Kit ɗin Bincike don Estradiol(gwajin immunochromatographic na fluorescence)
Don amfani da ganewar asali kawai a cikin vitro
Da fatan za a karanta wannan fakitin da aka saka a hankali kafin amfani kuma a bi umarnin sosai. Ba za a iya tabbatar da ingancin sakamakon gwaji ba idan akwai wata matsala daga umarnin da ke cikin wannan fakitin.

AN YI NIYYA DA AMFANI
Kayan Bincike na Estradiol (gwajin fluorescence immunochromatographic) gwajin fluorescence immunochromatographic ne don gano adadi na Estradiol (E2) a cikin jini ko plasma na ɗan adam, wanda galibi ana amfani da shi don tantance matakan Estradiol. Wani reagent ne na ganewar asali na taimako. Dole ne a tabbatar da duk samfuran da ke da kyau ta wasu hanyoyin. Wannan gwajin an yi shi ne don amfanin ƙwararru a fannin kiwon lafiya kawai.

TAƘAITACCE
Estradiol (E2) shine hormone mafi mahimmanci kuma mafi aiki a cikin estrogen. Nauyin kwayoyin halittarsa ​​shine 272.3 D. Gabaɗaya, ga mata masu juna biyu, E2 galibi ana fitar da shi ta ƙwayoyin sel da granular da ƙwayoyin luteal yayin haɓakar follicular. A lokacin daukar ciki, E2 galibi ana fitar da shi ta hanyar mahaifa, yayin da maza galibi ana samar da su ta hanyar ƙwai. Bayan E2 ya shiga jini, kashi 1 zuwa 3% ba sa ɗaurewa da furotin, kashi 40% suna ɗaurewa da globulin jima'i mai ɗaurewa (SHBG), wasu kuma suna ɗaurewa da albumin, suna narkewa daga hanta zuwa sulfates masu narkewa a ruwa ko gluconaldehyde esters, kuma suna fitarwa daga fitsari. E2 muhimmin ma'aunin hormone ne don kimanta aikin ovarian.

KA'IDUN TSARIN
An rufe membrane na na'urar gwajin da haɗin BSA da Estradiol a yankin gwaji da kuma antibody na IgG na akuya a yankin sarrafawa. Ana shafa faifan alamar da alamar haske ta antibody na E2 da IgG na zomo a gaba. Lokacin gwada samfurin, E2 a cikin samfurin yana haɗuwa da antibody na E2 mai alamar haske, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun a cikin jagorancin takardar sha, lokacin da hadaddun ya wuce yankin gwaji, za a haɗa alamar fluorescent kyauta tare da Estradiol akan membrane. Yawan Estradiol yana da alaƙa mara kyau ga siginar fluorescent, kuma yawan Estradiol a cikin samfurin ana iya gano shi ta hanyar gwajin immunoassay na fluorescence.

MASU YIN REAGENTS DA KAYAN DA AKA KAWO

Kayan aikin fakitin 25T:
.Katin gwaji daban-daban an saka shi a cikin jaka mai ɗauke da ruwan zafi 25T
.Mafita 25T
Maganin .B 1
.Saka fakiti 1

ANA BUKATAR KAYAN AIKI AMMA BA A BA DA SU BA
Samfurin tarin akwati, mai ƙidayar lokaci

TARIN SAMFURI DA AJIYEWA
1. Samfuran da aka gwada na iya zama na jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2. Dangane da dabarun da aka saba amfani da su, ana iya ajiye samfurin jini ko na plasma a cikin firiji a zafin 2-8℃ na tsawon kwanaki 7, kuma ana iya ajiye shi a wuri mai sanyi a ƙasa da -15°C na tsawon watanni 6.
.Duk samfuran suna guje wa zagayowar daskarewa da narkewa.

Tsarin Gwaji
Tsarin gwajin kayan aikin duba littafin immunoanalyzer. Tsarin gwajin reagent shine kamar haka:
1. A ajiye dukkan sinadaran da aka yi amfani da su da kuma samfuran da aka yi amfani da su a zafin ɗaki.
2. Buɗe Mai Nazarin Jiki Mai Ɗaukewa (WIZ-A101), shigar da kalmar sirri ta asusun bisa ga hanyar aiki ta kayan aikin, sannan ka shigar da hanyar ganowa.
3. Duba lambar haƙoran don tabbatar da abin gwajin.
3. Cire katin gwaji daga jakar foil.
4. Saka katin gwaji a cikin ramin katin, duba lambar QR, sannan ka tantance abin gwajin.
5. Ƙara samfurin serum ko plasma na 30μL a cikin maganin A, sannan a gauraya sosai.
6. A zuba maganin B mai nauyin 20μL a cikin hadin da ke sama, sannan a gauraya sosai.
Bar cakuda don20mintuna.
Ƙara cakuda 80μL don yin samfurin rijiyar katin.
Danna maɓallin "gwaji na yau da kullun", bayan mintuna 10, kayan aikin zai gano katin gwaji ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aikin, kuma ya yi rikodin/buga sakamakon gwajin.
Duba umarnin Portable Immune Analyzer (WIZ-A101).

