Agakoresho ko gusuzuma indwara ya Estradiol(isuzuma rya fluorescence immunochromatographic assay)
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE
Ikizamini cyo gusuzuma Estradiol (ikizamini cya fluorescence immunochromatographic assay) ni ikizamini cya fluorescence immunochromatographic assay cyo gupima Estradiol (E2) mu maraso y'umuntu cyangwa mu maraso, gikoreshwa cyane cyane mu gusuzuma urugero rwa Estradiol. Ni reagent y'inyongera yo gusuzuma. Icyitegererezo cyose cyagaragaye ko gifite ubwandu kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE
Estradiol (E2) ni yo misemburo y'ingenzi kandi ikora cyane muri estrogen. Uburemere bwayo bwa molekile ni 272.3 D. Muri rusange, ku bagore badatwite, E2 isohoka ahanini n'uturemangingo tw'igishishwa n'uturemangingo tw'uruti n'uturemangingo tw'uruti rw'umura mu gihe cy'imikurire ya follicular. Mu gihe cyo gutwita, E2 isohoka ahanini n'ingobyi, mu gihe abagabo bakorwa cyane n'udusabo tw'intanga. Iyo E2 imaze kwinjira mu maraso, 1% kugeza kuri 3% ntibifatanya na poroteyine, 40% bifatanya na globulin ihuza imisemburo y'igitsina (SHBG), naho izindi zifatanya na albumin, zigahinduka sulfates cyangwa gluconaldehyde esters, hanyuma zigasohoka mu nkari. E2 ni ikimenyetso cy'ingenzi cy'imisemburo mu gusuzuma imikorere y'intanga.

IHAME RY'UBURYO BWAYO
Agace k'igikoresho cyo gupima gatwikiriwe n'agace ka BSA na Estradiol ku gice cy'igeragezwa n'agace ka anti-inkwavu ka IgG ku gice cyo kugenzura. Agace karatwikiriwe n'agace ka anti-inkwavu ka E2 kari ku kimenyetso cy'urumuri mbere y'igihe. Mu gihe cyo gupima icyitegererezo, E2 mu gipimo ihuzwa n'agace ka anti-inkwavu ka E2 kari ku kimenyetso cy'urumuri, maze igakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'igikorwa cya immunochromatography, urujya n'uruza rw'urumuri mu cyerekezo cy'impapuro zifata, iyo agace kanyuze mu gice cy'igeragezwa, ikimenyetso cy'urumuri cyigenga kizahuzwa na Estradiol ku gipimo. Ingano ya Estradiol ni isano iri hagati y'ikimenyetso cy'urumuri, kandi ingano ya Estradiol mu gipimo ishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw'umubiri.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:
Ikarita yo gupima ku giti cyayo ipfunyitse mu gapfunyika gafite agakoresho ko gusukura 25T
.Igisubizo 25T
Igisubizo cya .B 1
.Ipaki ishyirwamo 1

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
1. Ingero zapimwe zishobora kuba serum, heparin anticoagulant plasma cyangwa EDTA anticoagulant plasma.
2. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6.
Ingero zose zirinda gukonjesha no gushonga.

UBURYO BWO GUKORA IKIGANIRO
Uburyo bwo gupima igikoresho reba igitabo cy'amabwiriza agenga ikoreshwa ry'imyunyungugu. Uburyo bwo gupima reagent ni ubu bukurikira
1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
3. Kura ikarita yo gupima mu gafuka k'ifishi.
4. Shyira ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
5. Shyiramo serum cyangwa plasma ya 30μL mu gisubizo cya A, hanyuma uvange neza.
6. Shyiramo umuti wa 20μL B mu ruvange rwavuzwe haruguru, hanyuma uvange neza.
Siga uruvange kugira ngo20iminota.
Shyiramo uruvange rwa 80μL kugira ngo ufate urugero rw'aho ikarita iherereye.
Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 10, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO

.Amakuru yavuzwe haruguru ni igihe cyo gusuzuma amakuru y’iyi kit, kandi birasabwa ko buri laboratwari igomba gushyiraho igihe cyo gusuzuma amakuru ajyanye n’akamaro k’ubuvuzi ku baturage bo muri aka gace.
.Ingano ya Estradiol iri hejuru y’urugero rw’ifatizo, kandi impinduka z’umubiri cyangwa igisubizo cy’imihangayiko ntizigomba kurebwa. Mu by’ukuri, bigomba kuba bihuriweho no gusuzuma ibimenyetso by’uburwayi.
Ibisubizo by'ubu buryo bireba gusa urwego rw'ifatizo rwashyizweho n'ubu buryo, kandi ibisubizo ntibigereranywa neza n'ubundi buryo.
Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE
1. Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ikozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
2. Ntugafungure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
3. Icyitegererezo cy'umuti uvangwa n'amazi gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
.Isanduku igomba kuba ifunze kandi irinzwe ubushuhe.
Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
Ibipimo byose bigomba gufatwa nk'ibintu bishobora kwanduza.
NTUKORESHE reagent yarengeje igihe.
NTUGAHINDURE ibyuma bitanga ubuziranenge hagati y'ibikoresho bifite ubwoko butandukanye..
NTUGAKORESHE gukoresha amakarita yo gupima n'ibindi bikoresho byo gukoresha mu gihe cyo kuyakoresha.
.Gukoresha nabi, icyitegererezo gikabije cyangwa gito bishobora gutuma ibisubizo bihinduka.

LKWIGANA
Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabwo,
.amateka y'ubuzima, ubundi buryo bwo gusuzuma muri laboratwari, uburyo bwo kuvurwa, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima amaraso mu maraso no mu maraso. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.
IBIRANGA IMIKORERE

RIBIREBANA
1.Hansen JH, et al.HAMA Interference hamwe n'ibizamini by'imyuka bishingiye ku murine monoclonal antibody [J].J bya Clin Immunoassay, 1993,16:294-299.
2.Levinson SS. Imiterere y'uturemangingo tw'umubiri duto duto n'uruhare mu gutera ikibazo cy'ubudahangarwa bw'umubiri [J].J ya Clin Immunoassay, 1992,15:108-114.

Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha

Xiamen Wiz Biotech CO.,LTD
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