Ikhithi yokuxilonga ye-Antigen ukuya kwi-Helicobacter Pylori(Uvavanyo lwe-Fluorescence Immunochromatographic)
Ukusetyenziswa kokuxilongwa kwi-vitro kuphela
Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO
Ikhithi yokuxilonga ye-Antigen ukuya kwi-Helicobacter Pylori (Uvavanyo lwe-Fluorescence Immunochromatographic) ifanelekile ekuchongeni ubungakanani be-HP antigen yelindle lomntu ngovavanyo lwe-fluorescence immunochromatographic, olunexabiso elibalulekileyo lokuxilonga usulelo lwesisu. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zonyango kuphela.

ISISHWANKATHELO
Usulelo lwesisu i-helicobacter pylori lunxulumene kakhulu ne-gastritis engapheliyo, isilonda sesisu, i-adenocarcinoma yesisu, i-lymphoma enxulumene ne-mucosa yesisu, izinga losulelo lwe-Hp ylori elimalunga ne-90% kwizigulane ezine-gastritis, isilonda sesisu, isilonda se-duodenal kunye nomhlaza wesisu. Umbutho wezempilo wehlabathi uchonge i-h. pylori njengohlobo lokuqala lwento ebangela umhlaza kwaye ngokucacileyo luyimbangela yomngcipheko womhlaza wesisu. Ukufunyanwa kwe-H. pylori kubaluleke kakhulu ekuxilongweni kosulelo lwe-h. pylori. Uvavanyo olusekelwe kwi-fluorescence immunochromatographic assay analysis technics, enokunika iziphumo kwimizuzu eli-15.

UMGAQO WENKQUBO

I-strip ine-anti-HP coating antibody kwindawo yovavanyo, ebotshelelwe kwi-membrane chromatography kwangaphambili. I-Lable pad igqunywe yi-fluorescence ebhalwe yi-anti-HP antibody kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-HP kwisampuli inokuxutywa ne-fluorescence ebhalwe yi-anti-HP antibody, kwaye yenze umxube we-immune. Njengoko umxube uvunyelwe ukuba uhambe ecaleni kwe-test strip, i-HP conjugate complex ibanjwa yi-anti-HP coating antibody kwi-membrane kwaye yenze i-complex. Ubunzulu be-fluorescence buhambelana kakuhle nomxholo we-HP. I-HP kwisampuli inokubonwa nge-fluorescence immunoassay analyzer.

IZINTO EZINIKEZELWAYO NEZIXHOBO

Izixhobo zephakheji ze-25T：
Ikhadi lovavanyo lifakwe ifoyile nganye nganye ene-desiccant engama-25T
Iisampulu zokuxuba i-25T
Iphakheji efakiweyo 1

IZIXHOBO EZIFUNEKAYO KODWA AZINIKELWANGA
Isikhongozeli sokuqokelela isampuli, isibali-xesha

IMFUNDO YOKUQOKELELA NOKUGCINA IMIZEKELO
1. Sebenzisa isitya esicocekileyo esilahlwayo ukuze uqokelele isampuli yendle entsha, kwaye uvavanywe ngoko nangoko. Ukuba awukwazi ukuvavanywa ngoko nangoko, nceda ugcine kumaqondo obushushu angama-2-8°C kangangeentsuku ezi-3 okanye ngaphantsi kwe-15°C kangangeenyanga ezi-6.
2. Khupha intonga yokuvavanya, uyifake kwisampuli yendle, uphinde isenzo izihlandlo ezi-3, thatha iindawo ezahlukeneyo zesampuli yendle ngalo lonke ixesha, uze uyibuyisele intonga yokuvavanya, uyiqinise uze uyishukumise kakuhle, Okanye usebenzisa intonga yokuvavanya ukhethe malunga ne-50mg yesampuli yendle, uze ufake ityhubhu yesampuli yendle equlethe isampuli yokuxutywa, uze uyijije kakuhle.
3.Sebenzisa isampuli yepayipi elahlwayo, thabatha isampuli yendle yomntu onesifo sorhudo, uze wongeze amathontsi amathathu (malunga ne-100µL) kwityhubhu yokuvavanya ilindle uze ushukumise kakuhle.

Amanqaku:
1. Ziphephe ii-freeze-thicken cycles.
2. Nyibilikisa iisampulu zibe kubushushu begumbi ngaphambi kokuba uzisebenzise.

