ulungele ukwenza uvavanyo, kwaye uvavanyo olusetyenziswa kanye lucetyiswa ukuba lusetyenziswe phantsi kweemeko ezifunekayo (ubushushu obuyi-2-35℃, umswakama oyi-40-90%) kwimizuzu engama-60 ngokukhawuleza okukhulu.
3. Isampulu yomxube isetyenziswa kwangoko emva kokuba ivuliwe.

IZILUMKISO NEZILUMKISO
.Ikhithi kufuneka ivalwe kwaye ikhuselwe kumswakama.
Zonke iisampulu ezilungileyo maziqinisekiswe ngezinye iindlela.
Zonke iisampulu maziphathwe njengezingcolisi ezinokubakho.
.MUSA ukusebenzisa i-reagent ephelelwe lixesha.
.MUSA ukutshintshanisa ii-reagents phakathi kweekiti ezinenani elahlukileyo..
.MUSA ukusebenzisa kwakhona amakhadi ovavanyo kunye nazo naziphi na izixhobo ezilahlwayo.
.Ukusetyenziswa gwenxa, isampuli egqithisileyo okanye encinci kunokubangela ukuphambuka kweziphumo.

LUKULINGANISA
.Njengakweyiphi na i-assay esebenzisa ii-antibodies zegundane, kunokwenzeka ukuba kubekho ukuphazamiseka kwee-antibodies ze-anti-mouse (HAMA) kwi-sampuli. Iisampuli ezivela kwizigulana ezifumene amalungiselelo e-monoclonal antibodies ukuze zixilongwe okanye zonyango zinokuba ne-HAMA. Ezi sampuli zinokubangela iziphumo ezilungileyo okanye ezimbi.
.Esi siphumo sovavanyo sesokubhekisela kwiklinikhi kuphela, akufuneki sisebenze njengesiseko sodwa sokuxilongwa kweklinikhi kunye nonyango, ulawulo lweklinikhi lwezigulane kufuneka luqwalaselwe ngokupheleleyo kunye neempawu zalo,
.imbali yezonyango, olunye uvavanyo lwelabhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi.
.Le reagent isetyenziswa kuphela kuvavanyo lwe-serum kunye ne-plasma. Isenokungafumani ziphumo zichanekileyo xa isetyenziswa kwezinye iisampuli ezifana namathe nomchamo njl.
IIMPAWU ZOKUSEBENZA

RIINGXELO
1.Hansen JH, et al. Ukuphazamiseka kwe-HAMA kwi-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Uhlobo lwee-Antibodies ze-Heterophilic kunye nendima ekuphazamisekeni kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Isitshixo seempawu ezisetyenzisiweyo:

	Isixhobo Sonyango Sokuxilonga se-In Vitro
	Umenzi
	Gcina kwi-2-30℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKISO
	Jonga Imiyalelo Yokusetyenziswa

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