No ka hoʻohana diagnostic in vitro wale nō

E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi.ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

KA MANAO ANA

ʻO ka Diagnostic Kit no ka Hepatitis C Virus Antibody (Fluorescence Immunochromatographic Assay) he hōʻike immunochromatographic fluorescence no ka ʻike nui ʻana o ka HCV antibody i loko o ka serum kanaka a i ʻole ka plasma, he mea koʻikoʻi ka waiwai diagnostic kōkua no ka maʻi me ka hepatitis C. nā ʻano hana.Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō

1. E waiho i nā reagents a me nā laʻana i ka lumi wela.
2. E wehe i ka Portable Immune Analyzer (WIZ-A101), e hoʻokomo i ka ʻōlelo huna huna e like me ke ʻano hana o ka mea kani, a komo i ka ʻike ʻike.
3. E nānā i ke code dentification e hōʻoia i ka mea hoʻāʻo.
4. E lawe i ke kāleka hōʻoia mai ka ʻeke pepa.
5. E hoʻokomo i ke kāleka hōʻike i loko o ka pahu kāleka, e nānā i ka QR code, a hoʻoholo i ka mea hōʻike.
6.Add 20μL serum a plasma hāpana e laʻana diluent, a hui pono..
7.Add 80μL hāpana hāpana no ka laʻana maikaʻi o ke kāleka.
8. E kaomi i ke pihi "ho'āʻo maʻamau", ma hope o 15 mau minuke, e ʻike koke ka mea hana i ka kāleka hōʻike, hiki iā ia ke heluhelu i nā hopena mai ka pale hōʻike o ka mea kani, a hoʻopaʻa / paʻi i nā hopena hōʻike.
9. E nānā i ke aʻo ʻana o Portable Immune Analyzer (WIZ-A101).

HOOLAHA

ʻO ka maʻi maʻi Hepatitis C (HCV) he envelope, hoʻokahi stranded positive sense RNA (9.5 kb) virus no ka ʻohana Flaviviridae.Ua ʻike ʻia nā ʻano genotype ʻeono a me nā ʻano subtypes o HCV.Hoʻokaʻawale ʻia i ka makahiki 1989, ʻike ʻia ʻo HCV ʻo ia ke kumu nui o ka maʻi hepatitis non-A, non-B.Hōʻike ʻia ka maʻi me ke ʻano acute a me ka maʻi.ʻOi aku ma mua o 50% o ka poʻe i loaʻa i ka maʻi i hoʻomohala ʻia i ka maʻi hepatitis maʻi hoʻoweliweli me ka cirrhosis ate a me nā maʻi hepatocellular carcinomas.Mai ka hoʻomaka ʻana i ka makahiki 1990 o ka hōʻike ʻana i ka anti-HCV i nā hāʻawi koko, ua hoʻemi nui ʻia ka nui o kēia maʻi i ka poʻe lawe i ka transfusion.Hōʻike nā haʻawina lāʻau lapaʻau i ka nui o ka poʻe i loaʻa i ka maʻi HCV e hoʻomohala i nā antibodies i ka NS5 non-structural protein o ka maʻi.No kēia, ua komo nā hoʻāʻo i nā antigens mai ka ʻāina NS5 o ka genome viral me ka NS3 (c200), NS4 (c200) a me ka Core (c22).

KUMU O KE HANA

Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka antigen HCV ma ka ʻāina hoʻāʻo a me ka anti rabbit IgG antibody ma ka ʻāina hoʻomalu.Hoʻopili ʻia ka papa inoa e ka fluorescence i kapa ʻia ʻo HCV antigen a me ka rabbit IgG ma mua.I ka ho'āʻo ʻana i ka laʻana maikaʻi, hui pū ka antibody HCV i ka laʻana me ka fluorescence i kapa ʻia ʻo HCV antigen, a hana ʻia ka hui ʻole.Ma lalo o ka hana o ka immunochromatography, ka paʻakikī kahe i ke kuhikuhi o absorbent pepa, i ka wā paʻakikī i hala i ka ho'āʻo māhele 'āina, ua hui me ka HCV antigen uhi antigen, hana hou complex.HCV antibody pae ua positively correlated me ka fluorescence hoailona, ​​a me ka noonoo ana o Hiki ke ʻike ʻia ka antibody HCV i ka laʻana e ka fluorescence immunoassay assay

NA REAGENA a me na lako i hoolakoia

25T pūʻolo mea：
. Kāleka hoʻāʻo pakahi i hoʻopaʻa ʻia me kahi desiccant
.Laʻana diluents
.Hoʻokomo pūʻolo

MAKEMAKE IA NA MEA AKA AOLE I HAAWI MAI
Laʻana hōʻiliʻili ipu, manawa
KA OHI A ME KA WAIWAI
1. ʻO nā mea hoʻohālike i ho'āʻoʻia he serum, heparin anticoagulant plasma a iʻole EDTA anticoagulant plasma.
2.According i nā hana maʻamau e hōʻiliʻili i ka hāpana.Hiki ke mālama ʻia ka serum a i ʻole plasma sample i loko o ka friji ma 2-8 ℃ no 7 mau lā a me ka cryopreservation ma lalo o -15 ° C no 6 mau mahina.
3.All laʻana e pale i nā pōʻaiapuni freeze-thaw.