SAKAMAKON GWAJI DA FASSARAR

. Bayanan da ke sama sune tazarar da aka kafa don bayanan gano wannan kayan aiki, kuma ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kamata ya kafa tazarar da ta dace don mahimmancin asibiti na yawan jama'a a wannan yanki.
. Yawan sinadarin Estradiol ya fi na ma'aunin tunani, kuma ya kamata a cire canje-canje a fannin jiki ko kuma martanin damuwa. Hakika, ya kamata a haɗa ganewar alamun cutar a asibiti.
.Sakamakon wannan hanyar yana aiki ne kawai ga kewayon tunani da wannan hanyar ta kafa, kuma sakamakon ba ya kama da na sauran hanyoyi kai tsaye.
.Wasu dalilai kuma na iya haifar da kurakurai a sakamakon ganowa, gami da dalilan fasaha, kurakuran aiki da sauran abubuwan samfurin.

Ajiya da kwanciyar hankali
1. Kayan aikin yana da tsawon watanni 18 daga ranar da aka ƙera shi. Ajiye kayan da ba a yi amfani da su ba a zafin 2-30°C. KAR A DASKARE. Kada a yi amfani da su fiye da ranar karewa.
2. Kar a buɗe jakar da aka rufe har sai kun shirya yin gwaji, kuma ana ba da shawarar a yi amfani da gwajin amfani da shi sau ɗaya a ƙarƙashin yanayin da ake buƙata (zafin jiki 2-35℃, danshi 40-90%) cikin mintuna 60 da sauri.
3. Ana amfani da samfurin maganin shafawa nan da nan bayan an buɗe shi.

GARGAƊI DA HANKALI
.Ya kamata a rufe kayan aikin kuma a kare shi daga danshi.
Duk samfuran da aka tabbatar za a tabbatar da su ta wasu hanyoyi.
.Duk samfuran za a yi musu la'akari da su a matsayin masu iya gurɓata muhalli.
.KADA a yi amfani da maganin da ya ƙare.
.KADA a musanya sinadaran da ke cikin kayan aiki tare da nau'in da ba a haɗa ba..
.KADA a sake amfani da katunan gwaji da duk wani kayan haɗi da za a iya yarwa.
.Rashin aiki daidai, ko wuce gona da iri ko ƙarancin samfur na iya haifar da karkacewar sakamako.

LKWAIKWAYO
Kamar yadda yake da kowace gwaji da ake amfani da maganin rigakafi na linzamin kwamfuta, akwai yiwuwar tsangwama daga ƙwayoyin rigakafi na ɗan adam (HAMA) a cikin samfurin. Samfuran marasa lafiya waɗanda aka yi musu shirye-shiryen ƙwayoyin rigakafi na monoclonal don ganewar asali ko magani na iya ƙunsar HAMA. Irin waɗannan samfuran na iya haifar da sakamako mara kyau ko mara kyau na ƙarya.
.Wannan sakamakon gwajin an yi shi ne kawai don nazartar asibiti, bai kamata ya zama tushen ganewar asali da magani na asibiti ba, ya kamata a yi la'akari da kula da marasa lafiya da alamunsa sosai,
.tarihin likita, sauran gwaje-gwajen dakin gwaje-gwaje, amsar magani, ilimin cututtuka da sauran bayanai.
.Wannan sinadarin ana amfani da shi ne kawai don gwaje-gwajen jini da na jini. Yana iya ba zai sami sakamako mai kyau ba idan aka yi amfani da shi ga wasu samfura kamar su yau da fitsari da sauransu.
HALAYEN AYYUKA

RNAZARI
1. Hansen JH, da sauransu. Tsoma bakin HAMA ga gwajin rigakafi na Murine Monoclonal Antibody-based [J].J na Clin Immunoassay, 1993,16:294-299.
2. Levinson SS. Yanayin Kwayoyin Haɗaka Masu Yawa da Matsayinsu a Cikin Tsangwama Kan Tsarin Immunoassay[J].J na Clin Immunoassay, 1992,15:108-114.

Maɓallin alamomin da aka yi amfani da su:

	Na'urar Lafiya ta In Vitro Diagnostic
	Mai ƙera
	Ajiye a 2-30℃
	Ranar Karewa
	Kada a sake amfani da shi
	HANKALI
	Duba Umarnin Amfani

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