Kugwiritsa ntchito poyezetsa matenda m'thupi kokha

Chonde werengani bwino phukusili musanagwiritse ntchito ndipo tsatirani malangizo mosamala. Kudalirika kwa zotsatira za mayeso sikungatsimikizidwe ngati pali kusiyana kulikonse kuchokera ku malangizo omwe ali mu phukusili.

KUGWIRITSA NTCHITO KOFUNIKA

Zida Zodziwira Matenda a Hepatitis C Virus Antibody (Fluorescence Immunochromatographic Assay) ndi njira yodziwira kuchuluka kwa ma antibodies a HCV mu seramu ya anthu kapena plasma, yomwe ndi yofunika kwambiri pozindikira matenda a hepatitis C. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Mayesowa ndi ogwiritsidwa ntchito ndi akatswiri azaumoyo okha.

1. Ikani pambali zinthu zonse zoyeretsera ndi zitsanzo kutentha kwa chipinda.
2. Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi olowera mu akaunti malinga ndi njira yogwirira ntchito ya chidacho, ndikulowetsa mawonekedwe ozindikira.
3.Scan code yodziwitsa kuti mutsimikizire chinthu choyesera.
4. Tulutsani khadi loyesera m'thumba la foil.
5. Ikani khadi loyesera mu malo oikamo khadi, sikani QR code, ndikuzindikira chinthu choyesera.
6. Onjezani 20μL seramu kapena chitsanzo cha plasma ku chitsanzo cha diluent, ndipo sakanizani bwino.
7. Onjezani yankho la chitsanzo cha 80μL ku chitsime cha chitsanzo cha khadi.
8. Dinani batani la "standard test", patatha mphindi 15, chidacho chidzazindikira khadi loyesera lokha, chimatha kuwerenga zotsatira kuchokera pazenera lowonetsera chidacho, ndikulemba/kusindikiza zotsatira za mayeso.
9. Onani malangizo a Portable Immune Analyzer (WIZ-A101).

CHIDULE

Kachilombo ka Hepatitis C (HCV) ndi kachilombo kofanana ndi ka RNA (9.5 kb) kamene kali m'gulu la Flaviviridae. Mitundu isanu ndi umodzi yayikulu ya majini ndi mitundu yosiyanasiyana ya HCV yapezeka. HCV yomwe idapezeka mu 1989, tsopano yadziwika kuti ndi chifukwa chachikulu cha matenda a chiwindi omwe si a A, B. Matendawa amadziwika ndi mawonekedwe a pachimake komanso osatha. Anthu opitilira 50% omwe ali ndi kachilomboka amakhala ndi matenda a chiwindi oopsa komanso oopsa omwe amapha anthu omwe ali ndi matenda a chiwindi komanso khansa ya chiwindi. Kuyambira pomwe mayeso a anti-HCV adayambitsidwa mu 1990, kuchuluka kwa matendawa kwa anthu omwe amalandira magazi kwachepa kwambiri. Kafukufuku wazachipatala akuwonetsa kuti anthu ambiri omwe ali ndi kachilombo ka HCV amapanga ma antibodies ku NS5 non-structural protein ya kachilomboka. Pachifukwa ichi, mayesowa akuphatikizapo ma antigen ochokera ku NS5 region ya viral genome kuwonjezera pa NS3 (c200), NS4 (c200) ndi Core (c22).

MFUNDO YA NJIRAYI

Nembanemba ya chipangizo choyesera imakutidwa ndi antigen ya HCV pamalo oyesera ndipo antibody ya IgG ya mbuzi pamalo olamulira. Mapepala olembera amakutidwa ndi antigen ya HCV yolembedwa fluorescence ndi IgG ya kalulu pasadakhale. Poyesa chitsanzo chabwino, antibody ya HCV mu chitsanzo imaphatikizana ndi antigen ya HCV yolembedwa fluorescence, ndikupanga chisakanizo cha chitetezo chamthupi. Pogwiritsa ntchito immunochromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zidadutsa pamalo oyesera, zimaphatikizana ndi antigen ya HCV yophimba antigen, zimapanga zovuta zatsopano. Mlingo wa antibody ya HCV umagwirizanitsidwa bwino ndi chizindikiro cha fluorescence, ndipo kuchuluka kwa antibody ya HCV mu chitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.

ZOPANGIRA NDI ZIPANGIZO ZOPEREKEDWA

Zigawo za phukusi la 25T：
Khadi loyesera lokhala ndi foil payokha lokhala ndi desiccant
Zitsanzo za zosungunulira
.Choyika phukusi

Zipangizo zofunika koma siziperekedwa
Chidebe chosonkhanitsira zitsanzo, nthawi
Zitsanzo Zosonkhanitsira ndi Kusunga
1. Zitsanzo zomwe zayesedwa zitha kukhala seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2. Kutengera njira zodziwika bwino, sonkhanitsani chitsanzo. Seramu kapena plasma ikhoza kusungidwa mufiriji pa 2-8℃ kwa masiku 7 ndikusungidwa pansi pa -15°C kwa miyezi 6.
3. Zitsanzo zonse kupewa kuzizira ndi kusungunula madzi.

