Ngwa Nchọpụta Ọrịa maka Antigen na Helicobacter Pylori(Nnwale Immunochromatographic nke Fluorescence)
Maka ojiji nchọpụta n'ime vitro naanị
Biko gụọ ihe e tinyere na ngwugwu a nke ọma tupu e jiri ya, ma gbasoo ntuziaka ndị a nke ọma. A gaghị ekwe nkwa na nsonaazụ nnwale ga-adabere na ntụkwasị obi ma ọ bụrụ na e nwere ihe ọ bụla na-adịghị mma na ntuziaka dị na ngwugwu a.

Ebumnobi Ojiji
Ngwa Nchọpụta maka Antigen ruo Helicobacter Pylori (Fluorescence Immunochromatographic Assay) dabara adaba maka nchọpụta ọnụọgụ nke nje HP antigen mmadụ site na nnwale fluorescence immunochromatographic, nke nwere uru nchọpụta ngwa ngwa maka ọrịa afọ. A ga-enwerịrị usoro ndị ọzọ iji kwado ihe nlele niile dị mma. Emebere ule a maka ojiji ndị ọkachamara ahụike naanị.

Nchịkọta
Ọrịa Helicobacter pylori dị n'ime afọ nwere njikọ chiri anya na gastritis na-adịghị ala ala, ọnya afọ, adenocarcinoma afọ, lymphoma nke metụtara mucosa afọ, ọnụego ọrịa Hp ylori nke ihe dị ka 90% na gastritis, ọnya afọ, ọnya afọ na ndị nwere kansa afọ. Òtù ahụike ụwa achọpụtala h. pylori dị ka ụdị mbụ nke ihe na-akpata ọrịa kansa ma bụrụkwa ihe nwere ike ibute ọrịa kansa afọ. Nchọpụta H. pylori bara ezigbo uru na nchọpụta nke ọrịa H. pylori. Nnwale ahụ dabere na teknụzụ nyocha fluorescence immunochromatographic, nke nwere ike inye nsonaazụ n'ime nkeji iri na ise.

ỤKPỤRỤ NKE ỤZỌ A

Eriri ahụ nwere ihe mgbochi mkpuchi HP na mpaghara nnwale, nke a na-ejikọ na membrane chromatography tupu oge eruo. A na-ekpuchi paadị label site na fluorescence nke a na-akpọ anti-HP antibody tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, enwere ike ịgwakọta HP dị na ihe nlele ahụ na fluorescence nke a na-akpọ anti-HP antibody, wee mepụta ngwakọta mgbochi. Ebe ọ bụ na a na-ekwe ka ngwakọta ahụ kwaga n'akụkụ mpempe ule ahụ, ihe mgbochi mkpuchi HP na-ejide ihe mgbagwoju anya HP conjugate na membrane ahụ ma mepụta mgbagwoju anya. Ike fluorescence ahụ na-ejikọta nke ọma na ọdịnaya HP. Enwere ike ịchọpụta HP dị na ihe nlele ahụ site na ihe nyocha fluorescence immunoassay.

Ihe ndị na-eme ka ihe dị nro na ihe ndị e ji mee ihe dị iche iche

Ihe mejupụtara ngwugwu 25T:
Kaadị nnwale n'otu n'otu nke ejiri foil kpuchie akpa ya na ihe nhicha mmiri 25T
Ihe ndị na-agbaze ihe nlele 25T
Ntinye ngwugwu 1

A CHỌRỌ IHE E JI MEE YA MA E NWEGHỊ YA
Ihe nlele nchịkọta akpa, ihe ngụ oge

Nchịkọta na Nchekwa ihe nlele
1. Jiri ihe dị ọcha a na-atụfu atụfu chịkọta ihe nlele nsị ọhụrụ, wee nwalee ya ozugbo. Ọ bụrụ na ị gaghị anwale ya ozugbo, biko debe ya na okpomọkụ 2-8°C ruo ụbọchị atọ ma ọ bụ karịa -15°C ruo ọnwa isii.
2. Wepụ ihe nlele ahụ, tinye ya n'ime ihe nlele nsị ahụ, megharịa ihe ahụ ugboro atọ, were akụkụ dị iche iche nke ihe nlele nsị ahụ oge ọ bụla, wee tinye ihe nlele ahụ azụ, kpụchie ya nke ọma ma maa jijiji nke ọma, Ma ọ bụ jiri ihe nlele ahụ ahọrọ ihe nlele nsị ahụ ihe dị ka 50mg, tinye ya na tube ihe nlele nsị nke nwere ihe nlele ahụ, wee kpụchie ya nke ọma.
3. Jiri ihe nlele pipette a na-atụfu atụfu, were ihe nlele nsị n'aka onye ọrịa afọ ọsịsa, wee tinye ntapụ atọ (ihe dị ka 100µL) n'ime tube ihe nlele nsị wee maa jijiji nke ọma.

Ihe ndị e dere:
1. Zere usoro ịgbaze na ịgbaze.
2. Mee ka ihe nlele ahụ gbazee ruo okpomọkụ ụlọ tupu e jiri ya.

