Mō te whakamahinga tātaritanga in vitro anake

Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA

Ko te Kete Tātaritanga mō te Ārai Mate Hepatitis C (Fluorescence Immunochromatographic Assay) he whakamātautau ā-ira fluorescence mō te kimi i te ārai mate HCV i roto i te toto tangata, i te toto toto rānei, he mea nui hei āwhina i te tātaritanga mō te mate hepatitis C. Me whakaū te tauira pai katoa mā ētahi atu tikanga. Ko tēnei whakamātautau he mea whakamahi noa mā ngā tohunga hauora.

1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2. Whakatuwheratia te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101), tāuruhia te kupuhipa takiuru pūkete e ai ki te tikanga whakahaere o te taputapu, ka tomo ki te atanga kimi.
3. Matawaihia te waehere whakaū niho hei whakaū i te tūemi whakamātautau.
4. Tangohia te kāri whakamātautau i te putea pepa konutea.
5. Whakauruhia te kāri whakamātautau ki roto i te mokamoka kāri, matawaihia te waehere QR, ka kimihia te tūemi whakamātautau.
6. Tāpirihia he 20μL o te tauira toto, te tauira plasma rānei ki te wai whakawera tauira, ā, whakaranua kia pai.
7. Tāpirihia he 80μL o te otinga tauira ki te puna tauira o te kāri.
8. Pāwhiritia te pātene "whakamātautau paerewa", i muri i te 15 meneti, ka kitea aunoa e te taputapu te kāri whakamātautau, ka taea te pānui i ngā hua mai i te mata whakaatu o te taputapu, ā, ka tuhia/tāia ngā hua whakamātautau.
9. Tirohia ngā tohutohu mō te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101).

WHAKARĀPOPOTONGA

Ko te huaketo Hepatitis C (HCV) he huaketo RNA kotahi-aho pai (9.5 kb) kei roto i te whānau Flaviviridae. E ono ngā momo ira matua me ngā momo momo HCV kua tautuhia. I wehea i te tau 1989, kua mōhiotia inaianei ko te HCV te tino take o te whakawhiti toto hepatitis kore-A, kore-B e pā ana ki te mate. He mate whakapeka me te mate mau tonu te āhua o tēnei mate. Neke atu i te 50% o ngā tāngata kua pangia ka pāngia e te mate hepatitis mau tonu kino, morearea ki te ora me te cirrhosis ate me ngā mate pukupuku hepatocellular. Mai i te whakaurunga i te tau 1990 o te tirotiro anti-HCV o ngā koha toto, kua tino heke te auau o tēnei mate i roto i ngā kaiwhiwhi toto. E whakaatu ana ngā rangahau haumanu he nui te hunga kua pangia e te HCV e whakawhanake ana i ngā paturopi ki te pūmua kore-hanganga NS5 o te huaketo. Mō tēnei, kei roto i ngā whakamātautau ngā antigens mai i te rohe NS5 o te ira huaketo me te NS3 (c200), NS4 (c200) me te Core (c22).

TE MĀTĀPONO O TE TIKANGA

Kua pania te kiriuhi o te taputapu whakamātautau ki te antigen HCV i te rohe whakamātautau, ā, kua pania te paturopi IgG rāpeti koati ki te rohe whakahaere. Kua pania te papa tapanga ki te antigen HCV me te IgG rāpeti kua tohua ki te fluorescence i mua i te whakamātautau. I te whakamātautau i te tauira pai, ka honoa te paturopi HCV i roto i te tauira ki te antigen HCV kua tohua ki te fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina whiti te matatini i te rohe whakamātautau, ka honoa ki te antigen paninga antigen HCV, ka hanga he matatini hou. He hononga pai te taumata paturopi HCV ki te tohu fluorescence, ā, ka taea te kite i te kukū o te paturopi HCV i roto i te tauira mā te whakamātautau immunoassay fluorescence.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA

Ngā wāhanga mōkihi 25T：
Kāri whakamātautau takitahi he pepa konutea kua kapi ki te whakamaroke
Ngā waiwhakarewa tauira
.Whakaurunga mōkihi

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU
Ipu kohinga tauira, taima
TE KOHINGA TAUIRA ME TE ROKIROKI
1. Ko ngā tauira i whakamatautauria he toto toto, he toto toto heparin anticoagulant, he toto toto EDTA anticoagulant rānei.
2. E ai ki ngā tikanga paerewa, kohia he tauira. Ka taea te pupuri i te tauira toto, i te tauira plasma rānei ki roto i te pouaka whakamātao i te 2-8℃ mō ngā rā e 7, ā, ka taea te pupuri i te tauira i raro i te -15°C mō ngā marama e 6.
3. Kaua ngā tauira katoa e huri i te huka me te rewa.