HANA HOIKE
E ʻoluʻolu e heluhelu i ka manual operation instrument a me ka hoʻokomo pūʻolo ma mua o ka hoʻāʻo ʻana.
ʻO kēia hopena hoʻokolohua wale nō no ka hōʻike lapaʻau, ʻaʻole pono e lilo i kumu hoʻokahi no ka maʻi maʻi a me ka mālama ʻana, pono e noʻonoʻo ʻia nā mea maʻi i ka mālama ʻana i ka maʻi me kāna mau hōʻailona, ​​ka mōʻaukala olakino, nā hoʻokolohua ʻē aʻe, ka pane lapaʻau, epidemiology a me nā ʻike ʻē aʻe. .
.Hoʻohana wale ʻia kēia reagent no nā hoʻokolohua serum a me ka plasma.ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.

NA HIO HANA

HOOLAHA
1. Ma hope o ka hoʻoili ʻia ʻana o ka maʻi hepatitis.In: Moore SB, ed.ʻO nā maʻi maʻi maʻi i hoʻoili ʻia.Alington, VA.ʻO Am.Ua haʻi aku ʻo Assoc.Pahu koko, pp. 53-38.
2.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
3.Levinson SS.Ke 'ano o Heterophilic Antibodies a me ke kuleana i ka Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.
4. Alter HJ., Purcell RH, Holland PV, a me al.(1978) He mea hiki ke hoʻololi i ka maʻi hepatitis non-A, non-B.Lancet I: 459-463.
5.Choo QL, Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Hepatitis C Virus: ka mea kumu kumu nui o ka ma'i hepatitis non-A, non-B.Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Enzyme linked immunosorbent assay (ELISA): ho'āʻo qualitative o IgG.Immunochemistry 8:871-874.

NA WAIWAI I MANAOIA

HCV-Ab<0.02

Manaʻo ʻia e hoʻokumu kēlā me kēia keʻena hana i kona ʻano maʻamau e hōʻike ana i kona heluna maʻi.

HUA HOʻOʻO A ME KA HOʻOKAʻANA

• ʻO ka ʻikepili ma luna ka hopena o ka hoʻāʻo HCV-Ab reagent, a ua manaʻo ʻia e hoʻokumu kēlā me kēia keʻena keʻena i kahi ʻano o nā waiwai ʻike HCV-Ab kūpono i ka heluna kanaka ma kēia māhele.ʻO nā hualoaʻa ma luna nei no ka kuhikuhi wale nō.
• Hoʻopili wale nā ​​hopena o kēia ʻano i nā pae kuhikuhi i hoʻokumu ʻia ma kēia ʻano, a ʻaʻohe hoʻohālikelike pololei me nā ʻano hana ʻē aʻe.
• Hiki i nā kumu ʻē aʻe ke hana i nā hewa i nā hopena ʻike, me nā kumu ʻenehana, nā hewa hana a me nā kumu hoʻohālike ʻē aʻe.

HOOLAHA A ME KA PALAPALA

1. ʻO ka pahu he 18 mahina mālama ola mai ka lā i hana ʻia ai.E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30 ° C.MAI HOOMAIKAI.Mai hoʻohana ma mua o ka lā pau.
2. Mai wehe i ka ʻeke i hoʻopaʻa ʻia a mākaukau ʻoe e hana i kahi hoʻāʻo, a manaʻo ʻia ka hoʻohana hoʻokahi hoʻohana e hoʻohana ʻia ma lalo o ke kaiapuni i koi ʻia (ka mahana 2-35 ℃, haʻahaʻa 40-90%) i loko o 60 mau minuke me ka wikiwiki. .
3. Hoʻohana ʻia ka laʻana diluent ma hope koke o ka wehe ʻana.

OLELO HOOLAHA A ME KA MALAMA
. Pono e hoʻopaʻa ʻia ka pahu a pale ʻia mai ka makū.
.E hōʻoia ʻia nā hōʻailona maikaʻi a pau e nā ʻano hana ʻē aʻe.
.E mālama ʻia nā mea hoʻohālike a pau ma ke ʻano he mea haumia.
.MAI hoʻohana i ka reagent pau.
.Mai hoʻololi i nā reagents ma waena o nā pahu me nā ʻāpana ʻokoʻa No..
.Mai hoʻohana hou i nā kāleka hoʻāʻo a me nā mea pono hoʻopau.
.ʻO ka hana hewa ʻole, ka nui a i ʻole ka liʻiliʻi o ka laʻana hiki ke alakaʻi i ka hopena.

LHOOLAHA
.E like me ka hoʻohana ʻana i nā antibodies ʻiole, hiki ke hoʻopilikia ʻia e nā anti-mouse antibodies (HAMA) i loko o ka specimen.Hiki ke loaʻa ka HAMA i nā hōʻailona mai nā maʻi i loaʻa ka hoʻomākaukau ʻana o nā antibodies monoclonal no ka maʻi a i ʻole ka lāʻau lapaʻau.Hiki i ia mau hiʻohiʻona ke hoʻopuka i nā hopena maikaʻi ʻole a i ʻole nā ​​hopena maikaʻi ʻole.
Ki i nā hōʻailona i hoʻohana ʻia:

	In Vitro Diagnostic Lapaʻau Lapaʻau
	Mea hana
	E mālama i ka 2-30 ℃
	Ka lā pau
	Mai hoohana hou
	ALOHA
	E kūkākūkā i nā kuhikuhi no ka hoʻohana ʻana