Ikhithi Yokuxilonga ye-Estradiol(ukuhlolwa kwe-fluorescence immunochromatographic)
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuxilonga ye-Estradiol (i-fluorescence immunochromatographic assay) iyi-fluorescence immunochromatographic assay yokuthola inani le-Estradiol (E2) ku-serum noma ku-plasma yomuntu, esetshenziswa kakhulu ukuhlola amazinga e-Estradiol. Iyi-reagent yokuxilongwa eyisizayo. Zonke izibonelo ezinhle kumele ziqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

ISIFINYEZO
I-Estradiol (E2) iyi-hormone ebaluleke kakhulu futhi esebenza kakhulu ku-estrogen. Isisindo sayo sama-molecule singama-272.3 D. Ngokuvamile, kwabesifazane abangakhulelwe, i-E2 ikhishwa kakhulu ngamaseli e-sheath kanye nama-granular kanye namaseli e-luteal ngesikhathi sokuthuthukiswa kwe-follicular. Ngesikhathi sokukhulelwa, i-E2 ikhishwa kakhulu yi-placenta, kuyilapho amadoda ekhiqizwa kakhulu ngama-testicles. Ngemva kokuba i-E2 ingene egazini, u-1% kuya ku-3% awabophi amaprotheni, u-40% ubopha ku-sex hormone binding globulin (SHBG), kanti abanye babopha ku-albumin, baxube kusuka esibindini babe ama-sulfate noma ama-gluconaldehyde esters ancibilikayo emanzini, futhi bakhishwe emchameni. I-E2 iyinkomba ebalulekile ye-hormone yokuhlola ukusebenza kwe-ovarian.

ISIMISO SENQUBO
I-membrane yedivayisi yokuhlola imbozwe nge-conjugate ye-BSA ne-Estradiol esifundeni sokuhlola kanye ne-antibody yembuzi yokulwa nonogwaja IgG esifundeni sokulawula. Iphedi yomaka imbozwe nge-antibody ye-anti-E2 uphawu lwe-fluorescence kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-E2 kusampula ihlangana ne-antibody ye-anti-E2 uphawu lwe-fluorescence, bese yakha ingxube yokuzivikela. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula esifundeni sokuhlola, i-free fluorescent marker izohlanganiswa ne-Estradiol ku-membrane. Ukuhlushwa kwe-Estradiol kuwukuxhumana okungekuhle kwesignali ye-fluorescence, futhi ukuhlushwa kwe-Estradiol kusampula kungatholakala nge-fluorescence immunoassay assay.

IZINTO EZINGENAYO NEZINTO EZINIKEZWE

Izingxenye zephakheji ezingama-25T：
Ikhadi lokuhlola ngalinye lifakwe ifoyili ngesikhwama se-desiccant esingu-25T
Isixazululo .I-25T
Isixazululo se-.B 1
.Iphakheji yokufaka 1

KUDINGEKA IZINTO EZISETSHENZISWAYO KODWA AZINIKEZWA
Isitsha sokuqoqa isampula, isibali sikhathi

IQOQO LEZIBONELO NOKUGCINA
1. Amasampula ahlolwe angaba yi-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2. Ngokwezindlela ezijwayelekile zokuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku eziyi-7 kanye nokugcinwa ngaphansi kuka-15°C izinyanga eziyi-6.
.Zonke izibonelo zigwema imijikelezo yokuqandisa nokuncibilikisa.

INQUBO YOKUHLOLA
Inqubo yokuhlola yale thuluzi bheka incwadi ye-immunoanalyzer. Inqubo yokuhlola i-reagent imi kanje
1. Beka eceleni wonke ama-reagent namasampula ukuze izinga lokushisa legumbi lifinyelele ezingeni lokushisa legumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3.Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
3. Khipha ikhadi lokuhlola esikhwameni se-foil.
4.Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
5. Faka isampula ye-serum noma i-plasma engu-30μL esixazululweni se-A, bese uxuba kahle.
6. Faka isisombululo se-20μL B engxubeni engenhla, bese uxuba kahle.
Shiya ingxube ukuze20imizuzu.
Faka ingxube engu-80μL ukuze uhlole umthombo wekhadi.
Chofoza inkinobho ethi “isivivinyo esijwayelekile”, ngemva kwemizuzu eyi-10, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

