Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela

Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi Yokuxilonga ye-Hepatitis C Virus Antibody (I-Fluorescence Immunochromatographic Assay) iyi-fluorescence immunochromatographic assay yokuthola inani le-antibody ye-HCV ku-serum noma ku-plasma yomuntu, okuyinani elibalulekile lokuxilonga lokutheleleka nge-hepatitis C. Zonke isampula ezinhle kumele ziqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

1. Beka eceleni wonke ama-reagent namasampula ukuze izinga lokushisa legumbi lifinyelele ezingeni lokushisa legumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3.Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
6. Faka isampula ye-serum noma ye-plasma engu-20μL kusampula yokuxuba, bese uxuba kahle.
7. Engeza isisombululo sesampula esingu-80μL emthonjeni wesampula wekhadi.
8.Chofoza inkinobho ethi “ukuhlolwa okujwayelekile”, ngemva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
9.Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

ISIFINYEZO

Igciwane le-Hepatitis C (i-HCV) liyigciwane eliyi-RNA eyodwa (9.5 kb) elivela emndenini we-Flaviviridae. Kutholakale izinhlobo eziyisithupha ezinkulu ze-genotypes kanye nochungechunge lwezinhlobo ezincane ze-HCV. I-HCV, eyahlukaniswa ngo-1989, manje ibhekwa njengembangela enkulu ye-hepatitis engeyona i-A, engeyona i-B ehlobene nokumpontshelwa igazi. Lesi sifo sibonakala ngesimo esibi nesingapheli. Abantu abangaphezu kuka-50% abangenwe yileli gciwane bahlakulela i-hepatitis engapheli enzima, esongela ukuphila ene-cirrhosis yesibindi kanye ne-carcinoma ye-hepatocellular. Kusukela kwaqala ukuhlolwa kwegazi okulwa ne-HCV ngo-1990, ukwanda kwalokhu kutheleleka kwabathola igazi kuye kwehla kakhulu. Izifundo zemitholampilo zibonisa ukuthi inani elikhulu labantu abangenwe yi-HCV lakha ama-antibodies ku-NS5 non-structural protein yegciwane. Kulokhu, ukuhlolwa kufaka phakathi ama-antigen avela esifundeni se-NS5 se-genome yegciwane ngaphezu kwe-NS3 (c200), NS4 (c200) kanye ne-Core (c22).

ISIMISO SENQUBO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-antigen ye-HCV esifundeni sokuhlola kanye ne-antibody ye-IgG yembuzi elwa nonogwaja esifundeni sokulawula. Iphedi yelebula imbozwe nge-antigen ye-HCV ebhalwe nge-fluorescence kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-antibody ye-HCV esibonelweni ihlangana ne-antigen ye-HCV ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimunca, lapho i-complex idlula esifundeni sokuhlola, ihlanganiswe ne-antigen emboza i-antigen ye-HCV, yakha i-complex entsha. Izinga le-antibody ye-HCV lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukuhlushwa kwe-antibody ye-HCV esibonelweni kungatholakala nge-fluorescence immunoassay assay.

IZINTO EZINGENAYO NEZINTO EZINIKEZWE

Izingxenye zephakheji ezingama-25T：
Ikhadi lokuhlola ngalinye lifakwe ifoyili ngesikhwama se-desiccant
.Izibonelo zokunciphisa
.Iphakheji yokufaka

KUDINGEKA IZINTO EZISETSHENZISWAYO KODWA AZINIKEZWA
Isitsha sokuqoqa isampula, isibali sikhathi
IQOQO LEZIBONELO NOKUGCINA
1. Amasampula ahlolwe angaba yi-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2. Ngokwezindlela ezijwayelekile zokuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku eziyi-7 kanye nokugcinwa ngaphansi kuka-15°C izinyanga eziyi-6
3. Zonke izibonelo zigwema imijikelezo yokuqandisa nokuncibilikisa.

INQUBO YOKUHLOLA
Sicela ufunde incwadi yemiyalelo yokusebenza kwethuluzi kanye nephakheji ngaphambi kokuhlola.
.Lo mphumela wokuhlolwa ungowokubhekisela emtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kwezokwelapha kuphela, ukuphathwa kwezokwelapha kweziguli kufanele kucatshangelwe ngokuphelele kuhlanganiswe nezimpawu zaso, umlando wezokwelapha, okunye ukuhlolwa kwelabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi.
.Le reagent isetshenziswa kuphela ekuhlolweni kwe-serum kanye ne-plasma. Ingase ingatholi umphumela onembile uma isetshenziswa kwamanye amasampula afana namathe nomchamo njll.

