UMGAQO

I-membrane yesixhobo sovavanyo igqunywe yi-A kunye ne-adenovirus antigen kwindawo yovavanyo kunye ne-IgG antibody yebhokhwe kwindawo yokulawula. I-Lable pad igqunywe yi-fluorescence ebhalwe yi-anti Group A kunye ne-adenovirus kunye ne-IgG yomvundla kwangaphambili. Xa kuvavanywa isampuli entle ye-A kunye ne-adenovirus, i-A kunye ne-adenovirus kwisampuli zidityaniswe ne-fluorescence ebhalwe yi-anti Rotavirus Group A kunye ne-adenovirus, kwaye zenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, i-complex flow kwicala lephepha elifunxayo. Xa i-complex idlule kwindawo yovavanyo, idibene ne-anti-Rotavirus Group A kunye ne-adenovirus coating antibody, yenza i-complex entsha. Ukuba ayibonakali, akukho Rotavirus Group A kunye ne-adenovirus antigen kwisampuli, ukuze ii-immune complexes zingabikho, akuyi kubakho mgca ubomvu kwindawo yokufumanisa (T). Nokuba i-rotavirus yeqela A kunye ne-adenovirus zikhona na kwisampuli, i-IgG yempuku enelebhile ye-latex ifakwa kwi-chromatograph kwindawo yokulawula umgangatho (C) kwaye ibanjwe yi-antibody ye-IgG ye-mouse anti-mouse. Umgca obomvu uya kuvela kwindawo yokulawula umgangatho (C). Umgca obomvu ngumgangatho obonakala kwindawo yokulawula umgangatho (C) ukuze kugwetywe ukuba kukho iisampulu ezaneleyo na kunye nokuba inkqubo ye-chromatography iqhelekile na. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi lwee-reagents.

Inkqubo yoVavanyo:

1. Vula isigqubuthelo setyhubhu yokuqokelela isampuli. Musa ukuphalaza isisombululo ebhotileni.
2. Khupha intonga yokuvavanya, efakwe kwisampuli yendle (okanye sebenzisa intonga yokuvavanya ukuze ukhethe malunga ne-50mg yendle), uze uyibuyisele intonga yokuvavanya, uyiqinise uze uyishukumise kakuhle, uphinde wenze loo nto izihlandlo ezi-3. Thatha inxalenye eyahlukileyo yesampuli yendle ngalo lonke ixesha. Emva kokuvavanya, faka intonga yokuvavanya kwityhubhu yokuqokelela indle equlethe isampuli yokuxuba, uze uyijije ngokuqinileyo idropha. Ukuba indle yomguli onesifo sorhudo incinci, ingca yeplastiki elahlwayo ingasetyenziselwa ukuthatha isampuli. Usebenzisa isampuli yepipette elahlwayo thatha isampuli yendle elahlwayo kumguli onesifo sorhudo, uze wongeze amathontsi ama-3 (malunga ne-100uL) kwityhubhu yokuvavanya indle.
3. Shukumisa kakuhle isampuli uze ususe isiciko kwincam yedropper uze uyibeke ecaleni.
4. Xa igcinwe kubushushu obuphantsi, ikhithi kufuneka ibuyiselwe kubushushu begumbi ngaphambi kokuba isetyenziselwe. Khupha ikhadi lovavanyo kwibhegi yefoyile, ulibeke etafileni elinganayo uze uliphawule.
5. Susa isiciko kwityhubhu yesampuli uze ulahle amathontsi amabini okuqala esampuli exutyiweyo, yongeza amathontsi amathathu (malunga ne-100uL) isampuli exutyiweyo engenamaqamza ngokuthe nkqo kwaye kancinci kancinci kwiqula lesampuli yekhadi ene-dispette enikiweyo, qala ixesha.
6. Isiphumo kufuneka sifundwe kwimizuzu eli-10-15, kwaye asisebenzi emva kwemizuzu eli-15.