IMIPHUMELA YOKUHLOLA NOKUHLUKANISWA

.Idatha engenhla iyisikhathi sokubhekisela esimiselwe idatha yokuthola yale khithi, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule isikhathi sokubhekisela sokubaluleka komtholampilo okufanelekile kwabantu kulesi sifunda.
.Izinga le-Estradiol liphakeme kunezinga elibhekiselwe kulo, futhi izinguquko zomzimba noma impendulo yokucindezeleka kufanele ikhishwe. Ngempela okungavamile, kufanele kuhlanganiswe ukuxilongwa kwezimpawu zomtholampilo.
.Imiphumela yale ndlela isebenza kuphela kububanzi bokubhekisela obusungulwe yile ndlela, futhi imiphumela ayifani ngqo nezinye izindlela.
Ezinye izici zingadala amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza kanye nezinye izici zesampula.

ISITOKELO NOKUZINZIMA
1. Ikhithi ihlala izinyanga ezingu-18 kusukela ngosuku lokwenziwa kwayo. Gcina amakhithi angasetshenziswanga ku-2-30°C. UNGAWAQANDISI. Ungayisebenzisi ngemva kosuku lokuphelelwa yisikhathi.
2. Ungavuli isikhwama esivaliwe uze ulungele ukwenza isivivinyo, futhi isivivinyo sokusetshenziswa kanye sinconywa ukuthi sisetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa 2-35℃, umswakama 40-90%) zingakapheli imizuzu engama-60 ngokushesha okukhulu.
3. Isampula yokuxuba isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQAPHELO ZOKWENZA
.Ikhithi kufanele ivalwe futhi ivikelwe emswakama.
Zonke izibonelo ezinhle kufanele ziqinisekiswe ngezinye izindlela.
Zonke izibonelo kufanele ziphathwe njengezingcolisi ezingaba khona.
UNGASEBENZISI i-reagent ephelelwe yisikhathi.
UNGASHINTSHI ama-reagent phakathi kwamakhithi anenombolo ehlukene yezindawo..
UNGASEBENZISI kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Ukusebenza kabi, isampula eningi kakhulu noma encane kungaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukuhlolwa okusebenzisa ama-antibodies egundane, kungenzeka ukuthi kukhona ukuphazamiseka kwama-antibodies e-anti-mouse (HAMA) esampula. Amasampula avela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal ukuze kutholakale isifo noma ukwelashwa angase abe ne-HAMA. Amasampula anjalo angabangela imiphumela emibi noma emibi engamanga.
.Lo mphumela wokuhlolwa ungowokubhekisela emtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kwezokwelapha kuphela, ukuphathwa kwezokwelapha kweziguli kufanele kucatshangelwe ngokuphelele kuhlanganiswe nezimpawu zako,
.umlando wezokwelapha, okunye ukuhlolwa kwelabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi.
.Le reagent isetshenziswa kuphela ekuhlolweni kwe-serum kanye ne-plasma. Ingase ingatholi umphumela onembile uma isetshenziswa kwamanye amasampula afana namathe nomchamo njll.
IZIMALI ZOKUSEBENZA

RIZINKOMBA
1.Hansen JH, nabanye.Ukuphazamiseka kwe-HAMA nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Uhlobo Lwama-Antibodies Angama-Heterophilic kanye Nendima Ekuphazamisekeni Kwe-Immunoassay[J].J we-Clin Immunoassay,1992,15:108-114.

Isihluthulelo sezimpawu ezisetshenzisiwe:

	Idivayisi Yezokwelapha Yokuxilonga E-In Vitro
	Umkhiqizi
	Gcina ku-2-30℃
	Usuku lokuphelelwa isikhathi
	Ungasebenzisi Kabusha
	ISEXWAYISO
	Bheka Imiyalelo Yokusebenzisa

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