ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-Group A kanye ne-adenovirus antigen endaweni yokuhlola kanye ne-goat anti-rabbit IgG antibody endaweni yokulawula. I-lable pad imbozwe nge-fluorescence ebhalwe nge-anti-Group A kanye ne-adenovirus kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula enhle ye-Group A kanye ne-adenovirus, i-Group A kanye ne-adenovirus kusampula kuhlangana ne-fluorescence ebhalwe nge-anti-Rotavirus Group A kanye ne-adenovirus, bese kwakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza kwe-complex kuya ohlangothini lwephepha elimuncayo. Lapho i-complex idlula esifundeni sokuhlola, ihlanganiswe ne-anti-Rotavirus Group A kanye ne-anti-adenovirus coating antibody, yakha i-complex entsha. Uma i-negative, ayikho i-Rotavirus Group A kanye ne-adenovirus antigen kusampula, ukuze ama-immune complexes angakhiwa, ngeke kube khona umugqa obomvu endaweni yokuthola (T). Kungakhathaliseki ukuthi i-rotavirus yeqembu A kanye ne-adenovirus zikhona yini kusampula, i-IgG yegundane enelebula le-latex ifakwa i-chromatograph endaweni yokulawula ikhwalithi (C) futhi ibanjwe yi-antibody ye-IgG yegundane elwa negundane. Umugqa obomvu uzovela endaweni yokulawula ikhwalithi (C). Umugqa obomvu uyindinganiso ebonakala endaweni yokulawula ikhwalithi (C) ukuze kunqunywe ukuthi kukhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Isetshenziswa futhi njengendinganiso yokulawula yangaphakathi yama-reagents.

Inqubo Yokuhlola:

1. Vula isivalo sethubhu lokuqoqa isampula. Ungalichitheli ikhambi ebhodleleni.
2. Khipha induku yokuqoqa isampula, efakwe kusampula yendle (noma sebenzisa induku yokuqoqa isampula ukuze ukhethe cishe u-50mg wendle), bese ubuyisela induku yokuqoqa isampula, uyiqinise bese uyinyakazisa kahle, uphinde wenze lokhu kathathu. Thatha ingxenye ehlukile yesampula yendle isikhathi ngasinye. Ngemva kokuthatha isampula, faka induku yokuqoqa indle equkethe isampula yokuxuba, bese uyiqinisa nge-dropper. Uma indle yesiguli esinesifo sohudo incane, utshani lwepulasitiki olulahlwayo lungasetshenziswa ukuthatha isampula. Usebenzisa isampula yepayipi elilahlwayo thatha isampula yendle encane esigulini esinesifo sohudo, bese ufaka amaconsi amathathu (cishe u-100uL) epayipini lokuqoqa indle.
3. Shukumisa kahle isampula bese ususa isivalo esiqongweni se-dropper bese usibeka eceleni.
4. Uma igcinwe ezingeni lokushisa eliphansi, ikhithi kufanele ibuyiselwe ezingeni lokushisa legumbi ngaphambi kokusebenzisa. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elilinganayo bese ulimaka.
5. Susa isivalo epayipini lesampula bese ulahla amaconsi amabili okuqala esampula exutshwe, engeza amaconsi amathathu (cishe ama-uL ayi-100) isampula exutshwe ngamabhamuza iqonde futhi kancane kancane emthonjeni wesampula wekhadi nge-dispette enikeziwe, qala isikhathi.
6. Umphumela kufanele ufundwe zingakapheli imizuzu eyi-10-15, futhi awusebenzi ngemva kwemizuzu eyi-15.