NJIRA YOYESERA
Chonde werengani buku la malangizo ogwiritsira ntchito chipangizocho ndi phukusi la phukusi musanayese.
Zotsatira za mayesowa ndi za chithandizo chamankhwala chokha, siziyenera kukhala maziko okhawo odziwira matenda ndi chithandizo chamankhwala, kuyang'anira odwala kuyenera kuganiziridwa mokwanira pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwina kwa labotale, yankho la chithandizo, matenda a epidemiology ndi zina zambiri.
Chogwiritsiridwa ntchitochi chimagwiritsidwa ntchito poyesa seramu ndi plasma yokha. Sichingapeze zotsatira zolondola chikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina zotero.

MAKHALIDWE A NTCHITO

MAWU OTHANDIZA
1. Matenda a chiwindi pambuyo pa kuikidwa magazi. Mu: Moore SB, ed. Matenda a kachilombo ofalitsidwa ndi kuikidwa magazi. Alington, VA. Am. Assoc. Mabanki a Magazi, tsamba 53-38.
2.Hansen JH, ndi ena. Kusokoneza kwa HAMA ndi Murine Monoclonal Antibody-Based Immunoassays [J].J wa Clin Immunoassay,1993,16:294-299.
3.Levinson SS. Mtundu wa Ma Antibodies Osatha ndi Udindo mu Kusokoneza kwa Immunoassay [J].J wa Clin Immunoassay,1992,15:108-114.
4.Alter HJ., Purcell RH, Holland PV, et al. (1978) Mankhwala opatsirana mu chiwindi chosakhala cha A, chosakhala cha B. Lancet I: 459-463.
5.Choo QL,Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Kachilombo ka Hepatitis C: chomwe chimayambitsa matenda a chiwindi omwe si a A, komanso osakhala a B. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Kuyesa kwa enzyme linked immunosorbent (ELISA):kuyesa kwa IgG. Immunochemistry 8:871-874.

MFUNDO ZOYEMBEKEZEDWA

HCV-Ab<0.02

Ndikofunikira kuti labotale iliyonse ikhazikitse kuchuluka kwake komwe kumayimira odwala ake.

Zotsatira za Mayeso ndi Kutanthauzira

• Deta yomwe ili pamwambapa ndi zotsatira za mayeso a reagent a HCV-Ab, ndipo akulangizidwa kuti labotale iliyonse ikhazikitse mitundu yosiyanasiyana ya HCV-Ab yoyenera anthu m'derali. Zotsatira zomwe zili pamwambapa ndi zongogwiritsidwa ntchito pongogwiritsa ntchito.
• Zotsatira za njira iyi zimagwira ntchito kokha pa ma reference ranges omwe akhazikitsidwa mu njira iyi, ndipo palibe kufananizidwa mwachindunji ndi njira zina.
• Zinthu zina zingayambitsenso zolakwika pakupeza zotsatira, kuphatikizapo zifukwa zaukadaulo, zolakwika pakugwira ntchito ndi zinthu zina zachitsanzo.

KUSUNGA NDI KUKHALA BWINO

1. Chidachi chimakhala ndi moyo wa miyezi 18 kuyambira tsiku lopangidwa. Sungani zida zomwe sizinagwiritsidwe ntchito pa kutentha kwa 2-30°C. MUSAZIYIKE MU MAFURI. Musagwiritse ntchito kupitirira tsiku lotha ntchito.
2. Musatsegule thumba lotsekedwa mpaka mutakonzeka kuchita mayeso, ndipo mayeso ogwiritsidwa ntchito kamodzi akulangizidwa kuti agwiritsidwe ntchito pamalo ofunikira (kutentha 2-35℃, chinyezi 40-90%) mkati mwa mphindi 60 mwachangu momwe mungathere.
3. Choyeretsera chachitsanzo chimagwiritsidwa ntchito nthawi yomweyo chikatsegulidwa.

MACHENJEZO NDI ZOCHENJEZERA
.Zida ziyenera kutsekedwa ndi kutetezedwa ku chinyezi.
Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga chilengedwe.
.MUSAGWIRITSE NTCHITO reagent yotha ntchito.
.MUSASINTHIRE ma reagent pakati pa zida zomwe zili ndi nambala yosiyana.
.MUSAGWIRITSE NTCHITO makhadi oyesera ndi zinthu zina zilizonse zogwiritsidwa ntchito ngati zinyalala.
.Kugwiritsa ntchito molakwika, kuchuluka kwa zitsanzo kapena zochepa kungayambitse kusintha kwa zotsatira.

LKUTSANZIRA
.Monga momwe zimakhalira ndi mayeso aliwonse omwe amagwiritsa ntchito ma antibodies a mbewa, pali kuthekera kwa kusokonezedwa ndi ma antibodies a anti-mbewa a anthu (HAMA) mu chitsanzocho. Zitsanzo kuchokera kwa odwala omwe adalandira ma antibodies a monoclonal kuti apeze matenda kapena chithandizo zitha kukhala ndi HAMA. Zitsanzo zotere zingayambitse zotsatira zabodza kapena zabodza.
Chinsinsi cha zizindikiro zomwe zagwiritsidwa ntchito:

	Chipangizo Chachipatala Chodziwitsira Matenda mu Vitro
	Wopanga
	Sungani pamalo otentha 2-30℃
	Tsiku lothera ntchito
	Musagwiritsenso ntchito
	CHENJEZO
	Onani Malangizo Ogwiritsira Ntchito