Don amfani da ganewar asali kawai a cikin vitro

Da fatan za a karanta wannan fakitin da aka saka a hankali kafin amfani kuma a bi umarnin sosai. Ba za a iya tabbatar da ingancin sakamakon gwaji ba idan akwai wata matsala daga umarnin da ke cikin wannan fakitin.

AN YI NIYYA DA AMFANI

Kit ɗin Bincike don Maganin Hana Haihuwa ta Cutar Hepatitis C (Fluorescence Immunochromatographic Assay) gwajin fluorescence immunochromatographic ne don gano adadi na maganin rigakafi na HCV a cikin jini ko plasma na ɗan adam, wanda yake da mahimmanci don ganewar asali don kamuwa da cutar hepatitis C. Duk samfuran da aka tabbatar dole ne a tabbatar da su ta wasu hanyoyin. Wannan gwajin an yi shi ne don amfanin ƙwararrun ma'aikatan kiwon lafiya kawai.

1. A ajiye dukkan sinadaran da aka yi amfani da su da kuma samfuran da aka yi amfani da su a zafin ɗaki.
2. Buɗe Mai Nazarin Jiki Mai Ɗaukewa (WIZ-A101), shigar da kalmar sirri ta asusun bisa ga hanyar aiki ta kayan aikin, sannan ka shigar da hanyar ganowa.
3. Duba lambar haƙoran don tabbatar da abin gwajin.
4. Cire katin gwaji daga jakar foil.
5. Saka katin gwaji a cikin ramin katin, duba lambar QR, sannan ka tantance abin gwajin.
6. Ƙara samfurin serum ko plasma 20μL a cikin samfurin mai narkewa, sannan a gauraya sosai.
7. Ƙara maganin samfurin 80μL don yin samfurin rijiyar katin.
8. Danna maɓallin "gwaji na yau da kullun", bayan mintuna 15, kayan aikin zai gano katin gwaji ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aikin, kuma ya yi rikodin/buga sakamakon gwajin.
9. Duba umarnin Portable Immune Analyzer (WIZ-A101).

TAƘAITACCE

Kwayar cutar Hepatitis C (HCV) wata cuta ce mai suna envelope, wacce ke ɗauke da sigar RNA mai kyau (9.5 kb) wacce take cikin dangin Flaviviridae. An gano manyan nau'ikan halittu guda shida da jerin nau'ikan HCV. An ware HCV a shekarar 1989, kuma yanzu an gane ta a matsayin babban dalilin da ke haifar da cutar hepatitis wadda ba ta da A, wadda ba ta da B. Cutar tana da siffa mai tsanani da ta yau da kullun. Fiye da kashi 50% na mutanen da suka kamu da cutar suna kamuwa da cutar hepatitis mai tsanani mai barazana ga rayuwa tare da cirrhosis na hanta da kuma cutar kansar hanta. Tun lokacin da aka fara gwajin jini na anti-HCV a shekarar 1990, an rage yawan kamuwa da wannan cuta a cikin waɗanda suka karɓi wannan magani. Nazarin asibiti ya nuna cewa adadin mutanen da suka kamu da cutar HCV masu yawa suna haɓaka ƙwayoyin rigakafi ga furotin mara tsari na NS5 na ƙwayar cuta. Don wannan, gwaje-gwajen sun haɗa da antigens daga yankin NS5 na kwayar halittar kwayar cuta ban da NS3 (c200), NS4 (c200) da Core (c22).

KA'IDUN TSARIN

An shafa membrane na na'urar gwajin da antigen HCV a yankin gwaji da kuma antibody IgG antibody na akuya a yankin sarrafawa. Ana shafa faifan label da fluorescence mai lakabin HCV antigen da zomo IgG a gaba. Lokacin gwada samfurin tabbatacce, antibody na HCV a cikin samfurin yana haɗuwa da fluorescence mai lakabin HCV antigen, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun a cikin jagorancin takardar sha, lokacin da hadaddun ya wuce yankin gwaji, yana haɗuwa da antigen mai rufi na HCV, yana samar da sabon hadaddun. Matsayin antibody na HCV yana da alaƙa mai kyau da siginar fluorescence, kuma ana iya gano yawan antibody na HCV a cikin samfurin ta hanyar gwajin immunoassay na fluorescence.

MASU YIN REAGENTS DA KAYAN DA AKA KAWO

Kayan aikin fakitin 25T:
.Katin gwaji daban-daban an saka shi a cikin jaka da abin cirewa
.Samfurin masu narkewa
.Saka fakitin

ANA BUKATAR KAYAN AIKI AMMA BA A BA DA SU BA
Samfurin tarin akwati, mai ƙidayar lokaci
TARIN SAMFURI DA AJIYEWA
1. Samfuran da aka gwada na iya zama na jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2. Dangane da dabarun da aka saba amfani da su, ana iya ajiye samfurin jini ko na plasma a cikin firiji a zafin 2-8℃ na tsawon kwanaki 7, kuma ana iya ajiye shi a ƙasa da -15°C na tsawon watanni 6.
3. Duk samfurin da aka yi amfani da shi don guje wa daskarewa da narkewar zagayowar.

