Kete Tātaritanga mō te Antigen ki te Helicobacter Pylori(Whakamātautau Ārai-Kōromātohiko Hikoirangi)
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA
He pai te Kete Tātaritanga mō te Antigen ki te Helicobacter Pylori (Fluorescence Immunochromatographic Assay) mō te kimi i te nui o te tūtae tangata HP antigen mā te whakamātautau fluorescence immunochromatographic, he mea nui hei āwhina i te tātaritanga mō ngā mate puku. Me whakaū te tauira pai katoa mā ētahi atu tikanga. Ko tēnei whakamātautau he mea hanga mō te whakamahinga hauora anake.

WHAKARĀPOPOTONGA
He hononga tata te mate pukupuku Helicobacter pylori ki te gastritis mau tonu, te mate puku, te adenocarcinoma puku, te lymphoma e pā ana ki te mucosa puku, te tere o te mate Hp ylori tata ki te 90% i roto i te gastritis, te mate puku, te mate duodenal me te mate pukupuku puku. Kua tautuhia e te whakahaere hauora o te ao te h. pylori hei momo take tuatahi o te mate pukupuku, ā, he tino take mōrearea mō te mate pukupuku puku. He mea nui te kitenga i te H.pylori i roto i te tautuhi i te mate h. pylori. Ko te whakamātautau e hangai ana ki ngā tikanga tātari whakamātautau fluorescence immunochromatographic, ka taea te whakaputa hua i roto i te 15 meneti.

TE MĀTĀPONO O TE TIKANGA

Kei te rīpene he paturopi paninga anti-HP kei te rohe whakamātautau, e herea ana ki te chromatography membrane i mua. Ka pania te papa tapanga ki te paturopi anti-HP kua tohua ki te fluorescence i mua. I te whakamātautau i te tauira pai, ka taea te whakaranu i te HP i roto i te tauira me te paturopi anti-HP kua tohua ki te fluorescence, ka hanga he ranunga ārai mate. I te mea ka tukuna te ranunga kia heke i te rīpene whakamātautau, ka hopukina te matatini honohono HP e te paturopi paninga anti-HP i runga i te membrane ka hanga he matatini. He hononga pai te kaha o te fluorescence ki te ihirangi HP. Ka taea te kite i te HP i roto i te tauira mā te tātari immunoassay fluorescence.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA

Ngā wāhanga mōkihi 25T：
Kāri whakamātautau takitahi, he mea takai ki te pepa konutea me te whakamaroke 25T
Ngā wairewa tauira 25T
Tāpiringa mōkihi 1

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU
Ipu kohinga tauira, taima

TE KOHINGA TAUIRA ME TE ROKIROKI
1. Whakamahia he ipu ma, ka taea te whakamahi hei kohi i ngā tauira paru hou, ka whakamātauria tonutia. Ki te kore e taea te whakamātau tonutia, rongoatia ki te 2-8°C mō ngā rā e 3, ki raro iho rānei i te -15°C mō ngā marama e 6.
2. Tangohia te rākau tauira kua whakaurua ki roto i te tauira paru, ka mahi anō i te mahi kia toru ngā wā, ka tangohia ngā wāhanga rerekē o te tauira paru i ia wā, kātahi ka whakahokia te rākau tauira, ka whakapirihia kia mau, ka rūrū pai. Mā te whakamahi rānei i te rākau tauira, ka kōwhiria te tauira paru kia 50mg pea, ka whakauru ki roto i te ngongo tauira paru kei roto te waimeha tauira, ka whakapirihia kia mau.
3. Whakamahia he pipette hei tango tauira paru mai i te tūroro mate korere, kātahi ka tāpirihia kia 3 ngā pata (tata ki te 100µL) ki te ngongo tauira paru ka rūrū kia pai.

Ngā Tuhipoka:
1. Āta karohia ngā huringa tio-rewa.
2. Whakarewahia ngā tauira ki te pāmahana rūma i mua i te whakamahinga.

TE TIKANGA WHAKAMĀTAUTAU
Pānuihia te pukapuka whakahaere taputapu me te tāpiringa ki te kete i mua i te whakamātautau.
1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2. Whakatuwheratia te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101), tāuruhia te kupuhipa takiuru pūkete e ai ki te tikanga whakahaere o te taputapu, ka tomo ki te atanga kimi.
3. Matawaihia te waehere whakaū niho hei whakaū i te tūemi whakamātautau.
4. Tangohia te kāri whakamātautau i te putea pepa konutea.
5. Whakauruhia te kāri whakamātautau ki roto i te mokamoka kāri, matawaihia te waehere QR, ka kimihia te tūemi whakamātautau.
6. Tangohia te taupoki i te ngongo tauira ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 3 ngā pata (tata ki te 100µL) o te tauira kua whakaranua kia poutū, ka ringihia mārire ki roto i te poka tauira o te kāri me te ipu kua whakaratohia.
7. Pāwhiritia te pātene "whakamātautau paerewa", i muri i te 15 meneti, ka kitea aunoa e te taputapu te kāri whakamātautau, ka taea te pānui i ngā hua mai i te mata whakaatu o te taputapu, ā, ka tuhia/tāia ngā hua whakamātautau.
8. Tirohia ngā tohutohu mō te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101).