Usoro Nnwale
Biko gụọ akwụkwọ ntuziaka ọrụ ngwa ahụ na ihe e tinyere na ngwugwu tupu ị nwaa ya.
1. Debe ihe niile e ji eme ihe na ihe nlele n'akụkụ okpomọkụ ụlọ.
2. Mepee Portable Immune Analyzer (WIZ-A101), tinye paswọọdụ akaụntụ dịka usoro ọrụ nke ngwa ahụ si dị, wee tinye interface nchọpụta.
3. Lelee koodu dentification iji kwado ihe nnwale ahụ.
4. Wepụ kaadị ule ahụ n'ime akpa foil ahụ.
5. Tinye kaadị ule ahụ n'ime oghere kaadị ahụ, lelee koodu QR ahụ, wee chọpụta ihe nnwale ahụ.
6. Wepụ mkpuchi ahụ n'ime ọkpọkọ ihe nlele ahụ wee tụfuo ntapụ abụọ mbụ nke ihe nlele ahụ a gwakọtara agwakọta, tinye ntapụ atọ (ihe dị ka 100uL) nke ihe nlele ahụ a gwakọtara agwakọta kwụ ọtọ ma jiri nwayọ banye n'ime olulu ihe nlele nke kaadị ahụ yana ihe nlele enyere.
7. Pịa bọtịnụ "ule ọkọlọtọ", mgbe nkeji iri na ise gachara, ngwaọrụ ahụ ga-achọpụta kaadị nnwale ahụ na akpaghị aka, ọ nwere ike ịgụ nsonaazụ sitere na ihuenyo ngosipụta nke ngwaọrụ ahụ, ma dekọọ/bipụta nsonaazụ nnwale ahụ.
8. Lee ntuziaka nke Portable Immune Analyzer (WIZ-A101).

Uru A Na-atụ Anya Ya
HP-Ag <10
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya nke na-anọchite anya ọnụọgụ ndị ọrịa ya.

Nsonaazụ na Nkọwa nke Ule
1. HP-Ag dị na ihe nlele ahụ karịrị 10, ọ kwesịkwara iwepụ mgbanwe nke ọnọdụ ahụ. Nsonaazụ ya bụ ihe na-adịghị mma ma a ga-achọpụta na ha nwere mgbaàmà ahụike.
2. Nsonaazụ nke usoro a metụtara naanị oke ntụaka e guzobere n'ụzọ a, ọ dịghịkwa ntụnyere kpọmkwem na ụzọ ndị ọzọ.
3. Ihe ndị ọzọ nwekwara ike ịkpata mmejọ na nsonaazụ nchọpụta, gụnyere ihe ndị metụtara teknụzụ, mmejọ arụmọrụ na ihe atụ ndị ọzọ.

Nchekwa na nkwụsi ike
1. Ngwa a ga-adịru ọnwa iri na asatọ site na ụbọchị e mepụtara ya. Debe ngwa ndị a na-ejighị na okpomọkụ 2-30°C. EKPERELA FRỊA. Ejila ya karịa ụbọchị njedebe.
2. Emeghela akpa ahụ e mechiri emechi ruo mgbe ị dị njikere ime nnwale, a na-atụkwa aro ka e jiri ule ahụ otu ugboro mee ihe n'okpuru ebe achọrọ (okpomọkụ 2-35℃, iru mmiri 40-90%) n'ime nkeji iri isii ozugbo enwere ike.
3. A na-eji ihe nlele sample ozugbo emepechara ya.

ỊDỌ AKA NA NTỊ NA ỊKPACHICHE
.A ga-emechi ngwa ahụ ma chebe ya pụọ ​​na mmiri mmiri.
. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma.
A ga-ewere ihe nlele niile dị ka ihe nwere ike imetọ ihe.
.EJILA ihe na-eme ka ihe ghara ịdị irè.
.EJILA ihe ndị na-agbanwe ihe n'etiti ngwa na ọtụtụ ihe dị iche iche.
.EJILA kaadị ule na ihe ndị ọzọ a na-atụfu atụfu eme ihe ọzọ.
.Ịrụ ọrụ na-ezighi ezi, oke ma ọ bụ obere ihe nlele nwere ike ibute mgbanwe nsonaazụ.

LNṄỤRỤ
Dịka ọ dị na nnwale ọ bụla ejiri ọgwụ mgbochi òké mee, o nwere ike inwe nsogbu site na ọgwụ mgbochi òké mmadụ (HAMA) na ihe nlele ahụ. Ihe nlele sitere n'aka ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchọpụta ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ihe nlele ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ nsonaazụ na-adịghị mma.
.Nsonaazụ ule a bụ naanị maka ntụaka ahụike, ekwesịghị ịbụ naanị ntọala maka nchọpụta na ọgwụgwọ ahụike, njikwa ahụike nke ndị ọrịa kwesịrị ịtụle nke ọma yana mgbaàmà ya, akụkọ ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ.
.A na-eji ihe a eme ihe naanị maka ule nsị. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dịka ọnụ mmiri na mmamịrị na wdg.

ÀGWÀ ÀGWÀ Arụmọrụ

RNtụaka

1.Shao,JL&F.Wu.Ọganihu ndị a na-eme n'oge na-adịbeghị anya n'ụzọ nchọpụta nke Helicobacter pylori[J].Journal of Gastroenterology and Hepatology,2012,21(8):691-694
2. Hansen JH, na ndị ọzọ. Mgbochi HAMA na ọgwụ mgbochi ọrịa nke dabere na mmadụ site na Murine Monoclonal [J].J nke Clin Immunoassay, 1993,16:294-299.
3. Levinson SS. Ọdịdị nke Ọgwụ Mgbochi Heterophilic na Ọrụ dị na Mgbochi Immunoassay[J].J nke Clin Immunoassay, 1992,15:108-114.

Isi ihe nnọchianya ejiri mee ihe:

	Ngwaọrụ Ahụike Nchọpụta In Vitro
	Onye nrụpụta
	Chekwaa na okpomọkụ 2-30℃
	Ụbọchị mmebi
	Ejila ya ọzọ
	ỊDỌ AKA NA NTỊ
	Lelee Ntuziaka Maka Ojiji

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