INKQUBO YOVAVANYO
Nceda ufunde incwadi yemiyalelo yokusebenza kwesixhobo kunye nesihlomelo sephakheji ngaphambi kokuvavanya.
1. Beka ecaleni zonke ii-reagents kunye neesampuli ukuya kubushushu begumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokugqitha le-akhawunti ngokwendlela yokusebenza kwesixhobo, uze ufake i-interface yokubona.
3.Skena ikhowudi yokuchonga ukuze uqinisekise into yovavanyo.
4.Khupha ikhadi lovavanyo kwingxowa yefoyile.
5.Faka ikhadi lovavanyo kwindawo yokubeka ikhadi, skena ikhowudi ye-QR, uze umisele into yovavanyo.
6. Susa isiciko kwityhubhu yesampulu uze ulahle amathontsi amabini okuqala esampuli exutyiweyo, yongeza amathontsi amathathu (malunga ne-100uL) isampuli exutyiweyo engaxutyiweyo ngokuthe nkqo uze kancinci ungene emthonjeni wesampulu yekhadi ene-dispette enikiweyo.
7.Cofa iqhosha elithi “uvavanyo oluqhelekileyo”, emva kwemizuzu eli-15, isixhobo siza kuzibona ngokuzenzekelayo ikhadi lovavanyo, singafunda iziphumo kwisikrini sokubonisa isixhobo, size sirekhode/siprinte iziphumo zovavanyo.
8. Jonga imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

IINQOBO EZILINDELEKILEYO
I-HP-Ag<10
Kucetyiswa ukuba ilabhoratri nganye imisele uluhlu lwayo oluqhelekileyo olumele inani lezigulana zayo.

IZIPHUMO ZOVAVANYO NOKUTOLIKWA
1. I-HP-Ag kwisampulu ingaphezulu kwe-10, kwaye kufuneka ithintele utshintsho kwimeko yomzimba. Iziphumo aziqhelekanga ngokwenene kwaye kufuneka zifunyaniswe zineempawu zeklinikhi.
2. Iziphumo zale ndlela zisebenza kuphela kuluhlu lwereferensi olusekwe kule ndlela, kwaye akukho kuthelekiswa ngokuthe ngqo nezinye iindlela.
3.Ezinye izinto zinokubangela iimpazamo kwiziphumo zokufumanisa, kuquka izizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOKUZINZISA
1. Le khithi ihlala iinyanga ezili-18 ukususela kumhla wokwenziwa kwayo. Gcina ezi khithi zingasetyenziswanga kwi-2-30°C. SUKUZIQANDA. Musa ukuzisebenzisa emva komhla wokuphelelwa.
2. Musa ukuvula isikhwama esivaliweyo ude ulungele ukwenza uvavanyo, kwaye uvavanyo olusetyenziswa kanye lucetyiswa ukuba lusetyenziswe phantsi kweemeko ezifunekayo (ubushushu obuyi-2-35℃, umswakama oyi-40-90%) kwimizuzu engama-60 ngokukhawuleza okukhulu.
3. Isampulu yomxube isetyenziswa kwangoko emva kokuba ivuliwe.

IZILUMKISO NEZILUMKISO
.Ikhithi kufuneka ivalwe kwaye ikhuselwe kumswakama.
Zonke iisampulu ezilungileyo maziqinisekiswe ngezinye iindlela.
Zonke iisampulu maziphathwe njengezingcolisi ezinokubakho.
.MUSA ukusebenzisa i-reagent ephelelwe lixesha.
.MUSA ukutshintshanisa ii-reagents phakathi kweekiti ezinenani elahlukileyo.
.MUSA ukusebenzisa kwakhona amakhadi ovavanyo kunye nazo naziphi na izixhobo ezilahlwayo.
.Ukusetyenziswa gwenxa, isampuli egqithisileyo okanye encinci kunokubangela ukuphambuka kweziphumo.

LUKULINGANISA
.Njengakweyiphi na i-assay esebenzisa ii-antibodies zegundane, kunokwenzeka ukuba kubekho ukuphazamiseka kwee-antibodies ze-anti-mouse (HAMA) kwi-sampuli. Iisampuli ezivela kwizigulana ezifumene amalungiselelo e-monoclonal antibodies ukuze zixilongwe okanye zonyango zinokuba ne-HAMA. Ezi sampuli zinokubangela iziphumo ezilungileyo okanye ezimbi.
.Esi siphumo sovavanyo sesokubhekisela kwiklinikhi kuphela, akufuneki sisebenze njengesiseko sodwa sokuxilongwa kweklinikhi kunye nonyango, ulawulo lweklinikhi lwezigulane kufuneka luqwalaselwe ngokupheleleyo kunye neempawu zalo, imbali yezonyango, olunye uvavanyo lwelabhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi.
.Le reagent isetyenziswa kuphela kuvavanyo lwelindle. Isenokungafumani ziphumo zichanekileyo xa isetyenziswa kwezinye iisampuli ezifana namathe nomchamo njl.

IIMPAWU ZOKUSEBENZA

RIINGXELO

1.Shao, JL&F.Wu. Inkqubela phambili yakutshanje kwiindlela zokufumanisa iHelicobacter pylori[J]. Ijenali yeGastroenterology kunye neHepatology, 2012,21(8):691-694
2.Hansen JH, et al. Ukuphazamiseka kwe-HAMA kwi-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
3.Levinson SS. Uhlobo lwee-Antibodies ze-Heterophilic kunye nendima ekuphazamisekeni kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Isitshixo seempawu ezisetyenzisiweyo:

	Isixhobo Sonyango Sokuxilonga se-In Vitro
	Umenzi
	Gcina kwi-2-30℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKISO
	Jonga Imiyalelo Yokusetyenziswa

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