NGĀ UARA E TŪMANAKOHIA ANA
HP-Ag<10
E taunakihia ana kia whakatūhia e ia taiwhanga tōna ake awhe noa e tohu ana i te taupori tūroro.

NGĀ HUA WHAKAMĀTAUTAU ME TE WHAKAMĀRAMATANGA
1. Ko te HP-Ag i roto i te tauira he nui ake i te 10, ā, me whakakore te rerekētanga o te āhua ā-tinana. He tino rerekē ngā hua, ā, me tātaritanga me ngā tohu haumanu.
2. Ka pā noa ngā hua o tēnei tikanga ki ngā awhe tohutoro kua whakatūria i roto i tēnei tikanga, ā, kāore he whakataurite tika ki ētahi atu tikanga.
3. Ka taea hoki e ētahi atu āhuatanga te whakaputa hapa i ngā hua kimi, tae atu ki ngā take hangarau, ngā hapa whakahaere me ētahi atu āhuatanga tauira.

TE ROKIROKI ME TE PŪMAU
1. E 18 marama te roa o te oranga o te kete mai i te rā i hangaia ai. Penapenahia ngā kete kāore anō kia whakamahia ki te 2-30°C. KAUA E WHAKATIO. Kaua e whakamahia i tua atu i te rā paunga.
2. Kaua e huakina te putea hiri kia rite rā anō koe ki te mahi i tētahi whakamātautau, ā, e taunakihia ana kia whakamahia te whakamātautau kotahi-whakamahinga i raro i te taiao e hiahiatia ana (pāmahana 2-35℃, haumākū 40-90%) i roto i te 60 meneti i te wā tere rawa atu.
3. Ka whakamahia te wai whakamātao tauira i muri tonu i te whakatuwheratanga.

NGĀ WHAKATŪPATO ME NGĀ WHAKATŪPATO
Me hiri te kete, ā, me tiaki hoki i te makuku.
Me whakamana ngā tauira pai katoa mā ētahi atu tikanga.
Me whakaaro ngā tauira katoa he poke pea.
KAUA e whakamahia te matū kua pau te wā.
.KAUA e whakawhiti i ngā matū matū i waenga i ngā kete he rerekē te nama puranga.
KAUA e whakamahi anō i ngā kāri whakamātautau me ngā taputapu whakakorekore.
.Mēnā he te whakamahinga, he nui rawa, he iti rānei te tauira, ka rerekē pea ngā hua.

LWHAKATAURANGA
Pērā i ngā whakamātautau katoa e whakamahi ana i ngā paturopi kiore, tera pea ka pāngia te tauira e ngā paturopi ārai-kiore tangata (HAMA). Tērā pea kei roto i ngā tauira mai i ngā tūroro kua whiwhi i ngā whakaritenga o ngā paturopi monoclonal mō te tātaritanga, te rongoā rānei, te HAMA. Tērā pea ka puta he hua hē, he hua hē rānei.
Hei tohutoro haumanu anake tēnei hua whakamātautau, kaua e waiho hei pūtake anake mō te tātaritanga haumanu me te maimoatanga, me whai whakaaro whānui te whakahaere haumanu o ngā tūroro me ōna tohu, hītori hauora, ētahi atu whakamātautau taiwhanga, urupare maimoatanga, mate urutā me ētahi atu mōhiohio.
Ka whakamahia anake tēnei matū hei whakamātautau paru. Tērā pea kāore e tika te hua ina whakamahia mō ētahi atu tauira pēnei i te huware me te mimi me ētahi atu.

NGĀ ĀHUATANGA MAHI

RNGĀ WHAKAMĀRAMA

1.Shao,JL&F.Wu.Ngā whanaketanga hou o ngā tikanga kimi i te Helicobacter pylori[J].Journal of Gastroenterology and Hepatology,2012,21(8):691-694
2.Hansen JH, et al. Te Whakararuraru HAMA ki ngā Immunoassays Murine Monoclonal Antibody-Based Immunoassays[J].J o Clin Immunoassay,1993,16:294-299.
3.Levinson SS. Te Āhua o ngā Paturopi Heterophilic me te Tūranga i roto i te Whakararuraru Immunoassay[J].J o Clin Immunoassay,1992,15:108-114.

Ngā tohu matua i whakamahia:

	Pūrere Hauora Tātaritanga In Vitro
	Kaihanga
	Penapena ki te 2-30℃
	Rā Paunga
	Kaua e Whakamahi Anō
	WHAKATŪPATO
	Tirohia ngā Tohutohu Whakamahinga

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