TE TIKANGA WHAKAMĀTAUTAU
Pānuihia te pukapuka whakahaere taputapu me te tāpiringa ki te kete i mua i te whakamātautau.
Hei tohutoro haumanu anake tēnei hua whakamātautau, kaua e waiho hei pūtake anake mō te tātaritanga haumanu me te maimoatanga, me whai whakaaro whānui te whakahaere haumanu o ngā tūroro me ōna tohu, hītori hauora, ētahi atu whakamātautau taiwhanga, urupare maimoatanga, mate urutā me ētahi atu mōhiohio.
Ka whakamahia anake tēnei matū hei whakamātautau i te toto me te toto toto. Tērā pea kāore e tika te hua ina whakamahia mō ētahi atu tauira pēnei i te huware me te mimi me ētahi atu.

NGĀ ĀHUATANGA MAHI

NGĀ TOHUTORO
1. He mate ate i muri i te whakawhiti toto. I roto i: Moore SB, ētita. Ngā Mate Huaketo i Tukua e te Whakawhiti Toto. Alington, VA. Am. Assoc. Blood Banks, pp. 53-38.
2.Hansen JH, et al. Te Whakararuraru HAMA ki ngā Immunoassays Murine Monoclonal Antibody-Based Immunoassays[J].J o Clin Immunoassay,1993,16:294-299.
3.Levinson SS. Te Āhua o ngā Paturopi Heterophilic me te Tūranga i roto i te Whakararuraru Immunoassay[J].J o Clin Immunoassay,1992,15:108-114.
4.Alter HJ., Purcell RH, Holland PV, et al. (1978) Kaihoko tuku i roto i te mate hepatitis kore-A, kore-B. Lancet I: 459-463.
5.Choo QL, Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Huaketo Hepatitis C: te pūtake matua o te mate hepatitis kore-A, kore-B. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Whakamātautau Ārai Mate e Hono Ana ki te Whākōkī (ELISA): whakamātautau kounga o te IgG. Immunochemistry 8:871-874.

NGĀ UARA E TŪMANAKOHIA ANA

HCV-Ab<0.02

E taunakihia ana kia whakatūhia e ia taiwhanga tōna ake awhe noa e tohu ana i te taupori tūroro.

NGĀ HUA WHAKAMĀTAUTAU ME TE WHAKAMĀRAMATANGA

• Ko ngā raraunga o runga ake nei he hua nō te whakamātautau matū HCV-Ab, ā, e kīia ana me whakatū e ia taiwhanga he whānuitanga o ngā uara kitenga HCV-Ab e tika ana mō te taupori o tēnei rohe. Hei tohutoro noa iho ēnei hua o runga ake nei.
• Ka pā noa ngā hua o tēnei tikanga ki ngā awhe tohutoro kua whakatūria i roto i tēnei tikanga, ā, kāore he whakataurite tika ki ētahi atu tikanga.
• Ka taea hoki e ētahi atu āhuatanga te whakararu i ngā hua kimi, tae atu ki ngā take hangarau, ngā hapa whakahaere me ētahi atu āhuatanga tauira.

TE ROKIROKI ME TE PŪMAU

1. E 18 marama te roa o te oranga o te kete mai i te rā i hangaia ai. Penapenahia ngā kete kāore anō kia whakamahia ki te 2-30°C. KAUA E WHAKATIO. Kaua e whakamahia i tua atu i te rā paunga.
2. Kaua e huakina te putea hiri kia rite rā anō koe ki te mahi i tētahi whakamātautau, ā, e taunakihia ana kia whakamahia te whakamātautau kotahi-whakamahinga i raro i te taiao e hiahiatia ana (pāmahana 2-35℃, haumākū 40-90%) i roto i te 60 meneti i te wā tere rawa atu.
3. Ka whakamahia te wai whakawera tauira i muri tonu i te whakatuwheratanga.

NGĀ WHAKATŪPATO ME NGĀ WHAKATŪPATO
Me hiri te kete, ā, me tiaki hoki i te makuku.
Me whakamana ngā tauira pai katoa mā ētahi atu tikanga.
.Me whakaaro ngā tauira katoa he poke pea.
.KAUA e whakamahia te matū kua pau te wā.
.KAUA e whakawhiti i ngā matū matū i waenga i ngā kete he rerekē te nama puranga.
.KAUA e whakamahia anō ngā kāri whakamātautau me ngā taputapu whakakorekore.
.Mā te whakamahinga hē, te nui rawa, te iti rānei o te tauira ka rerekē ngā hua.

LWHAKATAURANGA
.Pērā i ngā whakamātautau katoa e whakamahi ana i ngā paturopi kiore, tera pea ka pāngia te tauira e ngā paturopi ārai-kiore tangata (HAMA). Tērā pea kei roto i ngā tauira mai i ngā tūroro kua whiwhi i ngā whakaritenga o ngā paturopi monoclonal mō te tātaritanga, te rongoā rānei, he HAMA. Tērā pea ka puta he hua hē, he hua hē rānei.
Ngā tohu matua i whakamahia:

	Pūrere Hauora Tātaritanga In Vitro
	Kaihanga
	Penapena ki te 2-30℃
	Rā Paunga
	Kaua e Whakamahi Anō
	WHAKATŪPATO
	Tirohia ngā Tohutohu Whakamahinga