Tsarin Gwaji
Da fatan za a karanta littafin aikin kayan aiki da kuma abin da aka saka a cikin fakitin kafin a gwada.
.Wannan sakamakon gwajin an yi shi ne kawai don bayanin asibiti, bai kamata ya zama tushen ganewar asali da magani na asibiti ba, ya kamata a yi la'akari da kulawar marasa lafiya sosai tare da alamominsa, tarihin likita, sauran gwajin dakin gwaje-gwaje, amsawar magani, ilimin cututtuka da sauran bayanai.
.Wannan sinadarin ana amfani da shi ne kawai don gwaje-gwajen jini da na jini. Yana iya ba zai sami sakamako mai kyau ba idan aka yi amfani da shi ga wasu samfura kamar su yau da fitsari da sauransu.

HALAYEN AYYUKA

NAZARI
1. Ciwon hanta bayan an yi masa tiyata. A cikin: Moore SB, edita. Cututtukan Kwayar cuta da aka Yi wa Zubar da Ciki. Alington, VA. Am. Assoc. Blood Banks, shafi na 53-38.
2. Hansen JH, da sauransu. Tsoma bakin HAMA ga gwajin rigakafi na Murine Monoclonal Antibody-based [J].J na Clin Immunoassay, 1993,16:294-299.
3. Levinson SS. Yanayin Kwayoyin Haɗaka Masu Yawa da Matsayinsu a Cikin Tsangwama Kan Tsarin Immunoassay[J].J na Clin Immunoassay, 1992,15:108-114.
4.Alter HJ., Purcell RH, Holland PV, da sauransu (1978) Maganin da ake iya yadawa a cikin cutar hepatitis mara A, wadda ba ta B ba. Lancet I: 459-463.
5.Choo QL,Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Cutar Hepatitis C: babban abin da ke haifar da cutar hepatitis ba A ba, ba B ba. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Gwajin enzyme da aka haɗa da immunosorbent (ELISA): gwajin inganci na IgG. Immunochemistry 8:871-874.

DARAJOJI DA AKA YI TSAMMANI

HCV-Ab <0.02

Ana ba da shawarar kowane dakin gwaje-gwaje ya kafa nasa kewayon da ya dace wanda ke wakiltar yawan marasa lafiya.

SAKAMAKON GWAJI DA FASSARAR

• Bayanan da ke sama sakamakon gwajin reagent na HCV-Ab ne, kuma ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kamata ya kafa kewayon ƙimar gano HCV-Ab da ta dace da yawan jama'a a wannan yanki. Sakamakon da ke sama don tunani ne kawai.
• Sakamakon wannan hanyar ya shafi kawai ga jeri na tunani da aka kafa a cikin wannan hanyar, kuma babu wani kwatancen kai tsaye da wasu hanyoyi.
• Wasu dalilai kuma na iya haifar da kurakurai a sakamakon gano cutar, gami da dalilan fasaha, kurakuran aiki da sauran abubuwan samfurin.

Ajiya da kwanciyar hankali

1. Kayan aikin yana da tsawon rai na watanni 18 daga ranar da aka ƙera shi. Ajiye kayan da ba a yi amfani da su ba a zafin 2-30°C. KAR A DASKARE. Kada a yi amfani da su fiye da ranar karewa.
2. Kada a buɗe jakar da aka rufe har sai kun shirya yin gwaji, kuma ana ba da shawarar a yi amfani da gwajin amfani da shi sau ɗaya a ƙarƙashin yanayin da ake buƙata (zafin jiki 2-35℃, danshi 40-90%) cikin mintuna 60 da sauri.
3. Ana amfani da samfurin maganin shafawa nan da nan bayan an buɗe shi.

GARGAƊI DA HANKALI
.Ya kamata a rufe kayan aikin kuma a kare shi daga danshi.
Duk samfuran da aka tabbatar za a tabbatar da su ta wasu hanyoyi.
.Za a yi wa dukkan samfuran magani a matsayin masu iya gurɓata muhalli.
.KADA a yi amfani da maganin da ya ƙare.
.KADA a musanya sinadaran da ke cikin kayan aiki da kuma sauran kayan aiki.
.KADA a sake amfani da katunan gwaji da duk wani kayan haɗi da za a iya yarwa.
.Rashin aiki yadda ya kamata, ko kuma yawan samfur ko ƙarancin sample na iya haifar da karkacewar sakamako.

LKWAIKWAYO
.Kamar yadda yake a kowace gwaji da ake amfani da ƙwayoyin rigakafi na linzamin kwamfuta, akwai yiwuwar tsangwama daga ƙwayoyin rigakafi na ɗan adam (HAMA) a cikin samfurin. Samfuran marasa lafiya waɗanda aka yi musu shirye-shiryen ƙwayoyin rigakafi na monoclonal don ganewar asali ko magani na iya ƙunsar HAMA. Irin waɗannan samfuran na iya haifar da sakamako mara kyau ko mara kyau na ƙarya.
Maɓallin alamomin da aka yi amfani da su:

	Na'urar Lafiya ta In Vitro Diagnostic
	Mai ƙera
	Ajiye a 2-30℃
	Ranar Karewa
	Kada a sake amfani da shi
	HANKALI
	Duba Umarnin Amfani