Ikhithi Yokuxilonga I-Insulin Yokuphathwa Kwesifo Sikashukela
Ikhithi Yokuxilonga ye-Insulin
Indlela Yokusebenza: Ukuhlolwa Kwe-Fluorescence Immunochromatographic
Ulwazi lokukhiqiza
| Inombolo Yemodeli | I-INS | Ukupakisha | Izivivinyo ezingu-25/ikhithi, amakhithi angu-30/i-CTN |
| Igama | Ikhithi Yokuxilonga ye-Insulin | Ukuhlukaniswa kwezinsimbi | Iklasi II |
| Izici | Ukuzwela okuphezulu, Ukusebenza okulula | Isitifiketi | I-CE/ ISO13485 |
| Ukunemba | > 99% | Isikhathi sokuphelelwa yisikhathi | Iminyaka Emibili |
| Indlela Yokwenza | Ukuhlolwa kwe-Fluorescence Immunochromatographic | Isevisi ye-OEM/ODM | Kuyatholakala |
Ukuphakama
Isikhathi sokuhlola: 10-15mins
Isitoreji: 2-30℃/36-86℉
Indlela Yokusebenza: Ukuhlolwa Kwe-Fluorescence Immunochromatographic
UKUSETSHENZISWA OKUHLOSIWE
Leli khithi lifanelekela ukunqunywa kwesilinganiso samazinga e-insulin (INS) ku-serum/plasma/amasampula egazi lomuntu wonke ukuze kuhlolwe ukusebenza kwe-pancreatic-islet β-cell. Leli khithi linikeza imiphumela yokuhlola i-insulin (INS) kuphela, futhi umphumela otholiwe kufanele uhlaziywe uhlanganiswe nolunye ulwazi lwezokwelapha. Umphumela kufanele uhlaziywe uhlanganiswe nolunye ulwazi lwezokwelapha.
Isici:
• Ukuzwela okuphezulu
• ukufundwa kwemiphumela ngemizuzu eyi-15
• Ukusebenza okulula
• Ukunemba Okuphezulu
Inqubo yokuhlola
| 1 | Ngaphambi kokusebenzisa i-reagent, funda ngokucophelela iphakheji bese ujwayelana nezinqubo zokusebenza. |
| 2 | Khetha imodi yokuhlola ejwayelekile ye-WIZ-A101 portable immune analyzer |
| 3 | Vula iphakethe lesikhwama se-aluminium foil eline-reagent bese ukhipha idivayisi yokuhlola. |
| 4 | Faka idivayisi yokuhlola ngokuvundlile endaweni yokuhlaziya amasosha omzimba. |
| 5 | Ekhasini lasekhaya le-interface yokusebenza ye-immune analyzer, chofoza okuthi “Standard” ukuze ufake i-interface yokuhlola. |
| 6 | Chofoza okuthi “QC Scan” ukuze uskene ikhodi ye-QR ngaphakathi kwekhithi; amapharamitha ahlobene nekhithi yokufaka kuthuluzi bese ukhetha uhlobo lwesampula. Qaphela: Inombolo ngayinye yebhetshi yekhithi kufanele iskeniwe kanye. Uma inombolo yebhetshi isiskeniwe, yeqa lesi sinyathelo. |
| 7 | Hlola ukuvumelana “kwegama lomkhiqizo”, “inombolo yeqoqo” njll. esibonakalayo sokuhlola ngolwazi oluselebula lekhithi. |
| 8 | Khipha isampula yokuxuba uma ulwazi luhlala lukhona, engeza isampula yegazi eliyi-10μL/i-plasma/igazi lonke, bese ulixuba kahle; |
| 9 | Faka isixazululo esixutshwe kahle esingu-80µL esishiwo ngenhla emthonjeni wedivayisi yokuhlola; |
| 10 | Ngemva kokwengeza okuphelele kwesampula, chofoza okuthi “Isikhathi” bese isikhathi sokuhlola esisele sizoboniswa ngokuzenzakalelayo ku-interface. |
| 11 | I-immune analyzer izoqedela ngokuzenzakalelayo ukuhlolwa nokuhlaziywa lapho isikhathi sokuhlolwa sesifikile. |
| 12 | Ngemva kokuthi ukuhlolwa yi-immune analyzer sekuqediwe, umphumela wokuhlolwa uzoboniswa ku-interface yokuhlola noma ungabukwa "ngomlando" ekhasini lasekhaya le-interface yokusebenza. |
Qaphela: isampula ngayinye kufanele ifakwe ngepayipi elihlanzekile elilahlwayo ukuze kugwenywe ukungcola okuxubile.
Ukusebenza Kwezokwelapha
Ukusebenza kokuhlolwa kwezokwelapha kwalo mkhiqizo kuhlolwe ngokuqoqa amasampula ezokwelapha angu-173. Imiphumela yokuhlolwa iqhathaniswe kusetshenziswa amakhithi ahambisanayo endlela ye-electrochemiluminescence ethengiswayo njengezinto zokubhekisela, futhi ukuqhathanisa kwawo kuhlolwe ngokuhlelwa kabusha okuqondile, kanti ama-coefficients okuxhumana kwezivivinyo ezimbili ayengu-y = 0.987x+4.401 kanye no-R = 0.9874, ngokulandelana.