IZIMALI ZOKUSEBENZA

IZINKOMBA
1. I-hepatitis yangemva kokumpontshelwa igazi. Ku: Moore SB, ed. Izifo Ezibangelwa Amagciwane Ezidluliselwa Ngegazi. Alington, VA. Am. Assoc. Amabhange Egazi, amakhasi 53-38.
2.Hansen JH, nabanye.Ukuphazamiseka kwe-HAMA nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
3.Levinson SS. Uhlobo Lwama-Antibodies Angama-Heterophilic kanye Nendima Ekuphazamisekeni Kwe-Immunoassay[J].J we-Clin Immunoassay,1992,15:108-114.
4.Alter HJ., Purcell RH, Holland PV, et al. (1978) I-ejenti ethelelekayo kwi-hepatitis engeyona i-A, engeyona i-B. I-Lancet I: 459-463.
5.Choo QL,Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Igciwane le-Hepatitis C: imbangela enkulu ye-hepatitis engeyona i-A, engeyona i-B ebangelwa yigciwane. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Ukuhlolwa kwe-immunosorbent okuxhunywe yi-enzyme (ELISA): ukuhlolwa kwe-qualitative kwe-IgG. I-Immunochemistry 8:871-874.

AMANANI ALINDELEKILE

I-HCV-Ab<0.02

Kunconywa ukuthi ilabhorethri ngayinye isungule ububanzi bayo obujwayelekile obumele iziguli zayo.

IMIPHUMELA YOKUHLOLA NOKUHLUKANISWA

• Idatha engenhla ingumphumela wokuhlolwa kwe-HCV-Ab reagent, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule uhla lwamanani okuthola i-HCV-Ab afanele abantu bakulesi sifunda. Imiphumela engenhla iwukubhekisela kuphela.
• Imiphumela yale ndlela isebenza kuphela kumabanga okubhekisela asungulwe kule ndlela, futhi akukho ukuqhathaniswa okuqondile nezinye izindlela.
• Ezinye izici zingadala amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza kanye nezinye izici zesampula.

ISITOKELO NOKUZINZIMA

1. Ikhithi ihlala izinyanga ezingu-18 kusukela ngosuku lokwenziwa kwayo. Gcina amakhithi angasetshenziswanga ku-2-30°C. UNGAWAQANDISI. Ungayisebenzisi ngemva kosuku lokuphelelwa yisikhathi.
2. Ungavuli isikhwama esivaliwe uze ulungele ukwenza isivivinyo, futhi isivivinyo sokusetshenziswa kanye sinconywa ukuthi sisetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa 2-35℃, umswakama 40-90%) zingakapheli imizuzu engama-60 ngokushesha okukhulu.
3. Isampula yokuxuba isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQAPHELO ZOKWENZA
.Ikhithi kufanele ivalwe futhi ivikelwe emswakama.
Zonke izibonelo ezinhle kufanele ziqinisekiswe ngezinye izindlela.
.Zonke izibonelo kufanele ziphathwe njengezingcolisi ezingaba khona.
.UNGASEBENZISI i-reagent ephelelwe yisikhathi.
.UNGASHINTSHI ama-reagent phakathi kwamakhithi anenombolo ehlukile.
.UNGASEBENZISI kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Ukusebenza kabi, isampula eningi kakhulu noma encane kungaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukuhlolwa okusebenzisa ama-antibodies egundane, kungenzeka ukuthi kukhona ukuphazamiseka kwama-antibodies e-anti-mouse (HAMA) esampula. Amasampula avela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal ukuze kutholakale isifo noma ukwelashwa angase abe ne-HAMA. Amasampula anjalo angabangela imiphumela emibi noma emibi engamanga.
Isihluthulelo sezimpawu ezisetshenzisiwe:

	Idivayisi Yezokwelapha Yokuxilonga E-In Vitro
	Umkhiqizi
	Gcina ku-2-30℃
	Usuku lokuphelelwa isikhathi
	Ungasebenzisi Kabusha
	ISEXWAYISO
	Bheka Imiyalelo Yokusebenzisa