TE MATUA

Kua pania te kiriuhi o te taputapu whakamātautau ki te Rōpū A me te ārai mate adenovirus i te rohe whakamātautau, me te paturopi IgG rāpeti koati i te rohe whakahaere. Kua pania te papa tapanga ki te tohu fluorescence anti Group A me te adenovirus me te IgG rāpeti i mua. Ina whakamatautauria te tauira pai mō te Rōpū A me te adenovirus, ka honoa te Rōpū A me te adenovirus i roto i te tauira ki te tohu fluorescence anti Rotavirus Rōpū A me te adenovirus, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti. Ina paahitia e te matatini te rohe whakamātautau, ka honoa ki te paturopi paninga anti-Rotavirus Rōpū A me te adenovirus, ka hanga he matatini hou. Mena he kino, kaore he Rotavirus Rōpū A me te ārai mate adenovirus i roto i te tauira, no reira kaore e taea te hanga matatini ārai mate, kaore he raina whero i te rohe kitenga (T). Ahakoa kei roto i te tauira te rotavirus me te adenovirus rōpū A, ka chromatographed te IgG kiore kua tohua ki te rohe whakahaere kounga (C) ka hopukina e te paturopi IgG rāpeti koati. Ka puta he rārangi whero i te rohe whakahaere kounga (C). Ko te rārangi whero te paerewa e puta ana i te rohe whakahaere kounga (C) hei whakatau mena he nui ngā tauira, me te mea he noa te tukanga chromatography. Ka whakamahia hoki hei paerewa whakahaere ā-roto mō ngā matū.

Tukanga Whakamātautau:

1. Whakatuwheratia te taupoki o te ngongo kohi tauira. Kaua e ringihia te wai ki roto i te pounamu.
2. Tangohia te rākau tauira, kua whakaurua ki roto i te tauira paru (māhia rānei he rākau tauira hei tango i te 50mg o te paru), kātahi ka whakahokia te rākau tauira, ka whakapiripiri kia mau, ka rūrū pai, ka whakahokia te mahi kia 3 ngā wā. Tangohia he wāhanga rerekē o te tauira paru i ia wā. Whai muri i te tauira, makahia te rākau tauira ki roto i te ngongo kohikohi paru kei roto te wai whakawera tauira, ka whakapiripiri kia mau te pata. Mena he angiangi ake te paru o te tūroro mate korere, ka taea te whakamahi i te kakau kirihou whakakorekore hei tauira. Mā te whakamahi i te pipette whakakorekore hei tauira, tangohia te tauira paru angiangi mai i te tūroro mate korere, kātahi ka tāpirihia kia 3 ngā pata (tata ki te 100uL) ki te ngongo tauira paru.
3. Rūrūtia te tauira kia pai, ka tangohia te taupoki i te pito o te maturuturunga wai, kātahi ka waiho ki tahaki.
4. Ina rongoatia ki te pāmahana iti, me whakahoki te kete ki te pāmahana rūma i mua i te whakamahinga. Tangohia te kāri whakamātautau mai i te putea pepa konumohe, whakatakotoria ki runga i te tēpu papatahi, ka tohua.
5. Tangohia te taupoki i te ngongo tauira ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 3 ngā pata (tata ki te 100µL) o te tauira kua whakaranua, kia poutū, kia āta haere ki roto i te poka tauira o te kāri me te putanga kua whakaratohia, tīmatahia te wā.
6. Me pānui te hua i roto i ngā meneti 10-15, ā, kāore e whai mana i muri i ngā meneti 15.