Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa

Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE

Agakoresho ko gusuzuma indwara ya Hepatite C Antibody (Fluorescence Immunochromatographic Assay) ni ikizamini cya fluorescence immunochromatographic assay cyo gupima ingano ya antibody ya HCV mu maraso y'umuntu cyangwa mu maraso, kikaba ari ingenzi mu gusuzuma indwara ya hepatite C. Ingero zose zagaragaye zigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
4. Kura ikarita yo gupima mu gafuka k'ifishi.
5. Shyiramo ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
6. Ongeramo 20μL ya serum cyangwa plasma mu gipimo cy'icyitegererezo, hanyuma uvange neza.
7. Ongeraho umuti w'icyitegererezo wa 80μL ku iriba ry'ikarita.
8. Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 15, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
9. Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

INSHAMAKE

Virusi ya Hepatite C (HCV) ni virusi ifite ishusho imwe ya RNA (9.5 kb) ikomoka mu muryango wa Flaviviridae. Hamaze kumenyekana ubwoko butandatu bw’uturemangingo tw’amaraso n’uruhererekane rw’uturemangingo twa HCV. HCV yakuwe mu 1989, ubu izwiho kuba impamvu nyamukuru itera indwara ya hepatite itari A, itari B iterwa amaraso. Iyi ndwara irangwa n’imiterere ikabije n’iy’igihe kirekire. Abantu barenga 50% banduye barwara hepatite idakira ikomeye kandi ishobora guhitana ubuzima, ikaba ifite cirrhose y’umwijima na kanseri y’umwijima. Kuva aho hatangiriye mu 1990, umubare w’abanduye iyi ndwara mu bahabwa amaraso waragabanutse cyane. Ubushakashatsi bw’ubuvuzi bwerekana ko umubare munini w’abantu banduye HCV batera ubudahangarwa kuri poroteyine idakora ya NS5. Kuri iyi mpamvu, ibizamini birimo antigens zo mu gace ka NS5 ka genome ya virusi hiyongereyeho NS3 (c200), NS4 (c200) na Core (c22).

IHAME RY'UBURYO BWAYO

Agace k'igikoresho cyo gupima gatwikiriwe na antigen ya HCV ku gace k'igeragezwa naho antibody ya IgG yo mu ihene irwanya inkwavu ku gace k'igenzura. Udupira two gutwikiriwe na antigen ya HCV iriho fluorescence na IgG y'urukwavu mbere y'igihe. Mu gusuzuma icyitegererezo cyagaragaye, antibody ya HCV iri mu cyitegererezo ihuzwa na antigen ya HCV iriho fluorescence, maze igakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'imikorere ya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo complex yanyuze mu gace k'igeragezwa, yahujwe na antigen ya HCV, ikora complex nshya. Urwego rw'antibody ya HCV rufitanye isano ryiza n'ikimenyetso cya fluorescence, kandi ingano ya antibody ya HCV mu cyitegererezo ishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw'umubiri rya fluorescence.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:
Ikarita yo gupima ku giti cyayo ipfunyitse mu gapfunyika gafite agakoresho ko gusukura
.Ingero z'ibinyabutabire
.Ipaki yo gushyiramo

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe
ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
1. Ingero zapimwe zishobora kuba serum, heparin anticoagulant plasma cyangwa EDTA anticoagulant plasma.
2. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6
3. Ingero zose zirinda gukonjesha no gushonga.

UBURYO BWO GUKORA IKIGANIRO
Mbere yo gupima, soma igitabo cy'amabwiriza agenga imikorere y'igikoresho n'agasanduku k'ibikoresho.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwaba buhagaze, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima amaraso mu maraso no mu maraso. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.

IBIRANGA IMIKORERE

INYANDIKO Z'IBIREGO
1. Hepatite nyuma yo guterwa amaraso. Muri: Moore SB, ed. Indwara za Virusi zandurira mu guterwa amaraso. Alington, VA. Am. Assoc. Amasasu, pp. 53-38.
2.Hansen JH, et al.HAMA Interference hamwe n'ibipimo by'imyuka bishingiye ku murine monoclonal antibody [J].J bya Clin Immunoassay, 1993,16:294-299.
3.Levinson SS. Imiterere y'uturemangingo tw'umubiri duto duto n'uruhare mu gutera ikibazo cy'ubudahangarwa bw'umubiri [J].J ya Clin Immunoassay, 1992,15:108-114.
4.Alter HJ., Purcell RH, Holland PV, n'abandi (1978) Ishobora kwanduzanya mu ndwara ya hepatite itari iya A, itari iya B. Lancet I: 459-463.
5.Choo QL,Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Virusi ya Hepatite C: itera indwara ya virusi itari iya A, itari iya B. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Isuzuma rya enzyme linked immunosorbent assay (ELISA): isuzuma ry’imiterere ya IgG. Immunochemistry 8:871-874.

INDANGAGACIRO ZITEGANYIJWE

HCV-Ab<0.02

Birasabwa ko buri laboratwari ishyiraho urwego rwayo rusanzwe rugaragaza umubare w’abarwayi bayo.

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO

• Amakuru yavuzwe haruguru ni ibyavuye mu isuzuma rya HCV-Ab, kandi birasabwa ko buri laboratwari igomba gushyiraho ubwoko butandukanye bw'ibipimo byo gupima HCV-Ab bikwiranye n'abaturage bo muri aka karere. Ibisubizo byavuzwe haruguru ni ibyo kwifashisha gusa.
• Ibisubizo by'ubu buryo bireba gusa ingero z'ingero zashyizweho muri ubu buryo, kandi nta kugereranya kutaziguye n'ubundi buryo.
• Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE

1. Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ikozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
2. Ntukingure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
3. Icyitegererezo cyo gushonga gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
.Isanduku igomba kuba ifunze kandi irinzwe ubushuhe.
Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
.Ingero zose z'ibipimo bigomba gufatwa nk'ibintu bishobora kwanduza.
.NTUKORESHE reagent yarengeje igihe.
.NTUGAHINDURE ibyuma bihinduranya ibikoresho hagati y'ibikoresho bifite ubwoko butandukanye No.
.NTUGAKORESHE gukoresha amakarita yo gupima n'ibindi bikoresho byo gukoresha mu gihe cyo kuyakoresha.
.Gukoresha nabi, urugero rurenze urugero cyangwa ruto bishobora gutuma ibisubizo bitagenda neza.

LKWIGANA
.Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha