Ku itariki ya 19 Gicurasi ni umunsi mpuzamahanga w’icyorezo cya SIDA, igamije gukangurira abaturage indwara y’ububyimbirwe bw’amara (IBD) no kwita ku barwayi ba IBD. IBD ikubiyemo cyane cyane indwara ya kanseri y’ibisebe (UC) na indwara ya Crohn (CD). Ni indwara idakira, isubira inyuma y’amara igabanya cyane ubuzima bw’abarwayi. Kuri iyi nshuro, twishimiye gusangiza abandi ubushakashatsi buheruka bwakozwe n’ibitaro bya Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, byakoreshejeIkizamini cya Calprotectin Assay Kit(Ikizamini cya Fluorescence Immunochromatography Assay, FICA)n'Isesengura ry'ubudahangarwa bw'umubiri rya WIZ-A202 rihorahotwakoze, dutanga amakuru y'ingenzi ku bijyanye no kuvura indwara z'abarwayi ba UC.
Incamake y'ubushakashatsi: Gushaka ibipimo byiza byo gukurikirana bidakoresha uburyo busanzwe kuri UC
Colonoscopy ni "igipimo ngenderwaho cy'agaciro" cyo gusuzuma imikorere y'indwara zo mu nda muri UC, ariko irabangamira abarwayi kandi ntibemererwa neza, bigatuma gukurikirana kenshi bigorana. Ibipimo by'amaraso nkaPoroteyine ikora C (CRP) kandi igipimo cy’amaraso ya erythrocyte sedimentation (ESR) nta buryo bwihariye bwo kubyimba mu mara kandi ntibifitanye isano n’ububyimba bw’amara.
Kalprotectin y'amase (FC), ikimenyetso cy’ububyimbirwe bw’amara gikomoka kuri neutrophils, cyabaye igikoresho cy’ingenzi kidatera indwara mu micungire ya IBD. Ariko, ibipimo bya FC biratandukanye cyane mu buryo butandukanye bwo kuvumbura bitewe n’uko nta gaciro gasanzwe gakoreshwa mu gusuzuma indwara. Isuzuma rya Fluorescence immunochromatography assay (FICA) ni uburyo bworoshye kandi bwihuse bwo kuvumbura indwara, ariko ibipimo byayo byiza byo kuvura indwara bisaba ko igenzurwa kurushaho.
Intego n'uburyo bw'ubushakashatsi
1) Intego z'ubushakashatsi: Ubu bushakashatsi bwari bugamije gusuzuma isano iri hagati ya FC yavumbuwe na FICA n'imikorere y'indwara zo mu nda/endoskopi muri UC, no kumenya agaciro k'ingenzi ko kumenya imikorere y'indwara.
2） Umubare w’abarwayi bakoreweho ubushakashatsi: abarwayi 110 basanzwemo indwara ya UC bari mu bitaro bya Union Hospital, Tongji Medical College, Huazhong University of Science and Technology hagati ya Mutarama 2021 na Kamena 2022.
3）Uburyo bwo Gupima: FC yapimwe hakoreshejweIsesengura ry'ubudahangarwa bw'umubiri rya WIZ-A202 rihoraho n'ibijyanyeKit ya Calprotectin FICAkuva kuri twe
4) Ibipimo by'isuzuma: Ibikorwa bya muganga byapimwe hakoreshejwe amanota ya Mayo (pMS); ibikorwa bya endoscope byapimwe hakoreshejwe Ulcerative Colitis Endoscopic Index of Severity (UCEIS) na Mayo Endoscopic Score (MES).
Ibisubizo by'ubushakashatsi bw'ibanze
1. Ingano ya FC ifitanye isano rikomeye n'imikorere y'indwara.
Urwego rwa FC rwagaragaje isano ikomeye na pMS (r=0.609), UCEIS (r=0.751), na MES (r=0.635) (byose P<0.001), hamwe n'isano iri imbere kurushaCRPna ESR. Isano iri hagati ya FC n'imikorere ya endoskopi yari ikomeye cyane.
2. Ingano nziza z'ifatizo zigenwa.
| Intego yo guhanura | Uburyo bwiza bwo kugabanya (μg/g) | Ubuhanga bwo kwiyumvisha ibintu | Ubwihariye |
|---|---|---|---|
| Ibikorwa byo kwa muganga (pMS ≥ 3) | ≥57.38 | 78.02% | 100% |
| Igikorwa cya endoskopi (UCEIS ≥ 1 / MES ≥ 1) | ≥53.30 | 71.57–73.74% | 100% |
3. FC yagaragaje imikorere myiza mu gutandukanya UC ikora.
Isesengura rya ROC ryagaragaje ko AUC ya FC mu guhanura ibikorwa by'ubuvuzi yari 0.9456, naho mu guhanura ibikorwa bya endoskopi yari 0.9075 (UCEIS) na 0.9118 (MES), byose biruta cyane CRP na ESR.
4. Gusubira mu buzima ntabwo bingana no gusubira mu buryo bwa endoscopic.
Mu barwayi 11 babashije kugabanya ububabare bw'umubiri (pMS < 3) ariko bagifite ibikorwa byo gupima, 10 (90.91%) bari bafite urugero rwa FC ruri munsi ya 53.30 μg/g. Ibi bigaragaza ko nubwo urugero rwa FC ari rusanzwe, bamwe mu barwayi bashobora kuba batarabashije gukira, kandi koroskopiya idashobora gusimburwa burundu.
Umwanzuro w'ubushakashatsi
- Gupima FC bishingiye kuri FICA ni ikimenyetso cyiza cy'imikorere y'ubuvuzi n'imikorere y'inyuma mu buvuzi bwa UC.
- Kugira ngo hamenyekane imikorere y'ubuvuzi, hagomba kugabanywa 57.38 μg/g.
- Kugira ngo hamenyekane imikorere ya endoscope, hagomba kugabanywa 53.30 μg/g (urugero ruri hasi rufasha kongera ubushobozi bwo kumva).
- FC ishobora kugabanya neza uburyo bwo gupima indwara za colonoscopy bitari ngombwa no kunoza ikoreshwa ry'ibikoresho by'ubuvuzi.
Guteza imbere uburyo bwo gusuzuma no kuvura indwara ya IBD-Baysen neza
Xiamen Baysen Medical yateye imbere ku giti cyayoIkizamini cya Calprotectin Assay Kit (Isuzuma rya Fluorescence Immunochromatography Assay) naIsesengura ry'ubudahangarwa bw'umubiri rya WIZ-A202 rihorahobitanga ibyiza bikurikira:
Ikizamini cya Calprotectin Assay Kit (Ikizamini cya Fluorescence Immunochromatography)
Aho uherereye: Gupima ingano y'ubuvuzi
FICA ikoresha uburyo bwa sandwich bugizwe na antibody ebyiri kugira ngo imenye FC, igaragaza isano iri hagati yayo na ELISA, kandi ishobora gusuzuma neza imikorere ya IBD.
Ibyiza by'ibicuruzwa:
- Byihuse kandi byoroshye:Ibisubizo biraboneka mu minota 15 gusa
- Uburyo bworoshye bwo gukoraNta bikoresho bigoye bikenewe; bikwiranye n'ibigo by'ubuvuzi ku nzego zose
- Ibisubizo byizewe:Isano nziza n'ibikorwa byo kwa muganga n'ibyo mu nda
- Ibisabwa by'icyitegererezo bisobanutse neza: Uburyo bwo gupima buri gihe butuma habaho ireme ry'ibizamini
Porogaramu:Gusuzuma abarwayi bo hanze, gukurikirana abarwayi bari mu bitaro, gukurikirana ubuvuzi, no gukorana mu bushakashatsi
Ibyiza by'ibicuruzwa:
- Uburyo bwuzuye bwo guhuza ubwoko bw'icyitegererezo: Ushoboye gupima amaraso, inkari, n'umwanda
- Guhuza neza: Ifasha mu gukora isuzuma rya "colloidal gold method", "latex method", no gukora isuzuma rya "fluorescence immunoassay"
- Umutekano mu by’ibinyabuzima:Gukusanya amakarita y'imyanda mu buryo bwikora kugira ngo yoroshye kandi yitezweho
- Gutanga ingero zihoraho:Ifite icyumba cyo gufatiramo ibikoresho gifite imyanya myinshi, gituma ingero zishyirwamo kandi zigapimwa nta nkomyi.
- Kumenya mu buryo bw'ubwenge: Igaragaza mu buryo bwikora ibintu by'ibizamini n'uburyo bwo kubikoresha
- Uburyo bwo gukorana n'amakuru:Ifasha guhuza na LIS y'ibitaro (Sisitemu y'amakuru ya Laboratwari)
Ubu bushakashatsi bwemeza akamaro k’ingenzi ko gupima FICA-based FC mu buvuzi bwa UC, butanga igikoresho gikomeye cyo gukurikirana abarwayi ba IBD mu buryo butabangamira ubwiyongere bw’indwara.
Ku isi yose, umubare w’abandura indwara ya IBD ukomeje kwiyongera. Gusuzuma indwara hakiri kare, kuvurwa mu buryo busanzwe, no gukurikirana mu buryo bwa siyansi ni ingenzi mu kunoza umusaruro w’abarwayi. Nk’ikimenyetso cy’indwara kidakunze kwibasira, cyoroshye kandi cyihariye, calprotectin y’umwanda irimo guhindura uburyo bwo gucunga indwara ya IBD.
Twebwe Baysen/Wizbiotech dukomeje gukora ubushakashatsi no kunoza imiti ikoreshwa mu gusuzuma indwara ya IBD, guha abaganga n'abarwayi ibisubizo byo gupima neza kandi byoroshye, no gukorera hamwe mu kurinda ubuzima bw'amara.
Igihe cyo kohereza: Gicurasi-19-2026